Isolamento, identificação e sorotipagem de candida albicans a partir de secreção vaginal

Garcia, Antonio Luengo; Siqueira, Antonio Martins de

doi:10.1590/s0036-46651988000400004

Cited by 3 publications

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References 13 publications

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“…Candida species can be found in the gastrointestinal tract of 20 to 80% of healthy adults, becoming pathogenic when host immune conditions are altered by offending factors (1). Of the invasive infections caused by Candida spp., blood infections, also known as candidemias, are the most clinically relevant (2).…”

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confidence: 99%

Candidemia in a brazilian tertiary hospital: microbiological and clinical features over a six-year period

Mondelli¹,

Niéro-Melo²,

Bagagli³

et al. 2012

J. Venom. Anim. Toxins incl. Trop. Dis

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Abstract:Yeasts are becoming a common cause of nosocomial fungal infections in immunocompromised patients. Such infections often develop into sepsis with high mortality rates. The aim of this study was to evaluate some of the numerous factors associated with the development of candidemia. Medical records were retrospectively analyzed of 98 Candida spp. patients. Results showed that the most prevalent risk factors for developing candidemia were: antibiotics and antifungal agents (93.9% and 79.6%, respectively); the use of central venous catheter (93.9%); mechanical ventilation (73.5%); and parenteral nutrition (60.2%). The main species of Candida found were: C. parapsilosis (37.76%), C. albicans (33.67%); and others (28.57%). C. glabrata showed the highest mortality rate (75%), followed by C. tropicalis (57.1%) and C. albicans (54.5%). The elevated mortality rate found in this study indicates that preventive measures against candidemia must be emphasized in hospitals.Key words: candidemia, mortality, associated factors, Candida albicans. Original PaPer The Journal of Venomous Animals and Toxins including Tropical Diseases ISSN 1678-9199 | 2012 | volume 18 | issue 2 | pages 244-252 INTRODUCTIONSeveral authors have reported a global increase in yeast infections over the past 20 years. Candida species can be found in the gastrointestinal tract of 20 to 80% of healthy adults, becoming pathogenic when host immune conditions are altered by offending factors (1). Of the invasive infections caused by Candida spp., blood infections, also known as candidemias, are the most clinically relevant (2). Candida spp. is currently one of the five main causes of blood infections in hospitals, and an increase in candidemia cases has been reported mainly in patients using antibiotics, under immunosuppressive therapy or parenteral nutrition, and in patients exposed to multiple invasive procedures (3,4). Candidemia is one of the most common blood infections in tertiary hospitals; it has also been associated with long stay in hospitals and high mortality (5-8).Although Candida albicans is the most frequent species isolated in patients with fungemia, cases of infections caused by other Candida species have increased (9-15). The frequency of C. albicans and other Candida species found in patients depends, among other factors, on the analyzed population and used therapy (16)(17)(18)(19). The most commonly found species in Brazilian candidemia patients are C. albicans, Candida parapsilosis, and Candida tropicalis (20). MATERIALS AND METHODSWe analyzed 98 yeast samples from different patients admitted to Botucatu Medical School University Hospital (HC-FMB) between 2000 and 2006 along with their medical records (obtained from the Statistical Medical Service -SAME, owned by HC-FMB). These patients developed symptoms of candidemia and samples were Sample size was calculated using the Fisher and Belle formula, with a 95% confidence interval and 5% precision for expected prevalence of fungemia patients. Beck-Sagué and Jarvis (21) study was used a...

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Section: Introductionmentioning

confidence: 99%

Candidemia in a brazilian tertiary hospital: microbiological and clinical features over a six-year period

Mondelli¹,

Niéro-Melo²,

Bagagli³

et al. 2012

J. Venom. Anim. Toxins incl. Trop. Dis

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Vaginal yeast flora of pregnant women in the Gusco region of Peru

Vidotto

Ochoa

Ponce

et al. 1992

Mycoses

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Summary. A study of the vaginal yeast flora in pregnant women living in Cusco and in its region (Peru), located approximately 3000 m above sea level, is reported. We observed 300 pregnant, healthy and non‐diabetic women who attended a gynaecological clinic in the Lorena, Regional or IPSS (Institute Peruano de Seguridad Social) hospitals in Cusco. A comprehensive clinical history was obtained from each patient. It included age, work, parity, time of pregnancy, use of contraceptives or antibiotics, type of vaginal symptoms, type and amount of vaginal secretion. The yeasts were isolated from 44.3% of the cases. The positive cases were more frequently found in the following categories: 20–30 year‐old patients (69.2%), ninth month of pregnancy (49.6%), first pregnancy (41.4%), no delivery (58.7%) and no abortion (66.9%). Most women complained of leukorrhea (75.9%) with mucus, scarce without odour (18.8%) or lumpy, regular, without odour (15.8%) secretion. The yeasts isolated were Candida albicans (66.2%), other Candida species (12.8%), Torulopsis glabrata (8.3%) or other Torulopsis species (2.2%), Saccharomyces cerevisiae (7.5%), Rhodotorula sp. (1.5%) and Tricho‐sporon cutaneum (1.5%). Zusammenfassung. Es wird über die vaginale Hefeflora bei Schwangeren in Cusco, Peru, und Umgebung berichtet, einer Region, die etwa 3000 m über dem Meeresspiegel liegt. In die Studie eingeschlossen wurden 300 schwangere, nicht‐diabetische, anderweitig gesunde Frauen, die die gynäkologische Kliniken in Cusco oder in der Lorena‐Region aufsuchten. Von jeder Patien‐tin wurde ein ausführlicher klinischer Bericht aufgenommen, der Alter, Beschäftigung, Zahl der Schwangerschaften, Schwangerschaftsstatus, frü‐here Anwendung von Kontrazeptiva und Anti‐biotika, Art der vaginalen Symptomatik sowie Typ und Ausmaß der Vaginalsekretion umfaßte. Von 44.3% der Patientinnen wurden Hefen iso‐liert. Hefepositivität wurde häufiger in folgenden Kategorien gefunden: 20‐30 Jahre alte Patientinnen (69.2%), neunter Schwangerschaftsmonat (49.6%), Erstschwangerschaft (41.4%), Frauen ohne bisherige Entbindung (58.7%), Frauen ohne bisherigen Abort (66.9%). Die meisten Frauen klagten über Leukorrhoe (75.9%) mit Schleimfluß, wenig Ausfluß jedoch mit Geruch (18.8%) oder klumpige regelmäßige Sekretion ohne Geruch (15.8%). Die isolierten Hefearten waren Candida albicans (66.2%), andere Candida‐Arten (12.8%), Torulopsis glabrata (8.3%), andere Torulopsis‐Arten (2.3%), Saccharomyces cerevisiae (7.5%), Rhodotorula‐Arten (1.5%) und Trichospo‐ron cutaneum (1.5%).

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CARACTERÍSTICAS FENOTIPICAS DE CEPAS DE Cândida ISOLADAS DE PACIENTES COM CANDIDÍASE VAGINAL

Ribeiro¹,

Silva²,

Fernandes³

et al. 2007

Rev Patol Trop

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As 63 cepas de Cândida, previamente isoladas de 120 mulheres com suspeita clínica de candidíase vaginal atendidas na cidade de Goiània-GO e identificadas segundo Kregcn-Van RJJ, foram caracterizadas fenotipicamente quanto aos aspectos morfológicos, biológicos e soroiógicos. Nos 57 isolados de C. albicans verificou-se com maior incidência o morfotípo 5330 dentre os sete morfotipos presenciados. Nas 4 cepas de C. krusei foram detectadas os morfotipos 0000, 5230 e 5330 e, nos dois cultivos de C. tropicalis, os morfotipos, 5220 e 5240. A biotipagem de Odds & Abbott mostrou os biótipos 377 e 777 nos isolados de C albicans, Nas cepas de C. kritseí e de C, tropicalis detectou-se o biótipo 677. Quanto à sorotipagem dos isolados de C. albicans houve o predomínio do soroíipo A em 5ó (98,2%) das cepas. UNITERMOS: Fenotipagem, Candidíase vaginal. Cândida spp. INTRODUÇÃOEm busca de melhor caracterização e individualização dos isolados de Cândida, várias metodologias de tipagem fenotípica têm sido desenvolvidas, diante da necessidade de informações de caráter epidemiológico, de auxílio na identificação precisa do patógeno e suas relações filogenéticas (20,22). A caracterização fenotípica das cepas de Cândida compreende o estudo de aspectos morfológicos, biológicos e sorológicos das leveduras.O modelo de morfotipagem para os isolados de Cândida é baseado nas diferenças morfológicas entre as colónias de espécie de Cândida

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Isolamento, identificação e sorotipagem de candida albicans a partir de secreção vaginal

Abstract: RESUMO(11,9% ) isoladas a partir de mulheres da raça branca e 03 (18,75%r) da raça negra.

Cited by 3 publications

References 13 publications

Candidemia in a brazilian tertiary hospital: microbiological and clinical features over a six-year period

Candidemia in a brazilian tertiary hospital: microbiological and clinical features over a six-year period

Vaginal yeast flora of pregnant women in the Gusco region of Peru

CARACTERÍSTICAS FENOTIPICAS DE CEPAS DE Cândida ISOLADAS DE PACIENTES COM CANDIDÍASE VAGINAL

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