Estudo comparativo entre as técnicas de diluição em caldo e diluição em ágar, nos antibiogramas para Candida: a comparative study

Abstract

Os autores compararam o desempenho das técnicas de diluição em caldo e diluição em ágar, mediante a determinação da CIM (concentração inibitória mínima) e da CFM (concentração fungicida mínima) de antifúngicos poliênicos e imidazólicos, frente a diversas espécies de Candida. A concordância entre as técnicas foi variável em função do antifúngico utilizado. Os melhores percentuais de concordância ocorreram quando se realizou o teste com poliênicos. Os autores discutem aspectos que envolvem a problemática dos testes de sensibilidade de leveduras a antifúngicos

Cryptococcosis: a review of the brazilian experience for the disease

“…The E-test method was used by SILVA 88 for analysis of 62 isolated strains of pigeon feces, which were highly sensitive to AB, IZ and ketoconazole (KZ) and resistant to FZ for the most part. ALVES & CURY 3 observed less sensitivity to FZ in clinical samples than in environmental samples and to AB, IZ and KZ, the authors verifying high sensitivity of strains independent of their origin.…”

“…Occurrence of subclinical, asymptomatic forms of the disease is unknown and studies, such as cellular immunity surveys using intradermic tests with the yeast antigen in patients without clinical disease are rare, and therefore the extent of the subclinical form has not been properly evaluated 41,48 . C. neoformans' habitat in the environment is on the ground, in decomposed vegetables, bird and bat feces which are found both in urban and rural areas of Brazil 3,5,7,27,47,50,55,58,59,66,67,75,89,90 . Studies of LAZERA et al 43,44,45,46 gave new dimensions to the ecology of the cryptococcosis agent in Brazil demonstrating that yeast is associated not only to Eucalyptus sp gender, but also to other species of trees.…”

Growth Inhibition Effect of Brazilian Cerrado Plant Extracts on Candida Species

“…Candida strains were grown on Yeast Nitrogen Base Phosphate (YNBP) agar plates (Difco, USA). The Alves and Cury (1992) dilution method for MIC determination was employed. The inoculum suspensions were prepared from 6 h broth cultures diluted in 5 ml of sterile physiological Tris buffer (pH 7.4, 0.05 M) and adjusted to an OD (530 nm) of 0.05, equivalent to 1 ¥ 10 6 UFC/ml.…”