Enzyme-linked immunosorbent assay (ELISA) for measles antibody: a comparison with haemagglutination inhibition, immunofluorescence and plaque neutralization tests

Souza, Vanda Akico Ueda Fick de; Pannuti, Cláudio Sérgio; Sumita, Laura Masami; Albrecht, Paul

doi:10.1590/s0036-46651991000100007

Cited by 23 publications

(14 citation statements)

References 9 publications

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“…Measles IgG antibodies were detected by an in-house enzyme-linked immunosorbent assay as previously described (30). Antimeasles IgG antibody titers, expressed as milli-international units per milliliter, were estimated with serial dilutions of a reference serum provided by the National Institute for Biological Standards and Control (Hertford, England) that were included in each run of our enzyme-linked immunosorbent assay.…”

Section: Methodsmentioning

confidence: 99%

Identification of Primary and Secondary Measles Vaccine Failures by Measurement of Immunoglobulin G Avidity in Measles Cases during the 1997 São Paulo Epidemic

Pannuti

Morello

Moraes

et al. 2004

Clin Vaccine Immunol

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Despite almost universal use of measles vaccines in recent decades, epidemics of the disease continue to occur. Understanding the role of primary vaccine failure (failure to seroconvert after vaccination) and secondary vaccine failures (waning immunity after seroconversion) in measles epidemics is important for the evaluation of measles control programs in developing countries. After a measles epidemic in São Paulo, Brazil, 159 cases previously confirmed by detection of specific immunoglobulin M (IgM) antibodies were tested for IgG avidity, and a secondary immune response, defined by an IgG avidity index of at least 30%, was established in 30 of 159 (18.9%) patients. Among the 159 patients, 107 (67.3%) had not been vaccinated and 52 (32.7%) had received one or more doses of measles vaccine. Of the 107 unvaccinated patients, 104 (97.2%) showed a primary immune response, defined as an IgG avidity index of less than 30%. Among the 52 patients with documented vaccination, 25 (48.1%) showed a primary immune response and 27 (51.9%) showed a secondary immune response, thereby constituting a secondary vaccine failure. Primary vaccine failure was observed in 13 of 13 patients vaccinated prior to 1 year of age and in 43.5 and 12.5%, respectively, of patients receiving one or two doses after their first birthdays. These results provide evidence that measurement of IgG avidity can be used to distinguish between primary and secondary vaccine failures in vaccinated patients with measles; the method can also be a useful tool for the evaluation of measles control programs.

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Section: Methodsmentioning

confidence: 99%

Identification of Primary and Secondary Measles Vaccine Failures by Measurement of Immunoglobulin G Avidity in Measles Cases during the 1997 São Paulo Epidemic

Pannuti

Morello

Moraes

et al. 2004

Clin Vaccine Immunol

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show abstract

“…Interestingly, the questionnaires regarding the relationships between students revealed detailed behavioral patterns of students, and these actions were related to the division into groups 2 and 3 (data in preparation for publication). [14][15][16][17][18]. Many of these reports suggested the HI test was lower in sensitivity than EIA because falsenegative results were demonstrated in the HI test.…”

Section: Post-exposure Hi Titersmentioning

confidence: 99%

A case study of measles vaccination for university students during the measles outbreak in Tokyo, Japan, 2007

Fujisaki

Yamamura

Abe

et al. 2012

Journal of Infection and Chemotherapy

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“…Utilizou-se os dados antropomé-tricos da tabela de peso e altura de Santo André 74 . Os lactentes foram divididos em dois grupos: Grupo I (eutró-ficos) e Grupo II (desnutridos de 1°, 2° e 3° graus).…”

Section: Methodsunclassified

“…A reação de NP, considerada como "padrão ouro", não foi realizada. Não se pode, portanto, aferir a sensibilidade de cada método individualmente, como no estudo realizado por Souza et al (1991) 75 .…”

Section: Crianças Estudadasunclassified

Comparison of seroconversion rates between well-nourished and malnourished children after measles immunization at nine months of age

Mj¹,

Mm²,

Sp³

et al. 1996

J Pediatr (Rio J)

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311 Resumo AbstractThe study was based on activities developed at Embu, SP, between October 1989 and June 1990. Its purpose was to study serological turning after child vaccination against measles at the age of nine months. Two groups were compared, both within the same age limits. Group number I included eutrophic children and group number II included undernourished children. Gomes criteria was used to evaluate the children's nutritional state.Antibodies (AB) dosage was done through hemagglutination inhibition (HI) and ELISA. These two laboratory methods were also checked regarding its sensibility.Out of 130 children studied, 80 could be evaluated. From this total, 56 (70%) belonged to group I and 24 (30%) belonged to group II.When the ELISA method was used, a significantly higher seroconversion percentage (P < 0,05 or 5%) was found among children belonging to group II. This percentage was not detected when the HI method was used.J. pediatr. (Rio J.). 1996; 72(5):311-318: vaccine, measles, malnutrition, seroconversion. Viragem sorológica após a vacinação contra o sarampo, realizada em crianças eutróficas e desnutridas, aos nove meses de idade Comparison of seroconversion rates between well-nourished and malnourished children after measles immunization at nine months of age

show abstract

Enzyme-linked immunosorbent assay (ELISA) for measles antibody: a comparison with haemagglutination inhibition, immunofluorescence and plaque neutralization tests

Cited by 23 publications

References 9 publications

Identification of Primary and Secondary Measles Vaccine Failures by Measurement of Immunoglobulin G Avidity in Measles Cases during the 1997 São Paulo Epidemic

Identification of Primary and Secondary Measles Vaccine Failures by Measurement of Immunoglobulin G Avidity in Measles Cases during the 1997 São Paulo Epidemic

A case study of measles vaccination for university students during the measles outbreak in Tokyo, Japan, 2007

Comparison of seroconversion rates between well-nourished and malnourished children after measles immunization at nine months of age

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