An ELISA method using serum derived HDAg for the sorological detection of HDV antigens and antibodies

Abstract

SUMMAßYOne of the main difficulties related to the detection of'the lfepatitis Delta virus (HDV) antigen

New Enzyme-Linked Immunosorbent Assay for Detection of Antibodies against Hepatitis Delta Virus Using a Hepatitis Delta Antigen Derived from a Taiwanese Clone and Comparison to the Abbott Radioimmunoassay

“…To date, various serological enzyme-linked immunosorbent assays (ELISAs) have been developed for the diagnosis of HDV infection, in which HD antigen (HDAg) mainly came from liver tissues of HDV-infected animals or serum of HDV-infected individuals, and this can pose barriers to effective quality control (6,9). In the present study, we have established an ELISA for the detection of anti-HD in human serum utilizing a specific recombinant HDAg (rHDAg) protein cloned from the local dominant HDV strain, and we have evaluated this new assay by comparing it with a commercially available radioimmunoassay (RIA).…”

“…For anti-HD antibody detection, various RIAs and enzyme immunoassays (EIAs) have been developed (4,8,9,17,18). One major drawback of these tests is that they use HDAg derived from either infected liver or infectious serum of humans or animals (e.g., chimpanzees and woodchucks) (15).…”