Antigenemia in paracoccidioidomycosis: probable demonstration of circulating antigen by counterimmunoelectrophoresis test: preliminary report

Rodrigues, Maria Conceição; Cassaguerra, Cintia Marzagão; Lacaz, Carlos da Silva

doi:10.1590/s0036-46651984000500011

Cited by 22 publications

(13 citation statements)

References 13 publications

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“…In the past some investigators have tried to detect circulating antigen in PCM patients using polyvalent antigens or antibodies in different assays such as competition enzyme-linked immunosorbent assay (ELISA) [35] immunoradiometric assay [36], CIE [37], immunoelectrophoresis-immunodiffusion [38], passive hemagglutination inhibition [39], inverted linear immunoelectrophoresis [40], and immunoblotting [41]; but all of these assays presented low sensitivities. Gómez et al [42] were the first to use monoclonal antibodies (Mabs) to detect the 87-kDa circulating antigen in PCM patients by inhibition ELISA (inh-ELISA), which showed 80.4% sensitivity.…”

Section: Antigen Detectionmentioning

confidence: 99%

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Serology of paracoccidioidomycosis

Camargo

2008

Mycopathologia

113

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This review provides the background for understanding the role of a battery of diagnostic methods in paracoccidioidomycosis (PCM). This systemic mycosis is a disease endemic in many regions of Latin America, with sporadic cases also occurring throughout the world (mycosis of importation). Although excellent laboratory methods for diagnosis are available, there are deficiencies that must be met by continued research. Understanding the uses and limitations of a battery of laboratory methods is essential to diagnose PCM. Clinicians and laboratory directors must be familiar with the uses and limitations of a battery of serologic and mycological tests to accurately diagnose of PCM. Antibody and antigen detections are valuable adjuncts to histopathology and culture. More recently, the gp43 and gp70 antigen detection assay have improved the methodology of diagnosis of this mycosis, which improves reproducibility and facilitates monitoring antigen clearance during antifungal treatment. Furthermore, detection of antigen in cerebrospinal fluid and in bronchoalveolar lavage fluid increases the sensitivity for diagnosis of PCM in central nervous system and in pulmonary infections, respectively.

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Section: Antigen Detectionmentioning

confidence: 99%

“…Some tests have been used to detect gp43 circulating antigen with partial success in PCM [24,35,[37][38][39][40][41][42].…”

Section: Antigenemia During Therapymentioning

confidence: 99%

Serology of paracoccidioidomycosis

Camargo

2008

Mycopathologia

113

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“…Antigen detection tests may be more effective than antibody tests for diagnosing paracoccidioidomycosis, particularly in immunocompromised individuals and in those who have previously been exposed to P. brasiliensis (80). However, the detection of antigenemia in paracoccidioidomycosis patients also has some difficulties because (i) insensitive reagents and methodology were used and (ii) cross-reactions occurred in patients with histoplasmosis, aspergillosis, and cryptococcosis (66,70,181).…”

Section: Paracoccidioidomycosismentioning

confidence: 99%

Current Status of Nonculture Methods for Diagnosis of Invasive Fungal Infections

2002

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The incidence of invasive fungal infections has increased dramatically in recent decades, especially among immunocompromised patients. However, the diagnosis of these infections in a timely fashion is often very difficult. Conventional microbiologic and histopathologic approaches generally are neither sensitive nor specific, and they often do not detect invasive fungal infection until late in the course of disease. Since early diagnosis may guide appropriate treatment and prevent mortality, there has been considerable interest in developing nonculture approaches to diagnosing fungal infections. These approaches include detection of specific host immune responses to fungal antigens, detection of specific macromolecular antigens using immunologic reagents, amplification and detection of specific fungal nucleic acid sequences, and detection and quantitation of specific fungal metabolite products. This work reviews the current status and recent developments as well as problems in the design of nonculture diagnostic methods for invasive fungal infections

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“…Some researchers have previously tried to detect circulating antigen in PCM patients using polyvalent an-tigens or antibodies in different assays such as enzyme-linked immunosorbent assay (ELISA) competition (14), immunoradiometric assay (10), immunoelectrophoresis-immunodiffusion (15), counterimmunoelectrophoresis (32), passive hemagglutination inhibition (20), inverted linear immunoelectrophoresis (19), and immunoblotting (26). Goméz et al (16) were the first investigators to use monoclonal antibodies for the detection of an 87-kDa circulating antigen in PCM with 80.4% sensitivity.…”

mentioning

confidence: 99%

Detection of Circulating gp43 Antigen in Serum, Cerebrospinal Fluid, and Bronchoalveolar Lavage Fluid of Patients with Paracoccidioidomycosis

Silva¹,

Colombo

Blotta

et al. 2003

J Clin Microbiol

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Paracoccidioidomycosis (PCM) is an important systemic fungal disease, particularly among individuals living and working in rural areas of endemicity in Latin America, who, without antifungal therapy, may develop fatal acute or chronic infection. For such patients, the detection of antibody responses by immunodiffusion is of limited value due to false-negative results. In contrast, the detection of Paracoccidioides brasiliensis gp43 circulating antigen may represent a more practical approach to the rapid diagnosis of the disease. Accordingly, an inhibition enzyme-linked immunosorbent assay (inh-ELISA) was developed for the detection of a 43-kDa P. brasiliensis-specific epitope incorporating a species-specific murine monoclonal antibody. With sera from patients with acute and chronic forms of the disease (n ‫؍‬ 81), the overall sensitivity of the test was found to be 95.1%, while specificity was found to be 97.5% compared to that with normal human sera from blood donors

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Antigenemia in paracoccidioidomycosis: probable demonstration of circulating antigen by counterimmunoelectrophoresis test: preliminary report

Cited by 22 publications

References 13 publications

Serology of paracoccidioidomycosis

Serology of paracoccidioidomycosis

Current Status of Nonculture Methods for Diagnosis of Invasive Fungal Infections

Detection of Circulating gp43 Antigen in Serum, Cerebrospinal Fluid, and Bronchoalveolar Lavage Fluid of Patients with Paracoccidioidomycosis

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