Immunoperoxidase for the detection for the detection of antibodies in cerebrospinal fluid in neurocysticercosis: use of Cysticercus cellulosae and Cysticercus longicollis particles fixed on microscopy slides

Abstract

The ORF strain of Cysticercus longicollis represents an important model for the study of heterologous antigens in the immunodiagnosis of neurocysticcreosis (NC). The immunoperoxidase (IP) technique was standardized using a particulate antigen suspension of Cysticercus longicollis (Cl) and Cysitcercus cellulosae (Cc). Cerebrospinal fluid (CSF) samples were incubated on the antigen fixed to microscopy slides; the conjugate employed was anti-IgG-peroxidase and the enzymatic reaction was started by covering the slides with chromogen solution (diaminobenzidine/H2O2). After washing with distilled water, the slide was stained with 2% malachite green in water. Of the CSF samples from 21 patients with NC, 19 (90.5%) were positive, whereas the 8 CSF samples from the control group (100%) were negative. The results of the [P-C] test applied to 127 CSF samples from patients with suspected NC showed 28.3% reactivity as opposed to 29.1% for the IP-Cc test. The agreement index for the IP test (Cl x Cc) was 94.2%, with no significant difference between the two antigens.

Neurocysticercosis: detection of IgG, IgA and IgE antibodies in cerebrospinal fluid, serum and saliva samples by ELISA with Taenia solium and Taenia crassiceps antigens

“…One of the difficulties in such studies is obtaining parasites from the model of natural infection in swine. Taenia crassiceps metacestodes, found in rodents, have been described in natural and experimental infections, and have been used as an alternative source of antigen in immunological tests for NC 2,[7][8][9][10][11] .…”

Total IgE detection in paired cerebrospinal fluid and serum samples from patients with neurocysticercosis

“…The diagnosis of NC is based on clinical, epidemic and laboratory data (neuroimaging and immunological methods). Since clinical diagnosis is hindered by the nonspecific and polymorphic symptomatology of NC, the detection of anti-T. solium antibodies in cerebrospinal fluid (CSF) represents an important diagnostic factor 2,8,9,13,22,23 .…”

Anti-Taenia solium metacestodes antibodies in serum from blood donors from four cities of Triângulo Mineiro area, Minas Gerais, Brazil, 1995

“…ELISA and EITB together may supply reliable results for the diagnosis of human cysticercosis 3,22 , but EITB is too costly for application to populations of developing countries. Reports of the literature have reinforced the necessity in developing more reliable immunological methods to detect antibodies anti-metacestodes in serum or cerebrospinal fluid 1,4,7,16,17 .…”