Estudo comparativo entre diversas técnicas de confecção de modelo experimental de sinusite inflamatória em coelhos

Abstract

Experi mental models for clinical studies of rhinosinusitis are needed. Aim: to define a reliable, solid and reproducible experimental model for inflammatory rhinosinusitis with no innoculation of infectious agents in rabbits. Study design: Experimental. Material and Method: Twenty 20 rabbits were divided into 4 groups submitted to 4 different interventions: the placement of a unilateral nasal fossa sponge, unilateral obliteration of the nasal ostium with cyanoacrylate, unilateral placement of antigens in the maxillary cavity and unilateral placement of blood in the maxillary cavity. The animals were monitored for 15 days and then anesthetized and sacrificed; the maxillary sinuses were evaluated histologicaly and results were compared with controls and between the intervention groups. Conclusion: Sponje and glue as agents of meatal obstruction and toxoid aplied in the antrum are efficient as methods for rhinosinusitis modeling. Blood was not efficient in producing sinusitis.

Osteitis and mucosal inflammation in a rabbit model of sinusitis

“… 17 , 18 , 19 , 20 , 21 , 22 Among several methods described for rhinosinusitis induction, this study utilized the introduction of a sponge containing a toxoid agent into one of the nasal cavities of the animals. In addition to being a technically simple and efficient procedure to produce sinusitis in 100% of animals in several studies, 22 , 23 it causes little injury to the nasal mucosa and no sinus injury.…”

“…Many of these micro-organisms are opportunistic pathogens of the respiratory and digestive system of rabbits, which after the prolonged course of rhinosinusitis and the resulting alterations in the upper respiratory tract, acquire the means to multiply and often replace the original infection-causing agent, as described by other authors. 23 , 25 …”

Evaluation of the ability of an experimental model to induce bacterial rhinosinusitis in rabbits

“…Some authors performed the inoculation of the infectious agent inside the maxillary sinus 7 , 22 , 25 , 26 , 28 , 29 and others, inside the nasal cavity. 15 , 20 , 30 The authors chose not to use the first technique, as it is more invasive and causes iatrogenic alterations in the sinus mucosa.…”

“…Other animals used for research are the Wistar and Sprague–Dawley rats, guinea pigs, and sheep. 5 , 6 , 7 …”

Ефективність І Переносимість Енісаміуму Йодиду Назального Спрею: Результати Доклінічних Та Клінічних Досліджень

“…Експериментальний риносинусит у кролів був індукований у 1-й день дослідження шляхом тампонади правої половини носової порожнини під загальною анестезією (кетамін/ксилазин -35/5 мг/кг, внутрішньом'язово) [21], що є добре відомою моделлю риносинуситу (РС) в експериментальній ринології [22,23]. Через 15 діб після відтворення патології у кролів вилучали тампони та проводили контроль наявності РС за допомогою назальної ендоскопії [24][25][26].…”