The hemagglutinin of Staphylococcus saprophyticus is a major adhesin for uroepithelial cells

Meyer, Hannelore; Wengler-Becker, U.; Gatermann, Sören

doi:10.1128/iai.64.9.3893-3896.1996

Cited by 31 publications

(7 citation statements)

References 16 publications

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“…The homologous autolysin/adhesins from other coagulase‐negative staphylococcal species, such as Aas from S. saprophyticus and AtlC from Staphylococcus caprae have been shown to bind to Fn (Hell et al ., 1998; Allignet et al ., 2001). Aas also binds to human uroepithelia and agglutinates sheep erythrocytes (Meyer et al ., 1996). Recently, the Atl homologues of further coagulase‐negative staphylococci have been characterized for their bacteriolytic activities, such as the AtlL of Staphylococcus lugdunensis (Bourgeois et al ., 2009) and Atl WM of Staphylococcus warneri M (Yokoi et al ., 2008).…”

Section: Introductionmentioning

confidence: 99%

A novel staphylococcal internalization mechanism involves the major autolysin Atl and heat shock cognate protein Hsc70 as host cell receptor

Nina

Schlesier²,

Schmidt

et al. 2010

Cellular Microbiology

132

125

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SummaryStaphylococcus aureus and Staphylococcus epidermidis can cause serious chronic and recurrent infections that are difficult to eradicate. An important pathogenicity factor in these infections caused by S. aureus is its ability to be internalized by non-professional phagocytes thereby evading the host immune system and antibiotic treatment. Here, we report a novel mechanism involved in staphylococcal internalization by host cells, which is mediated by the major autolysin/adhesins Atl and AtlE from S. aureus and S. epidermidis respectively. In a flow cytometric internalization assay, atl and atlE mutants are significantly reduced in their capacities to be internalized by endothelial cells. Moreover, pre-incubation of endothelial cells with recombinant Atl dose-dependently inhibited internalization. As putative Atl-host cell receptor, the heat shock cognate protein Hsc70 was identified by mass spectrometry. The importance of Hsc70 in internalization was demonstrated by the inhibition of S. aureus internalization with antiHsc70 antibodies. In conclusion, this novel Atl-or AtlE-mediated internalization mechanism may represent a 'back-up' mechanism in S. aureus internalization, while it may represent the major or even sole mechanism involved in the internalization of coagulase-negative staphylococci and thus may play an important role in the pathogenesis of chronic and relapsing infections with these serious pathogens.

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Section: Introductionmentioning

confidence: 99%

A novel staphylococcal internalization mechanism involves the major autolysin Atl and heat shock cognate protein Hsc70 as host cell receptor

Nina

Schlesier²,

Schmidt

et al. 2010

Cellular Microbiology

132

125

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“…There are 8 additional nonsynonymous polymorphisms in the fibronectin binding domain; however, none are as highly associated with human-pathogenic isolates. Adhesins such as Aas are important in the pathogenesis of S. saprophyticus urinary tract infections, and the gene encoding Aas has been previously implicated as a virulence factor ( 9 , 31 , 32 ).…”

Section: Resultsmentioning

confidence: 99%

“…UafA and Aas are found in all isolates, suggesting that they play important roles in the diverse niches occupied by S. saprophyticus . Aas has autolytic, fibronectin binding, and hemagluttinating functions ( 9 , 31 , 32 , 59 ). We identified a single, nonsynonymous polymorphism as a target of selection in the fibronectin binding repeats of Aas .…”

Section: Discussionmentioning

confidence: 99%

“…This implies that adoption of the pathogenic niche by S. saprophyticus has not entailed a tradeoff in its ability to live freely in the environment. A recent PCR-based survey of virulence factors in clinical and animal-associated isolates showed that dsdA , a gene encoding d -serine deaminase that is important for survival in urine ( 8 ), and uafA and aas , genes encoding adhesins that mediate binding to uroepithelium ( 9 , 10 ), were present in all isolates surveyed ( 11 ), suggesting an underlying pleiotropy, with these virulence factors playing important roles in the diverse environments occupied by S. saprophyticus .…”

Section: Introductionmentioning

confidence: 99%

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Adaptation in a Fibronectin Binding Autolysin of Staphylococcus saprophyticus

Mortimer

Annis

O’Neill

et al. 2017

mSphere

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Staphylococcus saprophyticus is an important cause of urinary tract infections (UTI) in women; such UTI are common, can be severe, and are associated with significant impacts to public health. In addition to being a cause of human UTI, S. saprophyticus can be found in the environment, in food, and associated with animals. After discovering that UTI strains of S. saprophyticus are for the most part closely related to each other, we sought to determine whether these strains are specially adapted to cause disease in humans. We found evidence suggesting that a mutation in the gene aas is advantageous in the context of human infection. We hypothesize that the mutation allows S. saprophyticus to survive better in the human urinary tract. These results show how bacteria found in the environment can evolve to cause disease.

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“…S. saprophyticus is primarily a pathogen of the urinary tract, and the factors involved in the mediation of UTI caused by S. saprophyticus are relatively well studied. S. saprophyticus possesses various types of adhesins such as hemagglutinins, autolysin Ass, and surface-associated lipases such as Ssp, which facilitate effective bacterial adherence to fibronectin and fibrinogen and, hence, colonization of the uroepithelium [12][13][14]. It can also form a bacterial biofilm, which confers an anti-phagocytic and antimicrobial-resistance phenotype and increases the virulence of the bacteria [15].…”

Section: Discussionmentioning

confidence: 99%

Emergence of Staphylococcus lugdunensis as a Cause of Urinary Tract Infection: Results of the Routine Use of MALDI-TOF MS

et al. 2020

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We analyzed the incidence and the clinical and laboratory characteristics of Staphylococcus lugdunensis urinary tract infections (UTIs) during a 10-year period (2009–2018) and compared them with those of Staphylococcus saprophyticus UTIs. A total of 38 and 162 episodes of S. lugdunensis and S. saprophyticus UTIs were observed. The number of S. saprophyticus UTIs was stable throughout the 10 years, whereas there was an obvious surge in the apparent number of S. lugdunensis UTIs since 2014, coinciding with the commencement of a routine use of MALDI-TOF MS. Univariate analysis showed that male sex (p < 0.001), advanced age (p < 0.001), hospital-acquired infections, (p < 0.001), upper UTI (p < 0.005), polymicrobial infections (p < 0.05), hypertension (p < 0.001), solid-organ malignancies (p < 0.001), renal stones (p < 0.001), urinary stricture (p < 0.05), vesicoureteral reflux (p < 0.001), and presence of a urinary catheter (p < 0.001) were significantly associated with S. lugdunensis UTI. Multivariable analysis revealed that S. lugdunensis UTI was associated with male sex (OR = 6.08, p < 0.05), solid-organ malignancies (OR = 12.27, p < 0.01), and urological system abnormalities (OR = 7.44, p < 0.05). There were significant differences in the patient population affected and predisposing factors between S. lugdunensis and S. saprophyticus UTIs.

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The hemagglutinin of Staphylococcus saprophyticus is a major adhesin for uroepithelial cells

Cited by 31 publications

References 16 publications

A novel staphylococcal internalization mechanism involves the major autolysin Atl and heat shock cognate protein Hsc70 as host cell receptor

A novel staphylococcal internalization mechanism involves the major autolysin Atl and heat shock cognate protein Hsc70 as host cell receptor

Adaptation in a Fibronectin Binding Autolysin of Staphylococcus saprophyticus

Emergence of Staphylococcus lugdunensis as a Cause of Urinary Tract Infection: Results of the Routine Use of MALDI-TOF MS

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