Production of monoclonal antibodies anti-Taenia crassiceps cysticerci with cross-reactivity with Taenia solium antigens

Espíndola, Noeli Maria; Gaspari, Elizabeth De; Nakamura, Paulo Mutuko; Vaz, Adelaide José

doi:10.1590/s0036-46652000000300013

Cited by 17 publications

(7 citation statements)

References 9 publications

(10 reference statements)

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“…When the gp-Tcra antigen was used, however, only the 18-and 14-kDa peptides, which are specific for the diagnosis of NC (1,5), were identified, thus facilitating the interpretation of the results. The reactivity against low-molecular-mass peptides (Յ20 kDa) found in T. crassiceps antigen extracts by immunoblotting was also observed in T. solium antigen extracts (22).…”

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confidence: 99%

Use of Taenia crassiceps Cysticercus Antigen Preparations for Detection of Antibodies in Cerebrospinal Fluid Samples from Patients with Neurocysticercosis ( Taenia solium )

Pardini

Peralta

Vaz³

et al. 2002

Clin Vaccine Immunol

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Antigen extracts obtained from the vesicular fluid of Taenia crassiceps cysticerci and from fractions purified by affinity chromatography with the lectin concanavalin A and the glycoprotein antigen separated by electrophoresis were used for the detection of Taenia solium anticysticercus antibodies. The sensitivity and specificity obtained for all antigens were 100% in enzyme-linked immunosorbent assay with good reproducibility. Using immunoblotting of the three antigens, low-molecular-mass peptides (18 and 14 kDa) were characterized only in cerebrospinal fluid samples from patients with neurocysticercosis. The results confirm that antigen fractions purified from T. crassiceps cisticerci are important sources of specific peptides and proved to be efficient in detecting anti-T. solium antibodies.Neurocysticercosis (NC), caused by the larval form of Taenia solium, is considered to be an endemic disease in underprivileged regions such as Latin America, Asia, Africa, China, and Indonesia (2,16,23,26), and it represents an important public health problem in these regions.The diagnosis of NC is based on clinical and laboratory criteria and on imaging examinations such as axial computed tomography and magnetic resonance imaging, which are efficient methods for the visualization of cysticerci and of an inflammatory response but are very expensive and inaccessible to most of the affected population (4).Although the immunological tests used for the diagnosis of cysticercosis have been considerably refined, the selection of an adequate antigen is required to improve diagnostic efficiency, and this continues to be an area of interest.Earlier studies have demonstrated the importance of the use of purified extracts obtained mainly from the glycoprotein fractions of T. solium for the detection of antibodies against cysticercus antigens in immunological assays (6,7,10,11,22). The difficulty of obtaining parasites from naturally infected pigs for the isolation of T. solium antigen continues to be a problem. Therefore, our group has been studying the use of antigens obtained from cysticerci of the Taenia crassiceps ORF strain (1, 25). The objective of the present study was to determine the efficiency of antigen fractions purified from T. crassiceps cysticerci in the detection of specific antibodies in cerebrospinal fluid (CSF) samples from patients with NC.Parasites and antigens. Vesicular fluid antigen was obtained from the larval form of T. crassiceps (VF-Tcra) ORF strain (8) as described previously (25). The purified fractions of concanavalin A (ConA-Tcra) were obtained by affinity chromatography with lectin. Total antigen was subjected to gel filtration on a PD-10 Sephadex G-25M column, purified by affinity chromatography on a ConA-Sepharose 4B column (Pharmacia Fine Chemicals) equilibrated with Tris-HCl buffer (pH 7.4) (0.5 M NaCl, 0.05 M Tris), and eluted with 0.2 M ␣-methylmannopyranoside solution. The protein peak was detected by spectrophotometry at 280 nm and concentrated by ultrafiltration through a YM-10 membrane (Amic...

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confidence: 99%

Use of Taenia crassiceps Cysticercus Antigen Preparations for Detection of Antibodies in Cerebrospinal Fluid Samples from Patients with Neurocysticercosis ( Taenia solium )

Pardini

Peralta

Vaz³

et al. 2002

Clin Vaccine Immunol

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“…However, Furrows et al(2006) showed there was an heterogeneous reactivity in NCC immunodiagnosis and, for this, single band findings in an EITB must be interpreted with special caution. Espíndola (2000) observed that hyperimmune mouse BALB/c serum against crude antigen of T. crassiceps was able to recognize peptides of 18 and 12-14 kDa of T. crassiceps. In the same experiment, it was observed that the 15, 70, and 94 kDa antigens of T. solium were not specific, since they reacted with serum from negative control animals.…”

Section: Discussionmentioning

confidence: 96%

Crude antigen from Taenia crassiceps cysticercus used as heterologous antigen in ELISA and in EITB for neurocysticercosis diagnosis of patients from Paraná-Brazil

Minozzo

Moura

Almeida

et al. 2008

Braz. arch. biol. technol.

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“…Monoclonal antibodies against T. crassiceps (17) and T. saginata (18) have been developed, as well as against T. solium eggs (19), to be used in the diagnosis of NCC. Our approach was to develop TsmAbs to various components and products of T. solium cysts, whole antigen (WA), excretion/secretion products (ES) and vesicular fluid (VF).…”

Section: Discussionmentioning

confidence: 99%

Anti-Taenia solium monoclonal antibodies for the detection of parasite antigens in body fluids from patients with neurocysticercosis

Paredes

Sáenz

Marzal

et al. 2016

Experimental Parasitology

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Neurocysticercosis (NCC), an infection of the brain by Taenia solium (Ts) cysts, is the most common cause of adult-onset epilepsy in developing countries. Serological testing consists primarily of varying methods to detect antibodies in body fluids and more recently antigen (Ag) detection assays to identify individuals or animals with viable parasites. Antigen assays currently in use employ monoclonal antibodies (mAbs) raised against T. saginata, which have known cross reactivity to animal cestodes but are highly specific in human samples. We produced, characterized and tested 21 mAbs raised against T. solium whole cyst antigens, vesicular fluid or excretory secretory products. Reactivity of the TsmAbs against specific cyst structures was determined using immunofluorescence and immunohistochemistry on histological sections of Ts muscle cysts. Four TsmAbs reacted to vesicular space alone, 9 to the neck and cyst wall, one to the neck and vesicular space and 7 to the neck, cyst wall and vesicular space. An in-house ELISA assay to detect circulating Ts antigen, using the TsmAbs as capture antibodies and a rabbit polyclonal anti-Ts whole cyst antibody as a detector antibody demonstrated that eight of the 21 TsmAbs detected antigens in known NCC-positive human sera and three of these also in urine samples. Reactivity was expressed as normalized ratios of optical densities (OD positive control/OD negative control). Three TsmAbs had ratios >10 and five between 2–10. The TsmAbs have potential utility for the diagnosis and post-treatment monitoring of patients with viable NCC infections.

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Production of monoclonal antibodies anti-Taenia crassiceps cysticerci with cross-reactivity with Taenia solium antigens

Cited by 17 publications

References 9 publications

Use of Taenia crassiceps Cysticercus Antigen Preparations for Detection of Antibodies in Cerebrospinal Fluid Samples from Patients with Neurocysticercosis ( Taenia solium )

Use of Taenia crassiceps Cysticercus Antigen Preparations for Detection of Antibodies in Cerebrospinal Fluid Samples from Patients with Neurocysticercosis ( Taenia solium )

Crude antigen from Taenia crassiceps cysticercus used as heterologous antigen in ELISA and in EITB for neurocysticercosis diagnosis of patients from Paraná-Brazil

Anti-Taenia solium monoclonal antibodies for the detection of parasite antigens in body fluids from patients with neurocysticercosis

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