Diferenças nas propriedades adesivas de Staphylococcus saprophyticus a células HEp-2 e eritrócitos

Abstract

S. saprophyticus é freqüentemente isolado de infecções do trato urinário de mulheres jovens e sexualmente ativas. Ao contrário de S. aureus, esta espécie não possui fatores de virulência bem definidos. O objetivo deste estudo é analisar a aderência de S. saprophyticus a células HEp-2 e eritrócitos de carneiro. As amostras foram isoladas a partir da urina de pacientes com infecção urinária. Foram realizados testes de hemaglutinação, aderência a células HEp-2 e a capacidade de carboidratos específicos inibirem as interações entre estes tipos celulares e S. saprophyticus. A maioria das cepas se mostrou hemaglutinante e sensível a inibição da hemaglutinação pela manose (100mM). Foram verificados altos níveis de aderência às células HEp-2. As diferenças em especificidade e nível de aderência do microrganismo a células de HEp-2 e eritrócitos sugerem a participação de diferentes adesinas nos processos de interações celulares.

Polyvalent Interactions in Biological Systems: Implications for Design and Use of Multivalent Ligands and Inhibitors

“…The interaction of a single Fc portion with its receptor seems to be too weak to [151] Prevotella intermedia periodontal infection P: lactoferrin-binding protein (lactoferrin inhibits the binding) P: fibronectin, collagen of type I and type IV, and laminin [152] Salmonella enteritidis severe diarrhea (common food poison from chicken and chicken eggs) P: fibronectin [153] Staphylococcus aureus major human pathogen responsible for wide range of infections, can proceed to meningitis, endocarditis, and osteomyelitis P: three repeats of a 38-residue D motif P: fibronectin-binding proteins (galel A and ZZ-FnBP B) P: protein (60 kDa) P: collagen-binding domain (CBD) of collagen adhesin P: collagen receptor containing either two or three copies of a repeat motif with 187 amino acids P: N-terminal fragment (29 kDa) of fibronectin (Fn29 K) [154] P: fibronectin [155] P: bone sialoprotein (BSP) (small, ca. 80 kDa) integrin-binding, RGD-containing bone matrix glycoprotein [156,157] P: collagen of cartilage [158] P: collagen [159,160] P: N-terminal region (heparin-binding domain) of fibronectin [161] Staphylococcus aureus osteomyelitis and infectious arthritis P: collagen adhesin P: fibrinogen receptor (clumping factor) fibronectin-binding protein (FnBP) P: unknown protein (60 kDa) P: collagen (high degree of specificity and affinity) [162] P: fibrinogen [163,164] P: laminin (the pepsin-derived (P1) fragment) [165,166] P: vitronectin [167] Staphylococcus saprophyticus urinary tract infections S: adherence is partially inhibited by mannose [168] Streptococcus wide range of gastrointestinal and respiratory infections P: collagen type I [160] Group A streptococci infective endocarditis, glomerulonephritis, and rheumatic fever P: lipoteichoic acid (LTA) and M protein (binding is inhibited by LTA) [169,170] P: glyceraldehyde-3-phosphate dehydrogenase (GAPDH) [171,172] P: protein F [173] P: PAM (related to the M proteins) P: M3 protein [174] P: fibronectin P: human plasminogen and plasmin P: multiple binding to plasma fibrinogen, albumin and fibronectin Nephritis() and nephritis(-) group A streptococci glomerulonephritis P: nephritis plasmin binding protein (NPBP) [175] P:...…”

The hemagglutinin of Staphylococcus saprophyticus is a major adhesin for uroepithelial cells

“…In vitro, adhesive properties are normally assessed with permanent cell lines not originating from the human urinary tract. Strains isolated from clinical infections may differ grossly in their adhesive properties when tested in these assays, and nonadherent strains are not unusual (5,13). These variations may partly be a result of differences in membrane compositions between in vitro-grown cells and uroepithelial cells.…”