Método fluorescente (diacetato de fluoresceína e brometo de etídeo) para o estudo da viabilidade de Cryptococcus neoformans em líquor

Corrêa, Benedito; Purchio, Adhemar; Paula, Claudete Rodrigues; Gambale, Walderez; Shikanai-Yasuda, Maria Aparecida

doi:10.1590/s0036-46651990000100008

Cited by 10 publications

(5 citation statements)

References 10 publications

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“…The live yeast form of fungus (LYH) was obtained and subcultured at 37°C on glucose‐cysteine‐blood BHI (Howard et al ., 1971). Yeast cells were used when the viability was over 95% by fluorescein diacetate and ethidium bromide staining (Corrêa et al ., 1990).…”

Section: Methodsmentioning

confidence: 99%

Leukotrienes are involved in leukocyte recruitment induced by live Histoplasma capsulatum or by the β‐glucan present in their cell wall

Medeiros

Silva

Malheiro

et al. 1999

British J Pharmacology

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1 The in¯ammatory cell in¯ux towards the peritoneal cavity in mice inoculated i.p. with live or dead Histoplasma capsulatum or with its subcellular preparations was studied. We also evaluated the e ects of dexamethasone (Dexa) or MK886, an inhibitor of leukotriene (LT) biosynthesis, on the recruitment of leukocytes. 2 Live yeast form of fungus (LYH) induced an increase in neutrophils (NE) which was highest 4 to 24 h after inoculation. Mononuclear cell (MN) migration beginning at 24 h with a gradual increase over 48 and 168 h, and an eosinophil (EO) recruitment occurs between 24 and 48 h. 3 NE and EO recruitment induced by dead mycelial form of fungus (DMH) was greater than that observed for dead yeast form of fungus (DYH). A similar leukocyte migration pattern was seen after i.p. injection of the alkali-insoluble fraction (F1) from DYH (F1Y) and F1 from DMH (F1M) this being more active than former. The di erence in concentration of b-glucan in DYH and DMH could explain the di erent in¯ammatory capacity exhibited by the two forms of H. capsulatum. 4 LT seems to be the principal mediator of leukocyte migration in response to LYH, DYH or DMH or to b-glucan. However, other mediators appear to contribute to NE and EO migration since the treatment with Dexa was more e ective in inhibiting cell migration than MK886.

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Section: Methodsmentioning

confidence: 99%

Leukotrienes are involved in leukocyte recruitment induced by live Histoplasma capsulatum or by the β‐glucan present in their cell wall

Medeiros

Silva

Malheiro

et al. 1999

British J Pharmacology

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“…According to Corrêa et al,(7), the intensive areas stained by FDA, demonstrate that SF1 are points which have high hydrolyzing activity due to acetillesterases. The Standard SF2 (weak fluorescence intensity) is explained by a weak action enzimatic, problaby allied to age of cells (6,12), but in this case, due to loss of enzyme activity by effetcs of radiation, because the cells had the same age (seven days).…”

Section: Samplesmentioning

confidence: 99%

Evaluation of viability of Aspergillus flavus and aflatoxins degradation in irradiated samples of maize

Aquino

Ferreira

Ribeiro

et al. 2005

Braz. J. Microbiol.

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One of the currently most important fungi in stored grains is Aspergillus flavus, which produce aflatoxins. This fungus can grow on diverse substrates and represents a serious public health and animal nutritional problem. Therefore, the study of techniques that can be applied to the control of aflatoxins is of great importance. The objective of the present study was to determine the effects of gamma radiation on the growth of Aspergillus flavus Link and on degradation of aflatoxin B1 and B2 (AFB1 and AFB2) at a relative humidity of 97 -99% and a water activity (Aw) of 0.88-0.94. Samples of corn grains were irradiated using a cobalt 60 source emitting gamma rays at doses of 2, 5 and 10 kGy. Irradiation was found to be effective in reducing the number colony-forming units of A. flavus, per gram, in the corn samples analyzed. In addition, the fluorescent viability test (fluorescein diacetate and ethidium bromide) revealed a decrease in the number of viable cells with increasing irradiation doses and three different fluorescence patterns. Furthermore, irradiation induced a partial reduction in AFB 1 and AFB 2 levels at the doses of 2 and 5 kGy, whereas complete degradation of aflatoxins was observed in the assay employing 10 kGy.

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“…Em [17] foi feita a análise de viabilidade de células em microscópio de fluorescência. No entanto, o método, apesar de sensível e simples, é oneroso e perigoso para o operador, pois os corantes utilizados são potencialmente cancerígenos.…”

Section: Trabalhos Relacionadosunclassified

“…Os autores de [17] propuseram a validação do programa BioVic, com o objetivo de comparar os resultados obtidos na contagem automática de leveduras viáveis e inviáveis baseado em visão computacional, com os métodos de contagem por plaqueamento e visão humana. As amostras foram coradas com azul de metileno e as leveduras viáveis e inviáveis foram contadas em câmara de Neubauer.…”

Section: Trabalhos Relacionadosunclassified

Automatic Yeast Detection and Counting Using Computer Vision Techniques

Gomide

Cunha

Gomide³

2021

Anais Do XVII Workshop De Visão Computacional (WVC 2021)

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This paper presents the development of a computer vision system that automatically identifies and counts viable and inviable brewer's yeast, to improve the time and accuracy of results obtained compared to the manual expert counting method commonly performed in the brewing industry. The equipment used consists of a digital video camera coupled to an optical microscope, which transmits the captured images, in real time, to the computer. Two approaches were tested and implemented, one taking into account the morphology and color of yeasts, and the other using machine learning. Although there are programs that automatically count yeasts, this is the first application that makes use of convolutional neural network techniques with Yolo to identify yeasts, making the results more accurate and reliable compared to manual methods. Experiments were carried out to measure the performance and accuracy of the prototype, which are presented in this article.

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Método fluorescente (diacetato de fluoresceína e brometo de etídeo) para o estudo da viabilidade de Cryptococcus neoformans em líquor

Cited by 10 publications

References 10 publications

Leukotrienes are involved in leukocyte recruitment induced by live Histoplasma capsulatum or by the β‐glucan present in their cell wall

Leukotrienes are involved in leukocyte recruitment induced by live Histoplasma capsulatum or by the β‐glucan present in their cell wall

Evaluation of viability of Aspergillus flavus and aflatoxins degradation in irradiated samples of maize

Automatic Yeast Detection and Counting Using Computer Vision Techniques

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