Anti-leishmania igA immunoenzymatic assay in mucocutaneous leishmaniasis (Preliminary report)

Abstract

The Authors describe an anti-Leishmania IgA-ELISA assay in mucocutaneous leishmaniasis. Increased titers were found in leishmaniasis patients, mainly in the first and second year of infection and in deep mycoses patients showing either mucosal involvement or widespread disease.

Immunoglobulin A antibodies to Trypanosoma cruzi antigens in digestive forms of Chagas' disease

“…The presence of IgA antibodies in the acute phase of Chagas' disease, as observed here, was an expected finding, since these class-specific antibodies have pathogenic signif-icance and diagnostic value in several nonrelated acute infections (12,19,20). The results shown in Table 2 suggest that IgA antibodies in acute Chagas' disease differ in specificity from IgA antibodies related to the digestive forms.…”

“…In patients with digestive tract involvement, the finding of IgA antibodies in serum seems to be significant and might constitute an early signal of active mucosal lesions of, for example, mucocutaneous leishmaniasis (12, 21) and mycoses with mucous membrane or respiratory tract implications (3). However, specific IgA antibodies in serum are not observed in advanced cases of mucocutaneous leishmaniasis with a long history of destructive lesions (12). The persistence of IgA antibodies in the digestive forms of chronic Chagas' disease is thus an unexpected finding.…”

Detection of specific antibody isotypes and subtypes before and after treatment of American visceral leishmaniasis

“…The prognostic value of total or specific IgA in active AVL patients has to be further investigated because it seems to correlate with severe clinical forms in some diseases such as mucocutaneous leishmaniasis (27,28), the digestive form of chronic Chagas' disease (29), and HIV infection in drug users (30).…”

Diagnosis of American cutaneous leishmaniasis by enzyme immunoassay in patients from Northern Paraná State, Brazil

“…Serological cross-reactivity between leishmaniasis and Chagas' disease has been described by several authors [9,16]. Reports of cross-reactivity among cutaneous leishmaniasis and paracoccidioidomycosis for IgA antibodies were referred by Guimarães et al [13]. The knowledge of this fact is very relevant, mainly in areas where the two In spite of the detected cross-reactivity, when IgG-EIA was used for diagnosis in patients with cutaneous ulcers suspicious of ACL, the test detected 97.0% of positive parasitological search, and it was negative in 97.0% of the patients that had both parasitological search and the Montenegro's skin test negatives.…”

“…Among the serological tests used for the diagnosis are the direct agglutination, indirect immunofluorescence (IIF), and enzyme immunoassay (EIA). Some EIA techniques for the ACL diagnosis were described by several authors that used L. braziliensis [11][12][13][14] and other Leishmania species [15][16][17] as antigen, and they detected IgG [12,14,15,17,18], IgM [12] or IgA antibodies [13].…”