Structural and morphological features of Piaractus mesopotamicus (Holmberg, 1887) gills

Severi, William; Rantin, Francisco Tadeu; Fernandes, Manuel

doi:10.1590/s0034-71082000000300014

Cited by 11 publications

(4 citation statements)

References 16 publications

(27 reference statements)

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“…Por outro lado, já que as lamelas da região basal estão posicionadas mais distalmente do ambiente aquático externo e de forma mais proximal da água que entra pela faringe do animal, possivelmente sua maior extensão nos animais do T 4 seja explicada pelo fato de terem uma maior responsabilidade na captação do pouco O 2 existente na água logo que era ingerida. Isso é corroborado pelo fato de que o fluxo de água, através das diferentes porções do filamento (basal, intermediária e apical) varia de acordo com o volume ventilatório do animal, e sob ventilação moderada existe uma grande resistência para o fluxo na ponta do filamento (Severi et al 2000). Por isso as lamelas localizadas perto da ponta do filamento tendem a ser mais curtas que aquelas da base, fato este que explica o encontrado nos animais do T 4 .…”

Section: Discussionunclassified

Alterações do epitélio branquial e das lamelas de tilápias (Oreochromis niloticus) causadas por mudanças do ambiente aquático em tanques de cultivo intensivo

et al. 2009

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The behavior of the gill epithelium of tilapias cultured in tanks at different altitudes and interconnected with PVC pipes was analyzed. Gill filaments of four specimens from four tanks (T 1 , T 2 , T 3 e T 4 ) sequentially interconnected were submitted to histological routine to obtain 5-mm-thick cuts that were stained with HE or submitted to histochemistry reactions PAS + diastase solution or Alcian Blue pH 2.5 or Alcian Blue pH 1.0. Considering the intermediary, apical and basal regions of the filaments, the lamellar area was measured and the amount of mucous cells was counted. It was verified that oxygen, pH, and temperature decreased progressively as the water flew from one tank to another. Thus, an increase was realized of the amount of mucous cells and the lamellar area in T 2, as well as a progressive decrease of these measures on the tanks which received water from T 2 . Moreover, detachment of the gill epithelium, cellular hyperplasia in the interlamellar space, and telangectasias were observed in the fishes from T 2 , T 3 e T 4. It was concluded that the aquatic environment in tanks sequentially interconnected with PVC pipes suffers alterations from one tank to another, as that physico-chemical fluctuations reflect on the behavior of the gill epithelium through variations of the lamellar area and the amount of mucous cells.

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Section: Discussionunclassified

Alterações do epitélio branquial e das lamelas de tilápias (Oreochromis niloticus) causadas por mudanças do ambiente aquático em tanques de cultivo intensivo

et al. 2009

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“…The pacu (P. mesopotamicus) gills consist of four branchial arches, supported by two rows of primary lamellae, which are covered with stratified epithelium. The secondary lamellae consist of epithelial, lining, pillar, chloride, and mucous cells ( Figure 1A), as described by Severi et al, (2000). The observed histological changes are described below according to each concentration (Figure 1), on Table 4.…”

Section: Gillsmentioning

confidence: 91%

Sensitivity, ecotoxicity and histopathological effects on neotropical fish exposed to glyphosate alone and associated to surfactant

Cruz¹,

Shiogiri²,

Silva³

et al. 2016

J. Environ. Chem. Ecotoxicol.

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The aim of this research was to evaluate neotropical fish sensitivity (Piaractus mesopotamicus, Phallocerus caudimaculatus, Hyphessobrycon eques, and Brachydanio rerio) to a reference substance (potassium chloride); to estimate the lethal concentration (LC 50 ; 96 h) for glyphosate, formulated as Rodeo ® alone and in association with 0.5 and 1.0% Aterbane ® BR surfactant and to evaluate the histopathology of the gills, liver, and kidney from the fish after acute exposure. P. caudimaculatus and H. eques are good bioindicators like B. rerio because they have similar sensitivity. The LC 50 ;96 h for glyphosate alone and in association with 0.5% Aterbane ® BR was similar (>975.0 mg L-1) for all the fish. Aterbane ® BR alone was the most toxic substance to P. caudimaculatus (5.81 mg L-1 LC 50 ;96 h) and glyphosate associated to 1.0% Aterbane ® BR was more toxic to H. eques (411.91 mg L-1 LC 50 ;96 h). The glyphosate alone and in association with Aterbane ® BR was classified as practically non-toxic, whereas Aterbane ® BR alone was considered moderately toxic for the tested organisms. The histopathological effects caused by glyphosate exposure on gills, liver, and kidneys are reversible, except for the liver necrosis on P. caudimaculatus. H. eques, P. caudimaculatus, and P. mesopotamicus present great potential to be used as standard organisms for herbicides monitoring and the use of glyphosate without surfactant addition is enough to cause histological alterations on H. eques and P. caudimaculatus, which makes them possible to be applied on environmental monitoring studies as biomarkers.

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“…We have applied an innovative approach by using lectins as probes for identification of mycobacteria in Piaractus mesopotamicus (Pacu), a bony fish that is endemic to the Paraguay-Paraná River basin and that has been introduced by aquaculture activities into a wider area (15).…”

Section: Introductionmentioning

confidence: 99%

Lectinhistochemical staining of granuloma induced by bacillus Calmette-Guerin in Piaractus mesopotamicus

et al. 2013

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Objetive. This study was conducted to evaluate, by means of lectinhistochemistry (LHC), the expression of carbohydrates in granulomas induced by the bacillus Calmette-Guerin (BCG) in muscle tissue of Piaractus mesopotamicus after 33 days. Material and methods. Histological sections with 3 μm thick were incubated with the following lectins :WGA (Wheat germ agglutinin), DBA (Dolichos biflorus agglutinin) and HPA (Helix pomatia agglutinin), and the results were evaluated by light microscopy. Results. Acid fast bacilli were stained by Ziehl Neelsen (ZN) and strong labeled by WGA in the cytoplasm of macrophages. Labeling with DBA was intense in fibroblasts and weak in macrophages. On the other hand, HPA binding was stronger in macrophages, especially in those that were in close contact with epithelioid cells, without evidence of binding to fibroblasts. The epithelioid cells were not labeled by the used lectins, but they were identified by Hematoxilin-Eosin (HE). The lectins labeled specific type saccharides in glycoproteins, as N-acetylglucosamine present in bacilli and macrophages, as well as N-acetyl-galactosamine in macrophages. The control group showed no inflammation or lectin binding. Conclusions. This technique may be useful in identifying receptors for WGA, DBA and the HPA lectins in epithelioid granuloma induced by BCG in P. mesopotamicus

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Structural and morphological features of Piaractus mesopotamicus (Holmberg, 1887) gills

Cited by 11 publications

References 16 publications

Alterações do epitélio branquial e das lamelas de tilápias (Oreochromis niloticus) causadas por mudanças do ambiente aquático em tanques de cultivo intensivo

Alterações do epitélio branquial e das lamelas de tilápias (Oreochromis niloticus) causadas por mudanças do ambiente aquático em tanques de cultivo intensivo

Sensitivity, ecotoxicity and histopathological effects on neotropical fish exposed to glyphosate alone and associated to surfactant

Lectinhistochemical staining of granuloma induced by bacillus Calmette-Guerin in Piaractus mesopotamicus

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