Vibrio metschnikovii among diarrheal patients during cholera epidemic in Recife Brazil

Magalhäes, Vera; Branco, Adeíza; Lima, Roberto de Andrade; Magalhães, Marcelo

doi:10.1590/s0036-46651996000100001

Cited by 17 publications

(9 citation statements)

References 6 publications

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“…even though the oxidase test was negative. Consequently our results are in agreement with those from the literature that demonstrate that typical colony morphology is an important step to assure isolation of V. metschnikovii (Dalsgaard et al, 1996;Elhadi et al, 2004;Farmer, Hickman-Brenner, Fanning, Gordon, & Brenner, 1988;Magalhães et al, 1996;Urdaci et al, 1988).…”

Section: Discussionsupporting

confidence: 92%

“…As demonstrated previously by Magalhães et al (1996), because an active search for vibrios was undertaken, it was possible to detect V. metschnikovii in the samples examined. These authors reported 6 isolates of V. metschnikovii from diarrheal patients in Brazil during their surveillance for cholera in the same period as V. metschnikovii strains from the present study were obtained.…”

Section: Discussionmentioning

confidence: 57%

“…In Brazil the first evidence on V. metschnikovii producing gastroenteritis in health individuals was reported by Magalhães, Branco, Lima, and Magalhães (1996), when this specie was isolated from the stool of 6 diarrheal patients in Recife.…”

Section: Introductionmentioning

confidence: 99%

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Virulence factors of Vibrio metschnikovii strains isolated from fish in Brazil

et al. 2007

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 57%

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Virulence factors of Vibrio metschnikovii strains isolated from fish in Brazil

et al. 2007

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“…This organism is one of the species of Vibrio that can be found in human clinical specimens. Although not a bona fide human pathogen, V. metschnikovii has been reported to cause human diarrhea (Dalsgaard et al, 1996;Buck, 1992;Magalhaes et al, 1996). Furthermore, V. metschnikovii has been isolated from lakes and streams with evidence of fecal contamination (Dalsgaard et al, 1996;Bitto et al, 1992).…”

Section: Analysis Of Bacterial Dna From the Iceman's Gastrointestinalmentioning

confidence: 99%

Sequence analysis of bacterial DNA in the colon and stomach of the Tyrolean Iceman

Cano

Tiefenbrunner

Ubaldi

et al. 2000

Am. J. Phys. Anthropol.

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The male human body found in an Alpine glacier on September 19, 1991 ("Tyrolean Iceman") has, for the first time in history, given scientists a chance to perform detailed anatomical, histological, and molecular investigations on the organs of a person from the Neolithic Age (5350-5100 B.P.). In the present study, tissue samples aseptically taken from the stomach and the colon of the mummy were utilized for DNA extraction, and the DNA was PCR-amplified, using primer pairs designed to bind to fragments of the 16s ribosomal RNA gene (16s rDNA) of a broad range of bacteria. The PCR products were cloned in plasmid vectors, and the recombinant clones (amplicons) were sequenced. The sequence data were finally used for scanning data libraries containing the corresponding sequences of present-day bacteria, to infer the putative ecophysiology of the ancient ones. The same procedure was repeated on some fragments of grass from the clothing found near the corpse. These fragments were taken as a control of the microbiological situation of the glacier. The results show that the flora of the Iceman's stomach is entirely composed of Burkholderia pickettii, an organism commonly found in aquatic habitats. The colon, on the other hand, contains several members of the fecal flora of humans, such as Clostridium perfringens, C. ghonii, C. sordellii, Eubacterium tenue, and Bacteroides sp. The Iceman's colon, however, was found to contain, rather unexpectedly, also some members of the genus Vibrio. The results are discussed in light of what is known about the preservation of microbial DNA at the Iceman's site and of previous parasitological studies performed on the Iceman himself and on human coprolites.

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“…This facultative bacterium is often found in natural aquatic environments such as seawater (9). In addition, the bacterium has been known as one of causative agents that can induce dyspneic symptoms (10), diarrhea (11,12), wound infection (13), pneumonia (1) and leg ulcer (9). There have been brief reports on alkaline proteases (AprB, AprJ1, VapT and VapK) from a few V. metschnikovii strains.…”

Section: Introductionmentioning

confidence: 99%

Purification and characterization of a novel alkaline serine protease secreted by Vibrio metschnikovii

Park

Park²,

Park³

et al. 2011

Int J Mol Med

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Abstract.A novel extracellular alkaline serine protease secreted by Vibrio metschnikovii (V. metschnikovii) ATCC700040 cells was purified by three chromatographic steps and characterized in terms of enzymatic kinetics and substrate specificity. The purified enzyme (named AKP-Vm) was composed of a single polypeptide with an apparent molecular weight of 50 kDa on 12% SDS-polyacrylamide gel in the presence of CuCl 2 . The optimal temperature and the pH for the enzyme were found to be 37˚C and 9.5, respectively. However, the enzyme activity was inhibited by inhibitors such as PMSF and aprotinin. AKP-Vm could hydrolyze a peptide bond at the carboxyl side of the arginine residue, as revealed by its amidolytic activity toward a chromogenic substrate, Boc-Val-Pro-Arg-pNA. . AKP-Vm protease could cleave various blood coagulation-associated proteins, including fibrinogen, prothrombin and thrombin. In particular, the enzyme showed powerful fibrinogenolytic and fibrinolytic activities, as it could cleave all major chains of fibrinogen and also digest cross-linked fibrin. The results obtained suggest that AKP-Vm is a novel alkaline serine protease that can actively cleave fibrinogen and cross-linked fibrin.

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Vibrio metschnikovii among diarrheal patients during cholera epidemic in Recife Brazil

Cited by 17 publications

References 6 publications

Virulence factors of Vibrio metschnikovii strains isolated from fish in Brazil

Virulence factors of Vibrio metschnikovii strains isolated from fish in Brazil

Sequence analysis of bacterial DNA in the colon and stomach of the Tyrolean Iceman

Purification and characterization of a novel alkaline serine protease secreted by Vibrio metschnikovii

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