Depressão humoral específica em pacientes crônicos infectados pelo Trypanosoma cruzi

Brenière, Simone Frédérique; Poch, Olivier; Selaes, Hugo; Tibayrenc, Michel; Lemesre, Jean-Loup; Antezana, Gerardo; Desjeux, Philippe

doi:10.1590/s0036-46651984000500005

Cited by 19 publications

(1 citation statement)

References 7 publications

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“…This phenomenon could be more common than expected as revealed by previous reports in endemic nations such as Argentina, Bolivia, Venezuela, and Brazil (12,13,33). Some of its possible causes include: a) window-phase or very low antibody titers in the early stage of infection (34); b) inability of some T. cruzi strains to induce specific antibody production (35); c) HIV co-infection causing decreased antigen presentation due to virus tropism on CD4 + T-, dendritic, and natural killer cells, and the consequent impairment of their antibody secretion (36); d) non-responder phenomenon or persistent antibody-negative (immunosilent) carriers who are unable to produce a detectable humoral response and present appropriate antigens (possibly associated with CD4 + and CD8 + T-cell depletion due to the affinity of the thymus as a T. cruzi-infection target), as well as the reduction of IL-2 (a pivotal cytokine in cellular memory immunity and antigen recognition) (34,35), and e) procedural testing errors.…”

Section: Discussionmentioning

confidence: 99%

Disagreement between PCR and serological diagnosis of Trypanosoma cruzi infection in blood donors from a Colombian endemic region

et al. 2021

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Introduction: Chagas’ disease is the leading cause of infectious myocarditis worldwide. This infection caused by Trypanosoma cruzi is usually life-long and asymptomatic; however, the third part of infected people can develop severe or even fatal cardiomyopathy. As the parasitemia in the chronic phase is both low-grade and intermittent, T. cruzi infection is principally detected by serology, although this method has sensitivity and specificity limitations.Objective: To determine the level of agreement between serologic and molecular tests in 658 voluntary blood donors from six provinces in the Colombian department of Santander.Materials and methods: We evaluated an array of diagnostic technologies by cross-section sampling performing a serological double diagnostic test for T. cruzi antibody detection (Chagas III ELISA™, BiosChile Group, and ARCHITECT Chagas CMIA™, Abbott), and DNA detection by polymerase chain reaction (PCR). We collected the demographic, clinical, and epidemiological information of participants. The sample size was calculated using Epidat™ and the statistical analysis was done with Stata 12.1™.Results: PCR was six times more sensitive in detecting T. cruzi infection than ELISA/CMIA with prevalence values of 1.8% (12/658) and 0.3% (2/658), respectively, and kappa=0.28 (95%CI: -0.03 - 0.59). In contrast, serology showed a sensitivity of 16.7% (95%CI: 2.09 - 48.4) and a specificity of 100% (95%CI: 99.4 - 100). All seropositive samples were found to be positive by PCR.Conclusions: The implementation of PCR as a complementary method for screening donors could reduce the probability of false negative and the consequent risk of transfusional-transmission of Chagas’ disease, especially in endemic regions.

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Section: Discussionmentioning

confidence: 99%

Disagreement between PCR and serological diagnosis of Trypanosoma cruzi infection in blood donors from a Colombian endemic region

et al. 2021

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Modelling the Transmission of Trypanosoma cruzi: The Need for an Integrated Genetic Epidemiological and Population Genomics Approach

Tibayrenc

2010

Advances in Experimental Medicine and Biology

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Blood culture and polymerase chain reaction for the diagnosis of the chronic phase of human infection with Trypanosoma cruzi

Castro

Luquetti

Rassi

et al. 2002

Parasitology Research

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In the present study, we evaluated for the first time the profile of blood parasitism in untreated, chronic Chagas' disease. The study was conducted on 60 patients and a control group of nine serologically negative individuals. Analysis of three blood samples showed 70% cumulative positivity for blood culture and 86.7% positivity for PCR. The comparison of the two tests revealed that 41.1% (74/180) of the samples presented positive results for both PCR and blood culture, 22.2% (40/180) were positive for PCR alone, and 4.4% (8/180) were positive for blood culture and negative for PCR. The addition of the second sample raised positivity significantly for both blood culture ( P=0.0000) and PCR ( P=0.0369). Addition of the third sample was also statistically significant for blood culture ( P=0.0001) but not for PCR ( P=0.1186). These data point to the importance of studying the parasitemia of Trypanosoma cruzi-infected individuals before specific treatment. They also suggest that at least two blood samples should be collected and that two tests should be used, if possible--a procedure that considerably improves the parasitologic diagnosis of Chagas' disease and the evaluation of therapeutic efficacy.

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Depressão humoral específica em pacientes crônicos infectados pelo Trypanosoma cruzi

Abstract: SUMMARYWe performed a comparatÍve study bebween

Cited by 19 publications

References 7 publications

Disagreement between PCR and serological diagnosis of Trypanosoma cruzi infection in blood donors from a Colombian endemic region

Disagreement between PCR and serological diagnosis of Trypanosoma cruzi infection in blood donors from a Colombian endemic region

Modelling the Transmission of Trypanosoma cruzi: The Need for an Integrated Genetic Epidemiological and Population Genomics Approach

Blood culture and polymerase chain reaction for the diagnosis of the chronic phase of human infection with Trypanosoma cruzi

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