Anti-phenolic glycolipid-I (PGL-I) determination using blood collection on filter paper in leprosy patients

Tomimori‐Yamashita, Jane; Nguyen, Thu Huong; Maeda, Solange M.; Flageul, B.; Rotta, Osmar; Cruaud, P.

doi:10.1590/s0036-46651999000400007

Cited by 7 publications

(3 citation statements)

References 13 publications

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“…A close correlation was observed between studies using samples in filter paper (finger prick) 25 42 67 69 and those collected by venopunction, despite the fact that the serological titers detected on samples that passed through filter paper were generally lower 74 .…”

Section: Discussionmentioning

confidence: 68%

Sorologia da hanseníase utilizando PGL-I: revisão sistemática

Moura

Calado

Oliveira

et al. 2008

Rev. Soc. Bras. Med. Trop.

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Serology using a species-specific antigen for Mycobacterium leprae, PGL-I, could be a marker for the bacterial load of patients with leprosy. Various studies have identified the potential use of serology in the classification of patients for treatment purposes, case monitoring, identification of the risk of relapse and selection of household contacts with a higher risk of contracting the disease. A systematic review of the literature was conducted and 26 articles were included in this comparative analysis. The results of the use of PGL-I serology in different situations, its limitations and possible applications were evaluated. Studies show the efficacy of PGL-I serology in the classification of patients, treatment monitoring and as a predictive test for leprosy reactions. To improve early diagnosis and follow-up of the population at greatest risk of developing leprosy, the methodologies used in the past have yet to show a favorable cost-benefit ratio, although studies indicate that the use of the test might positively influence leprosy control programs. With simple and robust techniques, the use of PGL-I serology is viable.

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Section: Discussionmentioning

confidence: 68%

Sorologia da hanseníase utilizando PGL-I: revisão sistemática

Moura

Calado

Oliveira

et al. 2008

Rev. Soc. Bras. Med. Trop.

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“…Filter paper has been considered to be used for diagnosis of new cases in leprosy. A study showed that there is a straight correlation between antibody anti-PGL-1 test using a blood sample from filter paper and classical method [ 12 ]. However, another study in Indonesia found that there are no significant differences between the examination of antibody anti-PGL-1 level using filter paper and conventional method [ 13 ].…”

Section: Discussionmentioning

confidence: 99%

“…A study has been done in detecting IgM antibody against PGL-1 by using filter paper with a finger prick method using ELISA test [ 12 ]. While in Indonesia, a study was conducted to compare examination of antibody anti-PGL-1 level in blood samples from leprosy patient with and without using filter paper.…”

Section: Introductionmentioning

confidence: 99%

IgM Anti PGL-1 Antibody Level in Patients with Leprosy: A Comparative Study between Ear Lobes Capillary and Median Cubital Vein Blood Samples

Nasution¹,

Nadeak²,

Lubis³

2018

Open Access Maced J Med Sci

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BACKGROUND:To establish the diagnosis of leprosy accurately, additional examination such as serologic examination with ELISA is required. There are considerations about taking a blood sample from the earlobe region.AIM:To determine the differences in IgM anti-PGL-1 antibody levels from earlobe capillary and median cubital vein blood sample in leprosy patients.METHODS:An observational analytic study using a cross-sectional study involving 30 patients with leprosy. ELISA examination of earlobe blood samples with filter paper, and the median cubital vein blood samples with filter paper and conventional methods were performed to determine IgM anti-PGL-1 antibody levels.RESULTS:The mean value of IgM anti PGL-1 antibody levels from earlobe blood samples with filter paper (1476.62 μ/ml) was relatively similar with median cubital vein blood samples with conventional method (1476.77 μ/ml), but the mean value of IgM anti PGL-1 antibody levels from median cubital vein blood samples with filter paper (1210.37 μ/ml) was lower from other methods. However, there was no statistically significant difference between them.CONCLUSION:There are no significant differences between the mean levels of IgM anti-PGL-1 antibody from earlobe and the median cubital vein blood samples.

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Leprosy

Haimanot

Melaku

2000

Current Opinion in Neurology

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Leprosy is a unique infectious disease with a prolonged incubation period and a predilection for skin and nerves. The involvement of nerves by the primary infection as well as the immunologically mediated reversal reactions result in impairment of nerve function and severe disabilities. The introduction of the World Health Organization Multi Drug Therapy over the last two decades has produced dramatic changes in the management and control programmes of leprosy. A recent important contribution to the understanding of leprosy pathogenesis has been the elucidation of the molecular basis for the entry of Mycobacterium leprae into the Schwann cell and the peripheral nerve. Leprosy still remains the commonest cause of peripheral neuropathy in developing countries.

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Anti-phenolic glycolipid-I (PGL-I) determination using blood collection on filter paper in leprosy patients

Cited by 7 publications

References 13 publications

Sorologia da hanseníase utilizando PGL-I: revisão sistemática

Sorologia da hanseníase utilizando PGL-I: revisão sistemática

IgM Anti PGL-1 Antibody Level in Patients with Leprosy: A Comparative Study between Ear Lobes Capillary and Median Cubital Vein Blood Samples

Leprosy

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