Murine virus contaminant of Trypanosoma cruzi experimental infection

Rangel, Humberto de Araújo; Verinaud, Liana; Camargo, I. J. B.; Gilioli, Rovílson; Sakurada, Júlia Keiko

doi:10.1590/s0036-46651994000500006

Cited by 4 publications

(4 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our results on the aggravation of T. cruzi infection in BALB/c and C57BL/6 mice by using parasite inocula maintained in mice that had positive serology for MHV are in agreement with a previous report on CBA/J mice infected with T. cruzi derived from corona virus-positive mice. 35 , 36 These authors were able to demonstrate, in the plasma, infective virus particles that could be neutralized by anti-MHV antiserum. Nevertheless, the underlying mechanisms leading to increased susceptibility to the parasite were not explored.…”

Section: Discussionmentioning

confidence: 96%

Interference of natural mouse hepatitis virus infection with cytokine production and susceptibility to Trypanosoma cruzi

et al. 1999

View full text Add to dashboard Cite

Mouse hepatitis virus (MHV) infection can have a pronounced impact on several investigation areas. Reports on natural MHV outbreaks are rare and most studies have been conducted by deliberately infecting mice with MHV laboratory strains that cause moderate to severe disturbances to the immune system. We have investigated the effects of a natural acute outbreak of MHV in our otherwise specific-pathogen-free (SPF) inbred mouse colonies, and of enzootic chronic MHV infection on cytokine production and resistance to the intracellular pathogen Trypanosoma cruzi. We found that BALB/c and/or C57BL/6 SPF mice that had been injected with T. cruzi blood trypomastigotes from recently MHV-contaminated (MHV+) mice developed significantly higher parasite blood counts, accelerated death, and showed higher IL-10 production by spleen cells than their counterparts whose T. cruzi inoculum was derived from MHV-negative (MHV-) donors. Interferon-gamma (IFN-gamma) production by MHV+ and MHV- mice was not significantly different. In contrast, T. cruzi infection of chronically MHV-infected mice did not result in major changes in the course of infection when compared with that observed in mice from MHV- colonies, although a trend to higher parasitaemia levels was observed in BALB/c MHV+ mice. Nevertheless, both BALB/c and C57BL/6 T. cruzi-infected MHV+ mice had diminished IFN-gamma production to parasite-antigen stimulation in comparison with similarly infected MHV- mice. Interleukin-10 (IL-10) production levels by spleen cells did not differ between chronic MHV+ and MHV- mice, but IFN-gamma neutralization by monoclonal antibody treatment of anti-CD3-stimulated spleen cell cultures showed higher levels of IL-10 synthesis in MHV+ BALB/c mice.

show abstract

Section: Discussionmentioning

confidence: 96%

Interference of natural mouse hepatitis virus infection with cytokine production and susceptibility to Trypanosoma cruzi

et al. 1999

View full text Add to dashboard Cite

show abstract

“…Besides, results can be distorted and misunderstood by concurrent infections, as reported previously in a biological characterization of a T . cruzi strain [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

Quality of Reporting and Adherence to ARRIVE Guidelines in Animal Studies for Chagas Disease Preclinical Drug Research: A Systematic Review

2015

View full text Add to dashboard Cite

Publication of accurate and detailed descriptions of methods in research articles involving animals is essential for health scientists to accurately interpret published data, evaluate results and replicate findings. Inadequate reporting of key aspects of experimental design may reduce the impact of studies and could act as a barrier to translation of research findings. Reporting of animal use must be as comprehensive as possible in order to take advantage of every study and every animal used. Animal models are essential to understanding and assessing new chemotherapy candidates for Chagas disease pathology, a widespread parasitic disease with few treatment options currently available. A systematic review was carried out to compare ARRIVE guidelines recommendations with information provided in publications of preclinical studies for new anti-Trypanosoma cruzi compounds. A total of 83 publications were reviewed. Before ARRIVE guidelines, 69% of publications failed to report any macroenvironment information, compared to 57% after ARRIVE publication. Similar proportions were observed when evaluating reporting of microenvironmental information (56% vs. 61%). Also, before ARRIVE guidelines publication, only 13% of papers described animal gender, only 18% specified microbiological status and 13% reported randomized treatment assignment, among other essential information missing or incomplete. Unfortunately, publication of ARRIVE guidelines did not seem to enhance reporting quality, compared to papers appeared before ARRIVE publication. Our results suggest that there is a strong need for the scientific community to improve animal use description, animal models employed, transparent reporting and experiment design to facilitate its transfer and application to the affected human population. Full compliance with ARRIVE guidelines, or similar animal research reporting guidelines, would be an excellent start in this direction.

show abstract

“…Recently, we have shown that specific-pathogen-free CBA/J mice infected with the Y strain of T. cruzi, which is maintained by serial mouse passage of Y UEC stock, had higher levels of parasitemia and shorter survival times than animals infected with a stock of parasites (Y CT ) which was maintained exclusively in cell culture. Tests for virus-specific antibody using filtered plasma from Y UEC -infected animals indicated the presence in these animals of murine hepatitis virus type 3 (MHV-3) (20). The cultures in which Y CT is maintained are virus free, and thus, mice infected with this strain did not acquire the virus.…”

mentioning

confidence: 99%

Immunodepression Induced byTrypanosoma cruziand Mouse Hepatitis Virus Type 3 Is Associated with Thymus Apoptosis

Verinaud

Cruz‐Höfling²,

Sakurada

et al. 1998

Clin Diagn Lab Immunol

Self Cite

View full text Add to dashboard Cite

Trypanosoma cruzi-infected mice show disturbance in the peripheral immune system such as polyclonal lymphocyte activation, autoantibody production, and immunosuppression of T lymphocytes. Previous observations in our laboratory showed that some stocks ofT. cruzi can be contaminated with mouse hepatitis virus type 3 (MHV-3). Literature has shown that MHV-3 infection induces immunologic disorders characterized by thymic involution with marked cell depletion. However, the effects of interactions between MHV-3 and the parasite on the immune system are not well understood. In the present study specific-pathogen-free CBA mice were inoculated with MHV-3, alone or associated with different stocks of T. cruzi. Concurrent murine virus infection resulted in increased pathogenicity of T. cruzi infection shown by profound thymic atrophy; loss of cortical thymocytes; depletion of Thy1.2+, CD4+, and CD8+ cells; enhancement of in situ labeling of nuclear DNA fragmentation; and eventually, death of the animals. Such lines of evidence show that the mechanism underlying this thymic atrophy is associated with apoptosis. These results also suggest that MHV-3 can account for the increased immunosuppression observed during experimental infection with the parasite.

show abstract

Murine virus contaminant of Trypanosoma cruzi experimental infection

Cited by 4 publications

References 11 publications

Interference of natural mouse hepatitis virus infection with cytokine production and susceptibility to Trypanosoma cruzi

Interference of natural mouse hepatitis virus infection with cytokine production and susceptibility to Trypanosoma cruzi

Quality of Reporting and Adherence to ARRIVE Guidelines in Animal Studies for Chagas Disease Preclinical Drug Research: A Systematic Review

Immunodepression Induced byTrypanosoma cruziand Mouse Hepatitis Virus Type 3 Is Associated with Thymus Apoptosis

Contact Info

Product

Resources

About