Serological findings during a measles outbreak occurring in a population with vaccine coverage

Oliveira, Solange Artimos de; Siqueira, Marilda M.; Costa, Antônio José Leal; Almeida, Maria T.C.; Nascimento, Jussara Pereira do

doi:10.1590/s0036-46651995000500007

Cited by 5 publications

(2 citation statements)

References 19 publications

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“…From the 92 cases received from ES in the years of 1992 and 1994, we had the same number of positives for measles and B19. In a previous study of an outbreak of exanthem in Niterói, RJ during 1991 we obtained 80% of laboratory confirmation of 112 measles reported cases 19 . In that study no cases with anti-B19 IgM were found and rubella was diagnosed in only four cases.…”

Section: Discussionmentioning

confidence: 85%

Human parvovirus B19 infections among exanthematic diseases notified as measles

Cubel

Siqueira²,

Santos³

et al. 1997

Rev. Soc. Bras. Med. Trop.

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A total of 1397 sera collected from 1095 cases of exanthematic disease notified as measles in ES and RJ states during July 1992 to December 1994 were investigated. These sera were first tested for measles and rubella specific IgM. When they proved negative, they were tested for B19 specific IgM by an enzyme immunoassay. B19 infection was confirmed in 27 (2.5%) of these cases. Sera from 194 negative cases for measles and rubella IgM received from other Brazilian states were also investigated and B19 infection was confirmed for 11 of them. Sera from these 38 IgM positive cases for B19, were tested for anti-B19 IgG by an enzyme immunoassay and for B19 DNA by dot blot hybridization. Anti-B19 IgG antibodies were detected in most of the acute sera. B19 DNA was detected in the acute serum of one patient that had been splenectomized before. As the exanthem caused by human parvovirus infection may be clinically diagnosed as rubella, it could be important to diagnose B19 infection in Brazil since it is becoming prevalent as the cause of rash in countries where rubella is controlled by vaccination.
Um total de 1397 soros colhidos de 1095 casos de exantema notificados como sarampo, nos estados do ES e RJ, no período de julho de 1992 a dezembro de 1994, foram investigados. Estes soros foram inicialmente testados para sarampo e rubéola por detecção de IgM específica. Os casos negativos foram então testados para a presença de IgM específca anti-B19 por um ensaio imunoenzimático. A infecção pelo B19 foi confirmada em 27 (2,5%) destes casos. Soros de 194 casos negativos para sarampo e rubéola provenientes de outros estados brasileiros foram também investigados, e a infecção pelo B19 pode ser confirmada em 11 destes casos. Os soros dos 38 casos IgM positivos para B19 foram testados para a presença de IgG específica anti-B19 por um ensaio imunoenzimático, e para a presença do ADN viral por hibridização em "dots" (dot blot). IgG específica anti-B19 pode ser detectada na maioria dos soros de fase aguda, e o ADN viral foi detectado no soro de fase aguda de um paciente esplenectomizado. Como o exantema causado pela infecção pelo parvovírus humano pode ser clinicamente diagnosticado como rubéola, seria importante realizar o diagnóstico desta virose no Brasil, já que um aumento no número de casos de exantema por B19 tem sido relatado nos países onde a rubéola é controlada por vacinação

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Section: Discussionmentioning

confidence: 85%

Human parvovirus B19 infections among exanthematic diseases notified as measles

Cubel

Siqueira²,

Santos³

et al. 1997

Rev. Soc. Bras. Med. Trop.

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“…The former could be related to the higher proportion of virus antibody negative indi-viduals in younger age groups. The later resulted probably from the timing of specimen collection, since test sensitivity is known to be lower in the early period of the disease, when antibody levels are not high enough to be detected [12,29,30].…”

Section: Discussionmentioning

confidence: 99%

The aetiology of maculopapular rash diseases in Niterói, State of Rio de Janeiro, Brazil: implications for measles surveillance

et al. 2001

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A study investigating the causes of rash diseases using systematic laboratory testing was conducted in Niterói, Rio de Janeiro, between January 1994 to April 1998. Sera from 327 patients were tested for evidence of anti-rubella virus, measles virus, human parvovirus B19 and dengue fever virus specific immunoglobulin IgM and anti-human herpes virus type 6 (HHV-6) IgG antibodies. A laboratory confirmed diagnosis was achieved in 71.3% of the cases investigated: dengue fever (33.0%), rubella (20.2%), parvovirus B19 (9.2%), measles (6.7%) and HHV-6 (2.1%). No diagnosis was established for 94 cases (28.7%). An outbreak of measles was detected during 1997, with a peak in September and October. All of the diseases studied here presented with clinical features similar to measles and classical symptoms were found in all measles confirmed cases. The large overlap of combinations of signs and symptoms seen in this study highlights the difficulties of diagnosing a rash illness on clinical grounds alone.

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Salivary diagnosis of measles for surveillance: a clinic-based study in Niterói, State of Rio de Janeiro, Brazil

Oliveira

Siqueira

Brown

et al. 1998

Transactions of the Royal Society of Tropical Medicine and Hygi

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This study was designed to investigate whether saliva could be a feasible alternative to serum for the diagnosis of recent measles infection in a clinic setting. Forty-two paired blood and saliva samples collected 1 to 16 days after onset of illness from 29 patients with clinical measles were tested for specific immunoglobulin (Ig) M by antibody-capture radioimmunoassay. Measles IgM was detected in all serum samples and in 39 (92.9%) saliva specimens. Between 1 and 3 weeks after illness onset, virus-specific IgM was detected in 100% of saliva samples. Measles IgM was also detected in 17 saliva specimens, not paired with blood samples, collected from study patients 5 days to 3 weeks after onset. Our results indicate that salivary IgM detection is a suitable non-invasive method for investigation of notified cases under conditions of routine clinic use.

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Serological findings during a measles outbreak occurring in a population with vaccine coverage

Cited by 5 publications

References 19 publications

Human parvovirus B19 infections among exanthematic diseases notified as measles

Human parvovirus B19 infections among exanthematic diseases notified as measles

The aetiology of maculopapular rash diseases in Niterói, State of Rio de Janeiro, Brazil: implications for measles surveillance

Salivary diagnosis of measles for surveillance: a clinic-based study in Niterói, State of Rio de Janeiro, Brazil

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