Controle de vetores utilizando mosquitos geneticamente modificados

Wilke, André Barretto Bruno; Gomes, Almério de Castro; Natal, Délsio; Marrelli, Mauro Toledo

doi:10.1590/s0034-89102009005000050

Cited by 30 publications

(21 citation statements)

References 27 publications

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“…[36] This reduced competitiveness of the male mosquitoes can be overcome by transgenic techniques in which a dominant conditional lethal factor can be expressed in the male mosquitoes. [37] In this Release of Insects carrying a Dominant Lethal (RIDL)[38] technique, the released mosquitoes on mating transfer the lethal gene to the progeny and its expression will lead to embryonic lethality. This technique is employed in producing A. aegypti mosquitoes which can pass the lethal factor to the offsprings, causing embryonic lethality.…”

Section: Eradication Of Malariamentioning

confidence: 99%

Newer approaches to malaria control

Damodaran

Pradhan

2011

Trop Parasitol

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Malaria is the third leading cause of death due to infectious diseases affecting around 243 million people, causing 863,000 deaths each year, and is a major public health problem. Most of the malarial deaths occur in children below 5 years and is a major contributor of under-five mortality. As a result of environmental and climatic changes, there is a change in vector population and distribution, leading to resurgence of malaria at numerous foci. Resistance to antimalarials is a major challenge to malaria control and there are new drug developments, new approaches to treatment strategies, combination therapy to overcome resistance and progress in vaccine development. Now, artemisinin-based combination therapy is the first-line therapy as the malarial parasite has developed resistance to other antimalarials. Reports of artemisinin resistance are appearing and identification of new drug targets gains utmost importance. As there is a shift from malaria control to malaria eradication, more research is focused on malaria vaccine development. A malaria vaccine, RTS,S, is in phase III of development and may become the first successful one. Due to resistance to insecticides and lack of environmental sanitation, the conventional methods of vector control are turning out to be futile. To overcome this, novel strategies like sterile insect technique and transgenic mosquitoes are pursued for effective vector control. As a result of the global organizations stepping up their efforts with continued research, eradication of malaria can turn out to be a reality.

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Section: Eradication Of Malariamentioning

confidence: 99%

Newer approaches to malaria control

Damodaran

Pradhan

2011

Trop Parasitol

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“…However, such compounds are responsible for environmental damage and the development of resistant mosquito populations (Bellinato et al 2016, Hemingway et al 2004. Furthermore, these compounds present high residual power, remaining in environments for long periods of time, and wide action spectrum, reaching other organisms, including vertebrate, in aquatic environments (Wilke et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Effects of diflubenzuron on associated insect fauna with Anopheles(Diptera: Culicidae) in laboratory, partial-field, and field conditions in the Central Amazon

Ferreira

Arcos

Maia

et al. 2020

An. Acad. Bras. Ciênc.

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This study aimed to analyze the possible effects of diflubenzuron on the associated insect fauna under laboratory, semi-field and field conditions. Laboratory bioassays were performed in aquariums with mortality readings (%) every 24h until 96h, and in semi-fields, insects were kept in basins with readings every 24h for up to 12 days, in triplicates and a control. In the field experiment, a collection was performed before the application of diflubenzuron in ten brick factory pits (25m²) and 15 post-application. The values of LC 50 and LC 90 for Chironomus were 2.77x10-3g/L and 0.86g/L, respectively, and for Buenoa, they were 0.019g/L and 0.92g/L, a strong relationship was observed between mortality and exposure time (r²>0.8) in all concentrations used. In semi-field, similar mean values of emergency inhibition were observed, except for Euthyplociidae, which was more sensitive. There was no significant difference between the data of richness and diversity of aquatic insects in the field experiment, considering an interval of 15 days (p=0.32). Finally, the data suggest that diflubenzuron may have a negative effect on the associated insect fauna, but in the field experiment the environmental conditions of Anopheles breeding sites may have affected its efficiency.

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“…Também não insere novos genes na população alvo, tendo em vista que os indivíduos liberados são estéreis (HENDRICHS et al, 2002). A técnica SIT possui uma vantagem em relação ao uso de agentes químicos e remoção de criadouros, pois utiliza o fato de que machos são competentes na busca por fêmeas da mesma espécie, aumentando a efetividade da técnica conforme a população é reduzida (ALPHEY, 2002;ALPHEY et al, 2008;WILKE et al, 2009).…”

Section: Alternativas De Controle Aos Inseticidas Eunclassified

“…Dessa forma, para que ocorra uma diminuição drástica da população de insetos vetores de patógenos de doenças humanas, animais e de pragas agrícolas deve haver um estudo prévio da população de insetos nas áreas onde os machos estéreis serão introduzidos. Os machos estéreis devem ser capazes de concorrer com os machos férteis e copularem com as fêmeas selvagens (WILKE et al, 2009).…”

Section: Alternativas De Controle Aos Inseticidas Eunclassified

“…A necessidade de liberação exclusiva de mosquitos machos é segura à saúde pública e deve-se ao fato destes não exercerem a hematofagia, pois são as fêmeas adultas que se alimentam de sangue e transmitem os agentes patogênicos (WILKE et al, 2009). Por este motivo, a liberação de uma população de machos estéreis na natureza deve ser cuidadosamente inspecionada para que não haja contaminação desta população com fêmeas estéreis, pois estas podem comprometer o sucesso do programa ao inibir o acasalamento dos machos estéreis com as fêmeas selvagens, com risco de efeito neutralizador (MCINNIS et al, 1994).…”

Section: Alternativas De Controle Aos Inseticidas Eunclassified

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Padronização de técnica para produção em massa de 'Culex quinquefasciatus' (Diptera: Culicidae)

Marchi¹

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Marchi, M. J. Standardization of technique for mass production of Culex quinquefasciatus (Diptera: Culicidae) [Master Dissertation]. São Paulo (BR): Faculdade de Saúde Pública da USP; 2014. Objective-To establish a method for mass rearing of Culex (Culex) quinquefasciatus mosquitoes in laboratory, in order to provide subsidy to the population control of this species through the release of sterile males. Methods-A colony of Cx. quinquefasciatus was founded from immature collected in January 2013 in the Pinheiros River, São Paulo/SP, Brazil. Adult mosquitoes had their dry weights measured and the development was evaluated as the time to reach pupae and adult phase. In the first experiment it was tested four different diets (fish food, dog food, a mix of 50% fish and 50% dog food or a mix of 33.33% fish 33.33% dog food and 33.33% milk powder) and three temperatures (27 ± 2°C, 23 ± 2°C and 20 ± 2°C). In the second experiment it was tested several conditions of food amounts (mg/day), larvae densities (ml of water/larva) and water column height that would generate high development rate, low immature mortality and high adult dry weight. Results-In the first experiment there was no statistically significant difference in the time for the development to pupae stage or in the total dry weight of adults among the four experimental diets. However, fish food provided less immature mortality. The highest temperature (27 ± 2°C) decreased the time of the immature life cycle, but generated adults with lower dry weights. In the second experiment, the condition 0.5 mg/larva, 5 ml/larva in 2.1 cm of water column and 27 ± 2°C offered good mass rearing and generated higher development rate, lower immature mortality and higher adult dry weights. Conclusion-No ideal diet was defined for mass rearing in the parameters analyzed. The less expensive diet was the dog food, but this should be milled by hand or machine, which makes the fish food more practical in the production trays. This study suggested that the best condition for mass rearing of Cx. quinquefasciatus mosquitoes was 0.5 mg of daily fish food/larva, 5 ml/larva in 2.1 cm of water column and 27 ± 2°C. The study suggests that this technique can assist in population control of this species.

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Controle de vetores utilizando mosquitos geneticamente modificados

Cited by 30 publications

References 27 publications

Newer approaches to malaria control

Newer approaches to malaria control

Effects of diflubenzuron on associated insect fauna with Anopheles(Diptera: Culicidae) in laboratory, partial-field, and field conditions in the Central Amazon

Padronização de técnica para produção em massa de 'Culex quinquefasciatus' (Diptera: Culicidae)

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