Chemical sterilization in in vitro propagation of Arundina bambusifolia Lindl. and Epidendrum ibaguense Kunth

Rodrigues, Donizetti Tomaz; Novais, Roberto Ferreira; Venegas, Víctor Hugo Álvarez; Dias, José Maria Moreira; Otoni, Wagner Campos

doi:10.1590/s0034-737x2013000400001

Cited by 11 publications

(7 citation statements)

References 12 publications

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“…However, prolonged sterilization leads to increase in mortality rate. The negative effect of longer exposure was also reported by many scientists [27,28,29]. Obtaining of uncontaminated explants does not guarantee its establishment.…”

Section: Resultsmentioning

confidence: 83%

Effect of Surface Sterilants on in vitro Establishment of Pineapple (Ananas comosus (L.) Merill.) cv. Kew

Parveen

Mir

Ranjan

et al. 2019

CJAST

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The rate of pineapple propagation through conventional technique is quite low and time taken and that by seed is apparently hard to germinate. Non-availability of quality planting material is one of the major constraints for expansion of its cultivation area in Bihar. Keeping this in view an experiment was conducted for in vitro establishment for large scale disease free planting materials production. The most commonly encountered problem during in vitro pineapple germplasm establishment is the rate of contamination, which is very high in case of pineapple. Suckers of pineapple cultivar Kew were used as explants for the study. In this experiment the efficiency of three sterilizing agents (Clorox, HgCl2 and NaOCl) at different concentrations and duration was evaluated in terms of number of aseptic cultures. Results revealed that when no sterilant was used all the cultures were contaminated. The contamination of explants significantly decreased with increase in concentration of different sterilants and their time of exposure. The highest survival of explants (58.31±1.71%) were observed when explants were treated with Clorox 40% for 20 minutes which also resulted in 17.89±0.25% and 25.03±2.63% mortality and per cent contamination respectively. As the duration of Clorox 40% was increased, percent contamination decreased but simultaneously increased the mortality rate at 25 mins of duration. The percent survival also increased when explants were treated with different concentrations of HgCl2 and NaOCl at different time durations. However, NaOCl treatments were less effective as compared to other two sterilants.

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Section: Resultsmentioning

confidence: 83%

Effect of Surface Sterilants on in vitro Establishment of Pineapple (Ananas comosus (L.) Merill.) cv. Kew

Parveen

Mir

Ranjan

et al. 2019

CJAST

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“…The treatment with 4.2 g L -1 of NaClO (143 mL L -1 of CB) for 5 min was, for most species, more effective in controlling contamination, with 87% of germination of the seeds. Rodrigues et al (2013) observed a negative effect on germination with increasing concentration of NaClO, which ranged from 50 to 250 ml L -1 of CB. Such effect was assigned to the disinfectant toxicity.…”

Section: Introductionmentioning

confidence: 91%

“…For the140 mL plastic flasks no significant difference was observed in the proportion of oxidized seeds between the control treatment and the lowest concentration of CB; those with 340 mL, the oxidation was higher ISSN 2166-0379 2017 with the use of CB (Figure 1a). This variation on the seed oxidation is due to, in the case of glass flasks, probably the activated charcoal, which adsorbs toxic compounds, such as phenols, produced by plants, reducing the seeds and the protocorms oxidation (Thomas 2008;Rodrigues et al 2013). In the control treatment, the culture medium was autoclaved inside the flasks and, after its cooling, there was a small decantation of the activated charcoal in the bottom of the flask.…”

Section: Journal Of Agricultural Studiesmentioning

confidence: 99%

Chemical Disinfestation for Sowing and in Vitro Re-cultivation of Orchids and its Influence on the Seedlings Nutrition

Fontes¹,

Álvarez²,

Novais³

et al. 2017

JAS

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The use of a commercial bleach solution (CB) for in vitro propagation of orchids has proven to be an efficient technique and an alternative to the use of the autoclave and laminar flow chamber (LFC). The aim of this study was to evaluate the use of CB in disinfestation of culture media (CM) for seeding and for re-cultivation of orchid plantlets and its effect in different types of flasks and on the nutritional state of the plantlets. In the seeding experiment, seeds of Laelia tenebrosa Rolfe were used, with evaluation of three types of flasks; five concentrations of CB applied to the CM of 2, 5, 10, 20 and 40 mL L -1 and the control treatment. In the re-cultivation experiment, Cattleya kerri Brieger & Bicalho plantlets were used. The rates of CB added to the CM were 2, 5, 10, 20, 30, 35 and 38 mL L -1 in polypropylene containers, and the rates sprayed on the plantlets at re-cultivation were 5, 12.5, 25, 50, 75, 87.5 and 95 mL L -1 . The proportion of germinated seeds and the dry matter weight of the protocorms were greater in the control treatment. The increasing rates of CB added to the CM had worse results for germination in glass flasks. In re-cultivation, there was no significant difference in the growth of the plantlets between the autoclaved flasks and those disinfested in CB.Keymessage: Both orchid seeding and re-cultivation of seedlings can be carried out entirely and efficiently without the use of an autoclave and laminar flow chamber, using chemical disinfection with commercial bleach.

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“…Il a été incorporé au milieu de culture chez le manioc (Hounguè et al, 2017). L'hypochlorite de sodium, largement utilisé en phase de désinfection de surface du matériel végétal (Lopez-Aranda et al, 1994 ;Zuraida et al, 2011 ;Dossoukpèvi et al, 2012) a également été introduit dans le milieu de culture chez certaines espèces (Pierik, 1987 ;Rodrigues et al, 2013). L'ananas, du fait de sa forte teneur en sucre, renferme plusieurs germes rendant difficiles sa désinfection en culture in vitro.…”

Section: Introductionunclassified

Contrôle des germes endogènes par usage d’agents antimicrobiens et réponse de différents explants durant la phase d’initiation <i>in vitro</i> de l'ananas (<i>Ananas comosus</i> (L.) Mill var. comosus)

Badou¹,

Pita²,

Houédjissin³

et al. 2018

J. App. Bioscience.

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Chemical sterilization in in vitro propagation of Arundina bambusifolia Lindl. and Epidendrum ibaguense Kunth

Cited by 11 publications

References 12 publications

Effect of Surface Sterilants on in vitro Establishment of Pineapple (Ananas comosus (L.) Merill.) cv. Kew

Effect of Surface Sterilants on in vitro Establishment of Pineapple (Ananas comosus (L.) Merill.) cv. Kew

Chemical Disinfestation for Sowing and in Vitro Re-cultivation of Orchids and its Influence on the Seedlings Nutrition

Contrôle des germes endogènes par usage d’agents antimicrobiens et réponse de différents explants durant la phase d’initiation <i>in vitro</i> de l'ananas (<i>Ananas comosus</i> (L.) Mill var. comosus)

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