Detecção de DNA de Leishmania braziliensis em pacientes de leishmaniose tegumentar americana

Martins, Leila; Alexandrino, Aline C.; Guimarães, Georgia

doi:10.1590/s0034-89102010005000004

Cited by 14 publications

(12 citation statements)

References 5 publications

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“…Of the asymptomatic infected dogs, 72% were identified by PCR, demonstrating the effectiveness of this technique for the diagnosis of infection in these animals. Martins et al (2010) also demonstrated the efficiency of PCR carried out using peripheral venous blood samples for detection of L. braziliensis DNA in humans with no cutaneous lesions. According to Rodrigues (2000), this method displays significantly higher sensitivity and specificity than direct testing techniques, isolation from culture, and IFA.…”

Section: Discussionmentioning

confidence: 89%

“…Antunes Uchôa et al (2001) also state that the humoral immune response in CL cases is weak, and in most cases mediated by T lymphocytes. In addition, Martins et al (2010) found that PCR tests can be positive even years after lesions have healed. They found that of the 93 patients in their study without lesions, 37 (39.8%) were PCR positive, indicating the presence of parasite-derived genetic material.…”

Section: Discussionmentioning

confidence: 99%

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Diagnosis and epidemiology of canine leishmaniasis in southeastern Bahia, Brazil

et al. 2016

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ABSTRACT. Leishmaniasis is a disease caused by protozoa of the genus Leishmania. Two distinct forms are recognized: visceral leishmaniasis (VL) and cutaneous leishmaniasis (CL). In the Americas, the causative agent of VL is L. infantum chagasi, whereas L. braziliensis is principally responsible for CL. Domestic dogs constitute the main source of VL in urban environments, and have also been implicated in CL epidemiology. We carried out molecular and serological surveys to detect Leishmania infection in dogs from the municipality of Ituberá in Bahia, Brazil. Furthermore, we identified risk factors associated with illness in dogs from this locality. Blood samples were collected from 399 dogs and tested using an indirect immunofluorescence assay (IFA) and polymerase chain reaction (PCR) to detect Leishmania spp antibodies and L. infantum chagasi and L. braziliensis DNA, respectively. Dogs were clinically evaluated and tissue samples from those exhibiting skin lesions were examined for parasites. In addition, the dog owners completed an epidemiological questionnaire to identify factors associated with infection. Skin lesions consistent with CL were found on 37 (9.3%) of the evaluated animals, but parasitological examination was negative for all samples. The IFA returned positive results for 60 (15%) dogs. PCR identified DNA from L. braziliensis in 86 (21.6%) animals, where as all samples proved negative for L. infantum chagasi. The 134 dogs (33.6%) testing positive using IFA and/ or PCR were considered infected, and of these, only 13 demonstrated skin lesions. Animals from rural areas were 3.39-times more likely to be infected compared to those in urban environments.

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Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Diagnosis and epidemiology of canine leishmaniasis in southeastern Bahia, Brazil

et al. 2016

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“…In spite of a robust immune response, a small number of parasites persist following the resolution of leishmaniasis (Mendonca et al, 2004; Figueroa et al, 2009; Martins et al, 2010). In mice infected with L. braziliensis in the ear dermis, parasites also persist in draining lymph nodes, despite lesion resolution and parasite clearance from the infection site (de Moura et al, 2005).…”

Section: Immunosuppression In Leishmaniasismentioning

confidence: 99%

The immunobiology of Leishmania braziliensis infection

Oliveira¹,

Brodskyn²

2012

Front. Immun.

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Leishmaniases are a group of diseases caused by protozoa of the genus Leishmania that affect millions of people worldwide. These diseases are caused by distinct Leishmania species, of which L. braziliensis, a New World representative of the Leishmania genus, has been the least studied. Although leishmaniasis caused by L. braziliensis induces a range of clinical manifestations ranging from mild localized lesions to severe mucosal involvement, few studies have focused on elucidating the immune mechanisms behind this pathology. In this review, we focus on the immunobiology of L. braziliensis infection, emphasizing the innate and adaptive immune responses and taking into consideration both studies performed in endemic areas and experimental models of infection. Additionally, we address recent findings regarding the role of sand fly saliva in disease immunopathogenesis and vaccine development.

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“…Invasive sampling methods are difficult to perform in settings with limited resources and without specialized training 22,23 . Additionally, biopsies can often be delicate, uncomfortable and painful for the patient 24 , and may cause bleeding at the biopsy site. The majority of researches on the detection of Leishmania DNA focus on biopsy samples, and few studies have evaluated the use of swabs, a relatively noninvasive sampling method.…”

Section: Introductionmentioning

confidence: 99%

Efficiency of noninvasive sampling methods (swab) together with Polymerase Chain Reaction (PCR) for diagnosing American Tegumentary Leishmaniasis

Boni

Oyafuso

Soler

et al. 2017

Rev. Inst. Med. trop. S. Paulo

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Traditional diagnostic methods used to detect American Tegumentary Leishmaniasis, such as histopathology using biopsy samples, culture techniques, and direct search for parasites, have low sensitivity and require invasive collection procedures. This study evaluates the efficiency of noninvasive sampling methods (swab) along with Polymerase Chain Reaction (PCR) for diagnosing American Tegumentary Leishmaniasis using skin and mucous samples from 25 patients who had tested positive for leishmaniasis. The outcome of the tests performance on swab samples was compatible with PCR results on biopsy samples. The findings have also shown that PCR-kDNA test is more efficient than PCR-HSP70 and qPCR tests (sensitivity of 92.3%, 40.7%, and 41%, respectively). Given the high sensitivity of the tests and the fact that the sampling method using swabs affords greater patient comfort and safety, it could be said that this method is a promising alternative to conventional biopsy-based methods for the molecular diagnosis of leishmaniasis.

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Detecção de DNA de Leishmania braziliensis em pacientes de leishmaniose tegumentar americana

Cited by 14 publications

References 5 publications

Diagnosis and epidemiology of canine leishmaniasis in southeastern Bahia, Brazil

Diagnosis and epidemiology of canine leishmaniasis in southeastern Bahia, Brazil

The immunobiology of Leishmania braziliensis infection

Efficiency of noninvasive sampling methods (swab) together with Polymerase Chain Reaction (PCR) for diagnosing American Tegumentary Leishmaniasis

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