Detection of anti-Giardia lamblia serum antibody among children of day care centers

Guimarães, Semíramis; Sogayar, Maria Ignês L

doi:10.1590/s0034-89102002000100010

Cited by 9 publications

(6 citation statements)

References 16 publications

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“…However, antibodies to cysticercosis decreased rapidly when stored on filter paper 81. Detection of exposure to giardiasis suffered from low specificity, possibly reflecting cross-reactivity or long-term persistence of antibodies 83,84…”

Section: Resultsmentioning

confidence: 99%

“…Using commercially available assays, sensitivities of > 90% may be achieved 62,66. Leishmaniasis, cysticercosis, and giardiasis have proved to be less promising in the few studies that have evaluated DBS compared with a recognized gold standard 75,81,83. Serological tests for leptospirosis, treponemal infections, and some rickettsia have yielded excellent results,90,92,94 whereas others, such as brucellosis, have been less successful 89…”

Section: Discussionmentioning

confidence: 99%

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An Overview of the Clinical Use of Filter Paper in the Diagnosis of Tropical Diseases

Smit¹,

Elliott²,

Peeling³

et al. 2014

The American Journal of Tropical Medicine and Hygiene

114

108

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Tropical infectious diseases diagnosis and surveillance are often hampered by difficulties of sample collection and transportation. Filter paper potentially provides a useful medium to help overcome such problems. We reviewed the literature on the use of filter paper, focusing on the evaluation of nucleic acid and serological assays for diagnosis of infectious diseases using dried blood spots (DBS) compared with recognized gold standards. We reviewed 296 eligible studies and included 101 studies evaluating DBS and 192 studies on other aspects of filter paper use. We also discuss the use of filter paper with other body fluids and for tropical veterinary medicine. In general, DBS perform with sensitivities and specificities similar or only slightly inferior to gold standard sample types. However, important problems were revealed with the uncritical use of DBS, inappropriate statistical analysis, and lack of standardized methodology. DBS have great potential to empower healthcare workers by making laboratory-based diagnostic tests more readily accessible, but additional and more rigorous research is needed.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

An Overview of the Clinical Use of Filter Paper in the Diagnosis of Tropical Diseases

Smit¹,

Elliott²,

Peeling³

et al. 2014

The American Journal of Tropical Medicine and Hygiene

114

108

View full text Add to dashboard Cite

show abstract

“…Giardia intestinalis (Giardia lamblia), hem gelişmiş hem de gelişmekte olan ülkelerde en sık görülen insan bağırsak paraziti olup, dünya çapında dağılım göstermektedir. Endemik bölgelerde anaokulu ve ilkokul çağındaki çocuklarda Giardia intestinalis (G. intestinalis) prevalansının çok yüksek olduğu belirtilmiştir (1)(2)(3). Giardiazis, Türkiye'nin Güneydoğu Anadolu Bölgesi'nde de çocuklarda en sık görülen bağırsak protozoon hastalığıdır (4,5).…”

Section: Introductionunclassified

Comparison of Direct Microcopy and Antigen Casette Tests for the Detection of Giardia and Cryptosporidium

Doni

Zeyrek²,

Gürses³

et al. 2013

TurkiyeParazitolDerg

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When we compared the results of the direct microscopy and antigen casette tests, we found no significant difference between them for test reliability (p > 0.05). Antigen tests have higher sensitivity (100%) and specifity (100%) than the modified acid-fast staining technique, therefore, it is a preferred reference method . However, an experienced staff working accurately might access the same conclusion. Considering the cost of antigen tests, direct microscopic examination is cheaper, andeasier when it used by an experienced person.

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“…High rates of anti- Giardia IgG have been reported in Mexico, where anti- Giardia IgG was found in 77% of the sera of lactating women [ 33 ] and in 55.3% of the general population [ 12 ]. Guimarães and Sogayar [ 34 ] found an anti- Giardia IgG seroprevalence of 63.3% in children at daycare centers of São Paulo, Brazil. Other studies have shown a reduction in the prevalence of G. duodenalis and other enteroparasitoses infections in developing countries due to improvements in hygienic-sanitary and educational conditions in these regions [ 27 , 35 ], thus reducing exposure to these pathogens.…”

Section: Discussionmentioning

confidence: 99%

Specific IgG and IgA Antibody Reactivities in Sera of Children by Enzyme-Linked Immunoassay and Comparison With Giardia duodenalis Diagnosis in Feces

et al. 2020

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Background: Giardia duodenalis is conventionally diagnosed in fecal samples using parasitological methods. However, sensitivity is poor when only a single sample is analyzed, due to intermittent excretion of cysts in feces. Alternatively, the serum antibodies to G. duodenalis can be used for parasite diagnosis and epidemiological studies to determine previous exposure. We compared the rate of G. duodenalis infection between serum anti-Giardia IgG and IgA antibodies and fecal examination in Brazilian children. Methods: Fecal and serum samples were tested from 287 children at a clinical laboratory and from 187 children at daycare centers. Fecal samples were processed using conventional parasitological methods and coproantigen detection for Giardia diagnosis. Serum samples were tested using an in-house ELISA for detection of anti-Giardia IgG and IgA. Results: G. duodenalis was found in 8.2% (N = 39) of the 474 children analyzed. The sensitivity and specificity of ELISA were 80.0% and 90.0% for IgG and 80.0% and 83.3% for IgA, respectively. The total positivity rate of anti-Giardia IgG and IgA in the sera was 13.9% (N = 66) and 23.6% (N = 112). The agreement between the positivity of specific antibodies and the detection of G. duodenalis in feces was moderate for ELISA-IgG, kappa index (95% CI) = 0.543 (0.422-0.664), and mild for ELISA-IgA, kappa index (95% CI) = 0.283 (0.162-0.404). Among the children infected with other enteroparasites, 11.6% (N = 10) and 24.4% (N = 21) showed reactivity to anti-Giardia IgG and to IgA, respectively. This cross-reactivity was more frequent in samples from children infected with Endolimax nana and Entamoeba coli. Conclusions: The higher frequency of specific antibody reactivity compared with G. duodenalis diagnosis in feces could reflect continuous exposure of children to G. duodenalis infection, resulting in long-lasting immunological memory and/or cross-reactivity with other intestinal amoebas.

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Detection of anti-Giardia lamblia serum antibody among children of day care centers

Cited by 9 publications

References 16 publications

An Overview of the Clinical Use of Filter Paper in the Diagnosis of Tropical Diseases

An Overview of the Clinical Use of Filter Paper in the Diagnosis of Tropical Diseases

Comparison of Direct Microcopy and Antigen Casette Tests for the Detection of Giardia and Cryptosporidium

Specific IgG and IgA Antibody Reactivities in Sera of Children by Enzyme-Linked Immunoassay and Comparison With Giardia duodenalis Diagnosis in Feces

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