Analysis of the specificity of human antibodies to antigens of Leishmania braziliensis braziliensis

Abstract

The antigenicity of promastigotes of Leishmania braziliensis braziliensis (L. b.braziliensis) treated with 1% sodium desoxycholate in 10 mM Tris-Hcl pH 8.2 was analysed by immunoblot using as probes sera from American cutaneous leishmaniasis (ACL), American visceral leishmaniasis (AVL), schistosomiasis, malaria and Chagas' disease. The ACL sera reacted constantly with a 60 kD band. No reactivity to this protein was observed with sera from the other diseases above mentioned indicating that the 60 kD protein may be used in serodiagnosis for ACL.

Evaluation of antigens from various Leishmania species in a Western blot for diagnosis of American tegumentary leishmaniasis.

“…[46][47][48] The immunoblot results obtained with soluble protein extracts of Leishmania species and strain 268T showed a complex reactivity pattern (Figures 1-4), in which most (93%) of the polypeptide fractions of parasites were recognized by at least one serum sample from the individuals tests. Similarly complex profiles were found 15,49 when the human antibody specificity to antigens of L. (V.) braziliensis and L. infantum was analyzed by Western blotting.…”

“…20 Relatively high molecular weights were found for the most frequently reacting protein fractions of L. braziliensis panamensis (78-120 kD) 18 and L. (V.) braziliensis (69-250 kD). 20 When we compared individual reactive protein fractions, a 60-kD protein from L. (V.) braziliensis had been previously identified by human sera from patients with ATL, 15 whereas we detected a 63-kD antigen (Table 4). In the L. (L.) amazonensis extract, a 74-kD fraction was recognized by 75.47% of the ATL-positive sera, whereas in L. tropica and strain 268T, the recognition was much lower (Table 4).…”

“…This method has used soluble extracts, 15,18,20,21,44 purified membrane proteins, 45 or recombinant proteins as antigens. [46][47][48] The immunoblot results obtained with soluble protein extracts of Leishmania species and strain 268T showed a complex reactivity pattern (Figures 1-4), in which most (93%) of the polypeptide fractions of parasites were recognized by at least one serum sample from the individuals tests.…”

“…1,13 The humoral immune response occurs only during the active phase of infection, with the appearance of low titers of antibodies, representing a temporary response. 14,15 Several techniques have been developed for the serologic diagnosis of leishmaniasis. These include an immunofluorescence assay (IFA), an enzyme-linked immunosorbent assay (ELISA), and the Western blot.…”

Diagnosis of American cutaneous leishmaniasis by enzyme immunoassay in patients from Northern Paraná State, Brazil

“…Among the serological tests used for the diagnosis are the direct agglutination, indirect immunofluorescence (IIF), and enzyme immunoassay (EIA). Some EIA techniques for the ACL diagnosis were described by several authors that used L. braziliensis [11][12][13][14] and other Leishmania species [15][16][17] as antigen, and they detected IgG [12,14,15,17,18], IgM [12] or IgA antibodies [13].…”