Dot-ELISA for the detection of anti-Cysticercus cellulosae antibodies in cerebrospinal fluid using a new solid phase (resin-treated polyester fabric) and Cysticercus longicollis antigens

Abstract

A dot-ELISA was developed for the detection of antibodies in CSF in the immunologic diagnosis of human neurocysticercosis, using antigen extracts of the membrane and scolex of Cysticercus cellulosae (M+S-Cc) and, alternately, membrane (M) and vesicular fluid (VF) of Cysticercus longicollis (Cl) covalently bound to a new solid phase consisting of polyester fabric treated with N-methylol-acrylamide resin (dot-RT). The test was performed at room temperature, with reduced incubation times and with no need for special care in the manipulation of the support. The sensitivity rates obtained were 95.1% for antigen Cc and 97.6% for antigen Cl. Specificity was 90.6% when Cc was used, and 96.9% and 100% when M-Cl and VF-Cl were used, respectively. No significant differences in titer were observed between tests carried out with homologous and heterologous antigens. The low cost and easy execution of the dot-RT test using antigen extracts of Cysticercus longicollis indicate the test for use in the immunodiagnosis of human neurocysticercosis.

Utility of cysticercus fasciolaris antigen in Dot ELISA for the diagnosis of neurocysticercosis

“…Dot ELISA has become increasingly popular for the diagnosis of many other parasitic diseases also, such as fi lariasis, [8] malaria, [9] and schistosomiasis, [10] besides cysticercosis. Dot ELISA has been used earlier for diagnosis of cysticercosis using antigens derived from cysticercus cellulosae, [11][12][13] cysticercus longicollis, [14] and T. solium glycoproteins. [15] Liu et al, 1996, have modifi ed the Dot ELISA by Dot-immunogold silver staining for diagnosis of cysticercosis.…”

“…[11] It is reported that sensitivity and specifi city of Dot ELISA generally vary from 56.25% to 97.61% and from 90.6% to 92% respectively. [11,12,14] The difference can be explained by different criteria for patient selection and different gold standards used in different studies. We have used visibility of scolex in scans, an absolute diagnostic criterion, which also denotes active lesions; and hence seropositivity was high despite presence of a single cyst in several cases.…”

Relationship between Serum Antibodies and Taenia solium Larvae Burden in Pigs Raised in Field Conditions

“…Immunological assays appear to be best suited for field surveys as pigs can be bled rapidly from the anterior cava vein and it is less dangerous for the examiner than examination of the tongue [9]. A number of assays have been developed for the detection of antibodies, including Enzyme Linked Immuno Sorbent Assay (ELISA) tests with secretory/excretory antigens [10] and fluid antigens [11], and indirect ELISA using heterologous antigens from T. crassiceps [12], [13] and Cysticercus longicolis [14].…”

Crude antigen from Taenia crassiceps cysticercus used as heterologous antigen in ELISA and in EITB for neurocysticercosis diagnosis of patients from Paraná-Brazil

“…However, it is very difficult to obtain enough quantities of an antigen as that depends either on finding naturally infected swine or infecting them experimentally with T. solium eggs. Several studies have shown that C. longicollis could replace C. cellulosae because both parasites shared antigenic elements able to be used in ELISA or immunoblottings (Vaz et al, 1996;Bueno et al, 2000;Pardini et al, 2002;Ishida et al, 2006). Therefore, the use of this heterologous antigen may contribute for seroepidemiologic studies to evaluate the complex taeniasis/cysticercosis (Vaz et al, 1996;, Sciutto et al, 2000, Ito, 2000.…”

“…Several studies have shown that C. longicollis could replace C. cellulosae because both parasites shared antigenic elements able to be used in ELISA or immunoblottings (Vaz et al, 1996;Bueno et al, 2000;Pardini et al, 2002;Ishida et al, 2006). Therefore, the use of this heterologous antigen may contribute for seroepidemiologic studies to evaluate the complex taeniasis/cysticercosis (Vaz et al, 1996;, Sciutto et al, 2000, Ito, 2000. Furthermore, studies have shown that immunoassays were able to detect whether the patients presented alive or inactive cysticerci (Molinari et al, 2002, Barcelos et al, 2005.…”