Mitogenic activity of fetal bovine serum, fish fry extract, insulin-like growth factor-I, and fibroblast growth factor on brown bullhead catfish cells - BB line

Abstract

Bioassays were performed to assess the effects of different levels of growth medium supplementation with fetal bovine serum (FBS), fish fry extract (FE), combinations of FBS and FE, and addition of insulin-like growth factor I (IGF-I) and fibroblast growth factor (FGF) on the proliferation of brown bullhead catfish cells (BB line). Treatments (n = 4) were: 2.5, 5, 10, and 15.0% FBS or FE and 5/2.5, 5/5, 10/2.5, and 10/5 of a FBS/FE combination as supplement to the growth medium, or the addition of 0.1, 1, 2.5, 10, 25, and 75 ng/ml of either IGF-I or FGF to the growth media. Initial cell density was 1.1 x 10(6) cells per well on uncoated 24-well plates. Incubation temperature was 29.5 +/- 0.7 degrees C. Six hours after plating, initial culture medium was removed, plates rinsed with Dulbecco's phosphate buffered saline, treatment media added, and cells allowed to proliferate for 24 hours. Another bioassay was performed with rat myoblast omega cells (RMo) using the same levels of growth medium supplemented with FBS, FE and FBS/FE. Base growth medium was Dulbecco's MEM. The initial cell density was 7.2 x 10(6) cells per well, and the bioassay was carried out at 36.0 +/- 0.5 degrees C, on a 95% air, 5% CO2 incubator. Increasing levels of FBS had a positive effect (P < 0.05) on the proliferation of both BB and RMo cells. Increasing levels of FE had a negative effect (P < 0.05) on the proliferation of BB cells and totally inhibited the proliferation of RMo cells at any level of supplementation. Higher levels of FE on the FBS/FE combinations presented a negative effect on the proliferation of both BB and RMo cells (P < 0.05). Insulin-like growth factor I had a positive quadratic effect (P < 0.05) on the proliferation of BB cells. Apparently, mammalian growth factors slightly stimulated mitogenic activity in fish cells, while FE contained factors which inhibited the mitogenic activity of RMo and BB cell lines.

Derivation and characterization of embryonic stem‐like cells of Indian major carp Catla catla

“…FGF proteins have diverse roles during development by interacting with bone morphogenetic proteins (BMP) (Naski & Ornitz, 1998; Delaune et al , 2005). In most of the cultured cells, basic FGF is a potent mitogen and an inhibitor of differentiation in mammalian primordial germ cells (PGCs) and ES cells (Matsui et al , 1992; Cyrino & Mulvaney, 1999; Levenstain et al , 2006).…”

“…The presence of LIF and its receptor in fishes has been reported earlier (Abe et al , 2007; Hanington et al , 2008). Another mitogen, insulin‐like growth factor (IGF1), neither stimulated mitosis nor the self‐renewal activity of CCES cells that may be attributed to its late expression during development (organogenesis) for specific differentiation events (Cyrino & Mulvaney, 1999; Zou et al , 2009).…”

Cellular activity and development of enteric, hepatic and muscle tissues of juvenile pacuPiaractus mesopotamicus(Holmberg 1887) fed with lyophilized bovine colostrum

“…In addition, muscle growth presented during the trial period can be related to endogenous IGF‐1 concentrations. According to Cyrino and Mulvaney (), mammalian growth factors stimulated mitogenic activity in a fibroblastic‐like cell line (BB line) from brown bullhead catfish Ictalurus punctatus cells in vitro . Castillo, Codina, Martínez, Navarro and Gutiérrez () and Li, Lou, Zhai, Peng, Cheng, Daí, Zhuo, Shang, Jin, Chen, Han, He and Yin () also support the idea that IGF‐1 stimulates cell proliferation, the mitogenic effects in skeletal muscle, promotes myofibre hyperplasia, cellularity changes and differentiation of rainbow trout Oncorhynchus mykiss and crucian carp Carassius auratus muscle cells in vitro and in vivo .…”

Basic Techniques and Limitations in Establishing Cell Culture: a Mini Review

“…So, they have to be added in the media for survival, growth, and proliferation to promote in vitro proliferation and differentiation of specialized cells. Over the years, considerable attention has been devoted to the role of peptide growth factors in the regulation of poultry and mammalian cell growth (Cyrino and Mulvaney, 1999). Notable major peptide growth factors (GF) are fibroblast GF, epidermal GF, platelet-derived GF, vascular Endothelial GF, Insulin GF (IGF-1, IGF-2) and transforming GF (Florini and Magri, 1989;Ewton and Florini, 1990;Florini et al, 1991).…”