Surveys and surveillances were carried out in the ber growing regions of Punjab through fixed plot surveys in Fruit Research Farm, Punjab Agricultural University (PAU), Ludhiana, Punjab during 2018 and 2019 to record the emerging insect pests and their natural enemies. During these surveys, infection of a fungus was observed on the adults of blow fly, Chrysomya megacephala (Fabricius) (Diptera: Calliphoridae) occurring on ber plants in PAU, Ludhiana. This fly is economically significant as pollinator of fruit crops. The adult flies were observed to be dead and were covered with fungal mycelial growth. Later on, the fungus was identified as an entomopathogenic fungus, Cladosporium cladosporioides based on morphological and molecular characterization. This is the first record of infection of an entomopathogenic fungus, C. cladosporioides on this pollinator dipteran fly, C. megacephala from Punjab, India. Thus, the potential of C. cladosporioides for the control of other dipteran insect-pests of fruit crops can be explored.