Avaliação de antígenos de Cysticercus cellulosae no imunodiagnóstico da cisticercose humana pela hemaglutinação indireta

Nascimento, Evaldo; Mayrink, Wilson

doi:10.1590/s0036-46651984000600001

Cited by 14 publications

(9 citation statements)

References 3 publications

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“…Few reports have simultaneously compared the usefulness of different antigen preparations from T. solium cysticerci for the serodiagnosis of neurocysticercosis. In these studies, performed mainly with IH and ELISA tests, there is a marked variation in the efficacies of cysticercal antigens, with sensitivities ranging from 47% to 95% and specificities ranging from 67% to 100% (Nascimento & Mayrink 1984; Costa 1986; Larralde et al. 1986; Nascimento et al.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Taenia solium and Taenia crassiceps cysticercal antigens for the serodiagnosis of neurocysticercosis

Arruda

Silva

Quagliato

et al. 2005

Tropical Med Int Health

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SummaryWe evaluated the usefulness of seven cysticercal antigen extracts, four from Taenia solium cysticerci (whole parasite-TsoW, membrane-TsoMe, vesicular fluid-TsoVF and scolex-TsoSc) and three from T. crassiceps cysticerci (whole parasite-TcraW, membrane-TcraMe and vesicular fluid-TcraVF), for serodiagnosis of neurocysticercosis with an enzyme-linked immunosorbent assay (ELISA). Cysticercusspecific IgG were screened in serum samples from 23 patients with neurocysticercosis, 32 patients with other infections and 48 healthy persons. The best results were obtained with the TsoVF-ELISA (91.3% sensitivity; 96.2% specificity) and TcraVF-ELISA (91.3% sensitivity; 95% specificity). The ELISA done with whole parasite and membrane extracts from cysts of T. solium and T. crassiceps and the scolex extract from T. solium cysts showed a low performance in terms of sensitivity, ranging from 47.8% to 73.9%. None of the antigen preparations from T. solium and T. crassiceps cysticerci used in this study showed outstanding performance for the serodiagnosis of neurocysticercosis. However, considering the results obtained with the seven antigen preparations, vesicular fluid from T. solium and T. crassiceps cysticerci may be useful for detecting specific antibodies in sera from patients with neurocysticercosis.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Taenia solium and Taenia crassiceps cysticercal antigens for the serodiagnosis of neurocysticercosis

Arruda

Silva

Quagliato

et al. 2005

Tropical Med Int Health

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“…[68] Pathak and Gaur (1985)[69] reported the cyst fluid to contain a pH of 6.9 – 7, ash (4.89%), dry matter (55.46%), organic substance (49.57%), and crude protein (1.89%). They reported the scolex to contain 8.20% ash, 65.26% dry matter, 57.20% organic substance, and 8.35% crude protein, while the membrane contained 6.69% ash, 63.36% dry matter, 53.30% organic substance, and 5.25% crude protein.…”

Section: Antigens Of the Cysticercimentioning

confidence: 99%

Cysticercus cellulosae antigens in the serodiagnosis of neurocysticercosis

Parija

Gireesh

2011

Trop Parasitol

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Neurocysticercosis (NCC) is difficult to diagnose clinically because of its varied clinical presentation. However, an accurate diagnosis is possible only after suspicion on epidemiological grounds, proper interpretation of the clinical data, analysis of the findings on imaging studies, and specific immunological tests on the serum and cerebrospinal fluid (CSF). The diagnosis of NCC by any single parameter thus continues to remain difficult. In the past, detection of NCC was based on autopsy studies and histological confirmation. In recent times, the advent of imaging methods such as computed tomography and magnetic resonance imaging have provided excellent non-invasive tools for easy detection of NCC. Nevertheless, an imaging technique of the brain, although useful, is not considered as a gold standard for the diagnosis of NCC. Serological tests are being increasingly used in adjunct with imaging techniques, to aid the diagnosis of NCC. Immunodiagnostic techniques include detection methods for specific antibodies and for circulating parasite antigens in the serum and CSF. Currently, many of the immunodiagnostic tests, including the enzyme-linked immunosorbent assay and enzyme immunotransfer blot, use purified native antigens for the immunodiagnosis of NCC. Nevertheless, the main problem with the use of native cysticercal antigens is that the native proteins often show cross reactions with sera from humans infected with other parasites. The preparation of native antigens also demand a constant supply of parasitic material from the intermediate host pig. In order to overcome the problems in using native antigens, the recombinant antigens or synthetic peptides, which can be produced under stable conditions, are being evaluated for the serodiagnosis of NCC.

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“…On the other hand, crude extract of total cysticerci, soluble extracts from parts of cysticerci (membrane or scolex), crude extract of Taenia solium, or semipuri®ed proteins have been used as antigens by many researchers (Biagi et al 1961;Powell et al 1966;Proctor et al 1966;Flisser et al 1980;Nascimento and Mayrink 1984;Nascimento et al 1987a;Tsang et al 1989).…”

Section: Discussionmentioning

confidence: 99%

“…In this work, parasite-speci®c antibodies were detected in 92% of sera from patients with neurocysticercosis by IH, in 50% by CFT, and in 3% by ID. We have previously reported that an antigen prepared from scolices of cysticerci of T. solium increases sensitivity and speci®city when used in the IH test (Nascimento and Mayrink 1984).…”

Section: Introductionmentioning

confidence: 99%

Immunological evaluation of a 26-kDa antigen from Taenia solium larvae for specific immunodiagnosis of human neurocysticercosis

Maia

Pianetti

et al. 1999

Parasitology Research

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Human neurocysticercosis, due to infection of the central nervous system by cysticerci of Taenia solium, is a severe form of neurologic disease occurring in Central and South America. Specific proteins from scolex antigen from cysticerci were purified by polyacrylamide gel electrophoresis and electroelution and recognized in Western blots by antibodies present in sera from patients with neurocysticercosis. The proteins appeared as 13-, 17-, and 26-kDa bands on Coomassie blue-stained gels and proved to be specific to cysticerci of T. solium. No cross-reactivity with sera from patients with taeniasis or hydatidosis was observed. Enzyme-linked immunosorbent assay using the purified proteins of 13, 17, and 26 kDa demonstrated rates of 53%, 88%, and 100% specificity, respectively, at the cutoff serum dilution of 1:32 for the specific immunodiagnosis of human neurocysticercosis.

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Avaliação de antígenos de Cysticercus cellulosae no imunodiagnóstico da cisticercose humana pela hemaglutinação indireta

Cited by 14 publications

References 3 publications

Evaluation of Taenia solium and Taenia crassiceps cysticercal antigens for the serodiagnosis of neurocysticercosis

Evaluation of Taenia solium and Taenia crassiceps cysticercal antigens for the serodiagnosis of neurocysticercosis

Cysticercus cellulosae antigens in the serodiagnosis of neurocysticercosis

Immunological evaluation of a 26-kDa antigen from Taenia solium larvae for specific immunodiagnosis of human neurocysticercosis

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