Diafanização de esfregaços de fezes

Ferreira, Cláudio Santos; Carvalho, Maria Esther de

doi:10.1590/s0034-89101972000100003

Cited by 3 publications

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“…Another way to tackle this problem is to reduce light-scattering by means of refractive index matching. In that case the optical properties of fecal suspensions are changed without the removal of any fraction of it 1 . Cedar wood oil, well known for its high refractive index (n = 1.52), has excellent optical characteristics as a clearing medium, but requires previous dehydration of the fecal smears.…”

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Refractive index matching applied to fecal smear clearing

Ferreira

2005

Rev. Inst. Med. trop. S. Paulo

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Thick smears of human feces can be made adequate for identification of helminth eggs by means of refractive index matching. Although this effect can be obtained by simply spreading a fleck of feces on a microscope slide, a glycerol solution has been routinely used to this end. Aiming at practicability, a new quantitative technique has been developed. To enhance both sharpness and contrast of the images, a sucrose solution (refractive index = 1.49) is used, which reduces the effect of light-scattering particulates. To each slide a template-measured (38.5 mm3) fecal sample is transferred. Thus, egg counts and sensitivity evaluations are easily made.

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