Propagação de mudas de helicônia em biorreator de imersão temporária

Rodrigues, Paulo Hercílio Viegas; Teixeira, Fabiano M.; Lima, Ana Maria Liner Pereira; Ambrosano, Gláucia María Bovi

doi:10.1590/s0006-87052006000100005

Cited by 14 publications

(7 citation statements)

References 9 publications

(7 reference statements)

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“…Na cultura de helicônia, intervalos menores entre as imersões promoveram o desenvolvimento e a propagação dos explantes em biorreator de imersão temporária (Rodrigues et al, 2006). A variação do tempo de imersão e sua freqüência devem ser determinadas de acordo com as exigências de cada cultivo.…”

Section: Resultsunclassified

Métodos de micropropagação de abacaxizeiro

Silva

Pasqual

Teixeira

et al. 2007

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi comparar diferentes métodos de micropropagação in vitro de abacaxizeiro em larga escala. Os tratamentos utilizados foram micropropagação convencional em meio sólido (5 e 6 g L -1 de ágar) e em meio líquido, e micropropagação em biorreator de imersão temporária -imersão no meio de cultura a cada 2, 4 e 8 horas, por 3 min -e contínua, com aeração a cada 2, 4 e 8 horas, por 3 min. Utilizou-se meio MS suplementado com 1 mg L -1 de BAP, 0,25 mg L -1 de ANA, pH ajustado para 5,8. As culturas foram mantidas em sala de crescimento com 25±1 o C, sob luz branca fria (40 µmol m -2 s -1 ), com 16 horas de fotoperíodo. O delineamento foi inteiramente casualizado, com nove tratamentos e quatro repetições. Depois de 45 dias de cultivo, foram avaliados número de brotos, brotações superiores a 1 cm, comprimento de brotos, massa de matéria fresca e massa de matéria seca de brotos. Sistemas de imersão temporária, com as plântulas imersas a cada 2 horas por 3 min, proporcionaram maior número, altura e massa de matéria seca de brotos de abacaxizeiro. O sistema de imersão temporária é o método mais eficiente na micropropagação de abacaxizeiro em larga escala.Termos para indexação: Ananas comosus, propagação vegetal, imersão temporária. Micropropagation methods of pineappleAbstract -The objective of this work was to compare different methods of pineapple in vitro mass micropropagation. The treatments were conventional micropropagation in solid medium (5 and 6 g L -1 agar) and in liquid medium, micropropagation in bioreactor of temporary immersion (immersion in culture medium each 2, 4 and 8 hours, for 3 minutes) and continuous immersion (with aeration each 2, 4 and 8 hours, for 3 minutes). MS medium supplement with 1 mg L -1 BAP, 0.25 mg L -1 NAA, and pH adjusted to 5.8 was utilized. Cultures were incubated at 25±1 o C under cool white fluorescent tubes (40 µmol m -2 s -1 ) with a 16-hours photoperiod. The experiment design was completely randomized, comprising nine treatments and four replicates. After 45 days, number of shootings, shootings higher than 1 cm, length of shootings, fresh matter of shootings and dry matter of shootings were evaluated. Temporary immersion system in each 2 hours for 3 minutes provided higher number, height and dry matter of pineapple shootings. Temporary immersion system is the most efficient method in pineapple mass micropropagation.

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Section: Resultsunclassified

Métodos de micropropagação de abacaxizeiro

Silva

Pasqual

Teixeira

et al. 2007

Pesq. agropec. bras.

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“…However, the complex and expensive construction is the main argument against this design. Several low‐cost TIS, operating on the same principle, such as thermo‐photo‐bioreactors, have been developed by using glass bottles or NALGENE filtration systems . However, none of them can compete with the precise temperature control of thermo‐photo‐bioreactor TIS.…”

Section: Design and Operation Of Tismentioning

confidence: 99%

Temporary immersion systems in plant biotechnology

Georgiev

Schumann

Pavlov

et al. 2014

Engineering in Life Sciences

146

111

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Plant tissue and organ cultures in vitro usually face technological challenges. When submerged cultivation of plant cells in a controlled environment is desired, the characteristic growth morphology and physiology of differentiated organ cultures present a problem in process scale‐up. Temporary immersion systems (TIS) were developed several decades ago. These systems are providing the most natural environment for in vitro culture of plant shoots and seedlings. Over the past few years, TIS have been recognized as a perspective technology for plant micropropagation, production of plant‐derived secondary metabolites, expression of foreign proteins, and potential solutions in phytoremediation. Nowadays, several TIS, operating on similar or divergent technological principles, have been developed and successfully applied in the cultivation of various plant in vitro systems, including somatic embryos and transformed root cultures. In this article, the operational principle and technological design of the most popular TIS are reviewed. In addition, recent examples of the application of temporary immersion technology for in vitro cultivation of plant tissue and organ cultures at laboratory and pilot scales are discussed. Finally, future prospects and challenges to the industrial realization of that fast‐developing technique are outlined.

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“…The research work has been carried out exclusively under a photoperiod of 16 hours of light, generating good results, such as the increase in the number of shoots in Heliconia stricta Huber in a medium with 2.0 mg L -1 BAP (DINIZ et al, 2004); the study of somaclonal variation to promote such features as the reddish tone of the pseudostem in Heliconia bihai cv. Lobster Claw, that appears in the form of an equidistant spiral giving an exotic look to the flower stem (RODRIGUES et al, 2006); the in vitro regeneration of Heliconia psittacorum (GUZMÁN et al, 2009); the propagation of Heliconia curtispatha P (ALARCÓN et al, 2011); and the propagation of Heliconia psittacorum (NATHAN et al, 1992), among others.…”

Section: Resultsmentioning

confidence: 99%

“…In most of the studies carried out with different species of heliconia, the photoperiod used was 16 hours (NATHAN et al, 1992;DINIZ et al, 2004;RODRIGUES et al, 2006;GUZMAN et al, 2009;ALARCÓN et al, 2011), however the authors did not evaluate the effect of photoperiod on the in vitro micropropagation of the species (BRAGA et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Indução de brotações in vitro de Heliconia latispatha em função do fotoperíodo e reguladores de crescimento

Rodrigues

Santos

Carvalho

2016

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Considering the growing economic importance of tropical flowers and the advantages of techniques applied to the in vitro cultivation of these plants, it is necessary to carry out studies to evaluate growth in species such as Heliconia latispatha. The aim of this study therefore, was to evaluate in vitro shoot induction for different concentrations of BAP and NAA and as a function of the photoperiod. Explants from zygotic embryos were inoculated in MS medium containing different concentrations of BAP (0.0, 0.5, 1.0, 1.5, 2.0 or 2.5 mg L -1 ), with the cultures kept in a growth room at a temperature of 24.0 ± 2.0° C, under a photoperiod of 12 and 16 hours of light and a light intensity of 30 μmol m -2 s -1 . At 21, 28, 35, 42 and 49 days after inoculation, the number of shoots per explant was evaluated. The treatment at the BAP concentration that gave the best multiplication rate (2.5 mg L -1 ) was set, and was tested in a further trial with different concentrations of NAA (0.0, 0.2, 0.4, 0 6, 0.8 or 1.0 mg L -1 ) under the same conditions as the previous experiment. The experimental designs were completely randomised, with five replications, and analysed in a 6 x 2 factorial. The data were submitted to analysis of variance and regression. No significant differences were seen in relation to the photoperiod or its interaction with the cytokinin and auxin under test. Multiplication was greater in the presence of 2.5 mg L -1 BAP, which gave a rate of 1.25 shoots/explant at 49 days of in vitro culture. The association of this BAP dosage with 1.0 mg L -1 NAA was even more efficient, producing 1.83 shoots per explant at 30 days of growth. The use of BAP together with NAA is beneficial to the induction of shoots in H. latispatha. Keywords: Heliconiaceae, 6-benzylaminopurine, naphthaleneacetic acid, tissue culture. RESUMO Indução de brotações in vitro de Heliconia latispatha em função do fotoperíodo e reguladores de crescimentoConsiderando a crescente importância econômica das flores tropicais e as vantagens das técnicas aplicadas ao cultivo in vitro para estas plantas, torna-se necessária a realização de estudos que avaliem a propagação de espécies como a Heliconia latispatha. Diante do exposto, o objetivo do trabalho foi avaliar a indução de brotações in vitro em função de diferentes concentrações de BAP e ANA em função de fotoperíodos. Os explantes, oriundos de embriões zigóticos, foram inoculados em meio MS contendo diferentes concentrações de BAP (0,0; 0,5; 1,0; 1,5; 2,0 ou 2,5 mg L -1 ), mantendo-se as culturas em sala de crescimento com temperatura de 24,0 ± 2,0 ºC sob fotoperíodo de 12 e 16 horas de luz e intensidade luminosa de 30 μmol m -2 s -1 . Aos 21, 28, 35, 42 e 49 dias após a inoculação, foi avaliado o número de brotos obtidos por explante. O tratamento com a concentração de BAP que proporcionou melhor taxa de multiplicação (2,5 mg L -1 ) foi fixado e, em outro ensaio foi testado com diferentes concentrações de ANA (0,0; 0,2; 0,4; 0,6; 0,8 ou 1,0 mg L -1 ) nas mesmas condições do experimento ...

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Propagação de mudas de helicônia em biorreator de imersão temporária

Cited by 14 publications

References 9 publications

Métodos de micropropagação de abacaxizeiro

Métodos de micropropagação de abacaxizeiro

Temporary immersion systems in plant biotechnology

Indução de brotações in vitro de Heliconia latispatha em função do fotoperíodo e reguladores de crescimento

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