Polymerase chain reaction (PCR) for the detection of Salmonella in artificially inoculated chicken meat

Santos, Luciana Ruschel dos; Nascimento, Vladimir Pinheiro do; Oliveira, Sı́lvia Dias de; Flores, Maria Luiza Rodrigues; Pontes, Alexandre Pontes; Ribeiro, A. R.; Salle, Carlos Tadeu Pippi; Lopes, Rita de Cássia Sobreira

doi:10.1590/s0036-46652001000500002

Cited by 24 publications

(22 citation statements)

References 6 publications

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“…Nowadays, PCR-based methods, in particular quantitative PCR, are used primarily to identify and quantify either pathogens or beneficial populations, including Bacillus sp., Campylobacter sp., Legionella sp., Pseudomonas sp., Salmonella sp., and Vibrio sp., etc., based on the 16S rRNA genes or their specific functional genes (Amri et al 2007;Anbazhagan et al 2010;Brolund et al 2010;dos Santos et al 2001;Fiume et al 2005;Goarant and Merien 2006;Le Dréan et al 2010;Maligoy et al 2008;Masco et al 2007;Mashsouf et al 2008;Nakano et al 2003;Postollec et al 2011;Sauer et al 2005;Smith and Osborn 2008;Sun et al 2009;Vanniasinkam et al 1999;Wehrle et al 2010). Development of multiple PCR to simultaneously detect common pathogens has also been recommended, such as the PCR methods developed to detect Enterobacteriaceae and clinically important bacteria (Cheng et al 2006;Lu et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Quick identification and quantification of Proteus mirabilis by polymerase chain reaction (PCR) assays

et al. 2012

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Proteus mirabilis is an opportunistic pathogen that can cause urinary tract infection in human beings. The accurate and rapid identification and quantification of P. mirabilis is necessary for early treatment. In this study, a pair of specific primers according to the conserved ureR sequence of P. mirabilis was designed and novel systems which consisted of a polymerase chain reaction (PCR) and a real-time PCR to identify and quantify P. mirabilis were developed. For the qualitative identification by ordinary PCR, a 225-bp DNA product was amplified from P. mirabilis and separated on an agarose gel. The corresponding DNA product is present in three P. mirabilis strains isolated from different geographical locations, but is absent in 20 strains representing 18 different species, including the ureR homolog contained Providencia stuartii and Escherichia coli strains, the other common pathogens Klebsiella sp., Edwarsiella sp., Vibrio sp., Enterobacter sp., and Escherichia sp., and other environmental bacteria Pseudomonas sp. and Acinetobacter sp. Proteus mirabilis at concentrations higher than 1.0× 10 3 CFU ml −1 was detectable by ordinary PCR; P. mirabilis at concentrations higher than 10 CFU ml −1 was quantified by real-time PCR. The specific, sensitive and time-efficient PCR methods were demonstrated to be applicable to rapid identification and quantification of P. mirabilis.

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Section: Introductionmentioning

confidence: 99%

Quick identification and quantification of Proteus mirabilis by polymerase chain reaction (PCR) assays

et al. 2012

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“…As amostras foram submetidas à extração de DNA no Laboratório de Biologia Molecular da UPIS por tratamento térmico, conforme metodologia adaptada de Santos et al (2001b). Para isso, amostras de 1 mL de CRV foram centrifugadas a 3200 g por 4 min e o sobrenadante foi descartado.…”

Section: Methodsunclassified

Prevalência de Salmonella spp. em suínos abatidos em um frigorífico do Distrito Federal determinada pela técnica de PCR

Silva

Gonçalves

Lazzari

et al. 2018

Braz. J. Food Technol.

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Este é um artigo publicado em acesso aberto (Open Access) sob a licença Creative Commons Attribution, que permite uso, distribuição e reprodução em qualquer meio, sem restrições desde que o trabalho original seja corretamente citado. Prevalência de Salmonella spp. em suínos abatidos em um frigorífico do Distrito Federal determinada pela técnica de PCR Prevalence of Salmonella spp. in slaughtered swine in the Federal District of Brazil, as determined by the PCR technique ResumoA cadeia de produção suína vem crescendo assim como a exigência do fornecimento de produtos de qualidade, isentos de qualquer risco para os consumidores. A Salmonella spp. representa um risco biológico e é apontada como o agente responsável por diversos casos de doenças transmitidas por alimentos. O suíno é considerado um importante reservatório de Salmonella spp., o que torna imprescindível o conhecimento da dinâmica dessa bactéria em toda a cadeia de produção suína. Este trabalho foi realizado com o objetivo de determinar a prevalência de Salmonella spp. em suínos abatidos no Distrito Federal. Para isso, foram coletadas 240 amostras de linfonodos mesentéricos (LM) e 240 swabs de carcaça (SC) em um abatedouro com inspeção distrital. As amostras foram submetidas à PCR (Reação em Cadeia de Polimerase) para detecção da bactéria, revelando uma prevalência de 68,75% em LM de suínos abatidos e de 29,17% em SC suína. A análise dos dados obtidos demonstrou que a distância entre granja de origem e abatedouro apresentou correlação positiva moderada com a prevalência de Salmonella spp. em LM de suínos abatidos. Foi constatada, ainda, uma correlação positiva fraca entre a prevalência de Salmonella spp. em LM de suínos abatidos e a prevalência de Salmonella spp. em SC. Esses resultados apontam a necessidade da implantação de programas de controle para diminuir a prevalência nos animais destinados ao abate, bem como evitar a contaminação cruzada nos estabelecimentos manipuladores de alimentos. Palavras-chave:Método de diagnóstico; Porco; Reação em cadeia da polimerase; Bactéria. AbstractThe production of pork has been increasing in recent years as well as the requirement for quality products, free of any hazard for consumers. Salmonella spp. represents a biological hazard and has been identified worldwide as the agent responsible for many cases of foodborne diseases. Swine are considered to be an important reservoir of Salmonella spp. and hence knowledge of the dynamics of the bacteria throughout the pork production chain is essential. The objective of this study was to determine the prevalence of Salmonella spp. in pigs slaughtered in the Federal District of Brazil. A total of 240 samples of mesenteric lymph nodes (LM) were collected and also 240 carcass swabs (SC), in a slaughterhouse submitted to official inspection. The samples were subjected to the PCR (Polymerase Chain Reaction) assay for bacterial detection, revealing a prevalence of 68.75% in the mesenteric lymph nodes of the slaughtered pigs and 29.17% in the SC. Moreover, the data analysis rev...

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“…Total DNA was isolated by boiling lysis (Santos et al, 2001). A 400μL sample was placed in a 1.5mL polypropylene tube, free from DNA and RNA (Axygen).…”

Section: Salmonella Sppmentioning

confidence: 99%

Detection of Salmonella spp. by Conventional Bacteriology and by Quantitative Polymerase-Chain Reaction in Commercial Egg Structures

Moraes

Duarte

Bastos

et al. 2016

Rev. Bras. Cienc. Avic.

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Conventional bacteriology techniques and quantitative polymerasechain reaction (qPCR) were applied to the eggshell, albumen, and yolk of washed and unwashed commercial white and brown eggs, to detect Salmonella spp. Pooled samples of eggshells, albumen, and yolk of white and brown eggs were collected at the poultry house and at the egg-storage room. Salmonella spp. was detected by conventional bacteriology in 5.4% (21/387) of analyzed samples and in 16% (68/387) by qPCR. In the 114 unwashed white eggs samples of eggshell, albumen and yolk, the bacterium was identified in 2.6% of the eggs (3/114) by conventional bacteriology and in 13.2% (15/114) by qPCR. In the 90 samples of washed eggs, 6.7% (6/90) were contaminated as detected by conventional bacteriology and 10.0% (9/90) by qPCR. In the 81 samples of unwashed brown eggs, Salmonella spp. was detected in 6.1% of the eggs (5/81) by conventional bacteriology and 27.2% (22/81) by qPCR. In the 102 samples of brown washed eggs, 6.9% (7/102) where positive by conventional bacteriology and 35.3% (16/102) by qPCR. All samples detected as positive by conventional bacteriology were also positive by qPCR. Salmonella Agona represented 18.2% (4/22) of identified serovars, Salmonella enterica subs. enterica O: 4.5 18.2%

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Polymerase chain reaction (PCR) for the detection of Salmonella in artificially inoculated chicken meat

Cited by 24 publications

References 6 publications

Quick identification and quantification of Proteus mirabilis by polymerase chain reaction (PCR) assays

Quick identification and quantification of Proteus mirabilis by polymerase chain reaction (PCR) assays

Prevalência de Salmonella spp. em suínos abatidos em um frigorífico do Distrito Federal determinada pela técnica de PCR

Detection of Salmonella spp. by Conventional Bacteriology and by Quantitative Polymerase-Chain Reaction in Commercial Egg Structures

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