Composite synthetic hydroxyapatite 30%, in two physical states, as dermal filler

Abstract

RESUMO Compósito de hidroxiapatita sintética 30%, em dois estados físicos, como preenchedor de relevo cutâneoObjetivou-se avaliar a resposta tecidual, após implantação de hidroxiapatita sintética 30% (HAP-91 ® ), em dois estados físicos, para preenchimento cutâneo. Foram utilizados 18 coelhos da raça Nova Zelândia, distribuídos aleatoriamente em dois grupos ® fluida e viscosa) e, posteriormente, divididos em três subgrupos, de acordo com o período pós-implante, em oito, 21 e 49 dias. Em cada grupo foi implantado 1,0 mL de HAP-91 ® , fluida ou viscosa, no tecido subcutâneo, 1 cm cranial à crista da escápula direita. Foi realizada a mensuração da espessura cutânea, imediatamente antes e durante 15 dias após a implantação do compósito. Avaliações de sensibilidade dolorosa foram realizadas, atribuindo-se os escores: 0 -quando o animal permitiu o toque e não manifestou reação; 1 -quando permitiu o toque, mas manifestou reação de dor, como aumento da frequência respiratória ou tentativa de fuga; 2 -quando não permitiu o toque. Aos oito, 21 e 49 dias, foi realizada a biópsia da região implantada. Não foi observada diferença entre a espessura cutânea dos animais (p > 0,05) e todos os animais receberam escore 0 para sensibilidade dolorosa. Na análise histológica, não foi notado processo inflamatório evidente. Houve predomínio de células mononucleares, nas amostras de oito dias, e uma organização tecidual ao redor do biomaterial, com tendência ao encapsulamento. A HAP-91 ® , tanto fluida quanto viscosa, é biocompatível e indicada para o preenchimento cutâneo.Palavras-chave: implante cutâneo, compósito, medicina estética. The aim of this study was to evaluate the response to the implantation of synthetic hydroxyapatite 30% (HAP-91 ® ) in different physical states as dermal filler. Eighteen New Zealand rabbits were used, distributed randomly into two equal groups and then divided into three groups according to the postoperative period at 8, 21 and 49 days. One mL of HAP-91 ® , fluid and viscous, was implanted in the subcutaneous tissue, 1 cm proximal to the cranial crest of the right scapula. The thickness of the skin was measured before and after implantation and for the following 15 days. Pain sensitivity assessment was conducted, assigning the following scores: 0 -when the animal allowed the touch of the implant area and expressed no signs of pain; 1 -when the animal allowed the touch, but pain reaction occurred, like increase of the respiratory rate or attempt to escape; 2 -when the animal did not allow the touch to the implanted area. At 8, 21 and 49 days, biopsy of the implanted area was performed. No difference was observed between the thickness of the skin (p>0.05) and all animals received a score 0 for soreness. Histological analysis did not reveal any obvious inflammatory process, showing a predominance of mononuclear cells in samples of eight days and tissue organization around the biomaterial with a tendency to encapsulation. The results indicate that HAP-91 ® , both viscous and fluid, is biocompatible...

In vitro characterization of hydroxyapatite and cobalt ferrite nanoparticles compounds and their biocompatibility in vivo

“…This tissue had characteristics of an active tissue, rich in fibroblasts and blood vessels (hyperemia in varying degrees). These findings are characteristic of the normal reaction of the organism when in contact with an implant, prosthesis, or biomaterial [53], and were also observed by Carlo et al [36], who observed fibroblast proliferation and formation of fibrous tissue around HAp particles implanted in subcutaneous tissue of dogs and by Sepúlveda et al [50], when implanting HAp in subcutaneous tissue of rabbits. In the HCoγ 1 sample, this fibrous tissue appeared to be more organized, tending to encapsulate the material.…”

“…The postoperative observations indicated a good acceptance of the compounds, since macroscopically no inflammatory reaction was observed and no rejection of the implanted compounds occurred as well. Carlo et al [ 36 ], Yanagida et al [ 49 ], and Sepúlveda et al [ 50 ] obtained similar results in their studies with Hap associated with bioglass, HAp covering lactic acid, and synthetic HAp for skin filling, respectively.…”

“…The tissue organization around a biomaterial implanted in soft tissue, with a progressive tendency to encapsulation, was observed by Li et al [ 66 ], Sepúlveda et al [ 50 ], and Carlo et al [ 36 ]. According to these authors, a complex surface, which is the case of the composites used in this study, may be favorable in bone to promote cell adhesion; however, in soft tissues, the same surface may cause mechanical discomfort to the tissues and, consequently, encapsulation as a defense mechanism.…”

“…Carlo et al [ 36 ] and Sepúlveda et al [ 50 ] observed a large amount of multinucleated giant cells around the HAp particles implanted in the subcutaneous tissue of dogs. In the present work, they were visualized only in HCoγ1 and HC samples, but in small amounts and only at 15 days postoperatively.…”

Effect of the Structural and Morphological Properties of Surfactant-Assisted Hydroxyapatite on Dermal Irritation and Antibacterial Activity

“…Sepulveda et al [ 48 ] evaluated synthetic hydroxyapatite HAP-91 ® as a dermal filler; they showed that this intradermal implant tends to encapsulate itself and does not cause signs of toxicity but only presents an initial inflammatory response.…”