Morphoquantitative study of the submucous plexus (of Meissner) of the jejunum-ileum of young and old guinea pigs

Zanesco, Marcelo César; Souza, Romeu Rodrigues de

doi:10.1590/s0004-282x2011000100017

Cited by 6 publications

(5 citation statements)

References 13 publications

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“…In contrast, in the young group, we noticed a predominance of type Ⅲ collagen fibers, which were thinner and were characterized as immature collagen, which produces flexibility in the tissue. In accordance with the results observed [38] , the elderly group exhibited a replacement of type Ⅲ collagen with type Ⅰ collagen around the submucosal plexus of the jejunum and ileum compared to the young group. This finding suggests that changes in the distribution of collagen fibers could damage the function of the submucosal ganglia.…”

Section: Discussionsupporting

confidence: 90%

“…These results do not elucidate all the aspects of ICJ function; additional studies should be conducted in the future. the authors reported that in the submucosal plexus of the small intestine of rats at different ages, mitochondria were present in greater numbers in the elderly group and had a degenerative aspect [38] . In the process of aging, changes in cell morphology occur that include the presence of pleomorphic mitochondria [39] .…”

Section: Discussionmentioning

confidence: 98%

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Effects of aging on the architecture of the ileocecal junction in rats

Brito

Chopard

Cury

et al. 2016

WJGPT

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AIM:To evaluate the structural organization of the elastic and collagen fibers in the region of the ileocecal transition in 30 young and old male Wistar rats. METHODS:Histology, immunohistochemistry (IHC), transmission electron microscopy and scanning electron microscopy were employed in this study. The results demonstrated that there was a demarcation of the ileocecal region between the ileum and the cecum in both groups. RESULTS:The connective tissue fibers had different distribution patterns in the two groups. IHC revealed the presence of nitric oxide synthase, enteric neurons and smooth muscle fibers in the ileocecal junctions (ICJs) of both groups. Compared to the young group, the elderly group exhibited an increase in collagen type Ⅰ fibers, a decrease in collagen type Ⅲ fibers, a decreased linear density of oxytalan elastic fibers, and a greater linear density of elaunin and mature elastic fibers. CONCLUSION:The results revealed changes in the patterns of distribution of collagen and elastic fibers that may lead to a possible decrease in ICJ functionality. Core tip: The ileocolonic sphincter controls the forward and backward flow by integrating its motility with that of the distal ileum and proximal the ileocolonic sphincter controls the forward and backward flow by integrating its motility with that of the distal ileum and proxima. The ileocecal junction (ICJ) includes the muscle bundles in the terminal ileum, the intrinsic nerve plexus. The ICJ includes the muscle bundles in the terminal ileum, the intrinsic nerve plexus. Given the importance of knowing how the ICJ changes with age, the objective of this study was to characterize the morphological changes in the ICJ in rats aged 21 d and 2 years, using optical microscopy and electronic scanning and transmission methodologies. Additionally, the neurochemical characterization of the inhibitory neurons of the myenteric plexus, which are immunoreactive to the enzyme nitric oxide synthase, and the staining of the neuronal population were employed to identify immunoreactivity to HuC/D.de Brito MC, Chopard RP, Cury DP, Watanabe IS, Mendes CE, Castelucci P. Effects of aging on the architecture of the ileocecal junction in rats.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 98%

Effects of aging on the architecture of the ileocecal junction in rats

Brito

Chopard

Cury

et al. 2016

WJGPT

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“…However, the ENS appears to be more susceptible to age-related degeneration than other nervous systems (Saffrey, 2013). While some animal studies suggest that there may be reductions in the number of myenteric neurons in old age (El-Salhy et al, 1999; Phillips et al, 2004; Phillips and Powley, 2007; Zanesco and Souza, 2011), it is probable that myenteric neuron numbers are actually maintained, but an increasing proportion show structural degenerative changes with increasing old age (Gamage et al, 2013; Saffrey, 2013).…”

Section: Introductionmentioning

confidence: 99%

Colonic Motility and Jejunal Vagal Afferent Firing Rates Are Decreased in Aged Adult Male Mice and Can Be Restored by an Aminosterol

et al. 2019

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There is a general decline in gastrointestinal function in old age including decreased intestinal motility, sensory signaling, and afferent sensitivity. There is also increased prevalence of significant constipation in aged populations. We hypothesized this may be linked to reduced colonic motility and alterations in vagal-gut-brain sensory signaling. Using in vitro preparations from young (3 months) and old (18–24 months) male CD1 mice we report functional age-related differences in colonic motility and jejunal mesenteric afferent firing. Furthermore, we tested the effect of the aminosterol squalamine on colonic motility and jejunal vagal firing rate. Old mice had significantly reduced velocity of colonic migrating motor complexes (MMC) by 27% compared to young mice (p = 0.0161). Intraluminal squalamine increased colonic MMC velocity by 31% in old mice (p = 0.0150), which also had significantly reduced mesenteric afferent single-unit firing rates from the jejunum by 51% (p < 0.0001). The jejunal vagal afferent firing rate was reduced in aged mice by 62% (p = 0.0004). While the time to peak response to squalamine was longer in old mice compared to young mice (18.82 ± 1.37 min vs. 12.95 ± 0.99 min; p = 0.0182), it significantly increased vagal afferent firing rate by 36 and 56% in young and old mice, respectively (p = 0.0006, p = 0.0013). Our results show for the first time that the jejunal vagal afferent firing rate is reduced in aged-mice. They also suggest that there is translational potential for the therapeutic use of squalamine in the treatment of age-related constipation and dysmotility.

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“…However, isolated neurons were observed in smaller number. The predominance of neurons organized in ganglia was also observed in submucosal tela of rats' ileum (Buttow et al, 2004) and other species (Sousa, 1994;Zanesco & Souza, 2011).…”

Section: Discussionmentioning

confidence: 70%

Morphoquantitative Study of Rattus norvegicus Submucosal Plexus by Different Neuronal Evidentiation Histochemical Techniques

et al. 2016

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SUMMARY:Enteric nervous plexuses have been the object of several studies, specially the myenteric plexus whose studies describe its organization, functions and alterations. On the other hand, the submucosal plexus has been less studied and still needs descriptive studies. To analyze morphologically and quantitatively submucosal neurons of the jejunum of 90-day-old healthy rats using different techniques for neuronal staining as a way to provide normality data to compare with future experimental studies. Whole mount preparations of the jejunum were submitted to Giemsa, NADH-diaphorase and NADPH-diaphorase techniques to stain the total neuronal population, more metabolically active subpopulation and subpopulation of nitrergic neurons, respectively. Neurons of the submucosal plexus of adult rats are mainly organized in ganglia with varied sized and shapes. Giemsa technique stained 243.93 ± 7.68 neurons per mm 2 . Regarding the total population stained by Giemsa, NADH-diaphorase positive (139.09 ± 11.14/mm 2 ) neurons represented 57 % and NADPH-diaphorase positive (18.17 ± 0.28/mm 2 ) represented 7.5 %. The area of the cell body was bigger in nitrergic neurons (412.29 ± 150.22) than in the ones stained by Giemsa (254.71 ± 63.32) and NADH-diaphorase positive (243.98 ± 123.82).

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Morphoquantitative study of the submucous plexus (of Meissner) of the jejunum-ileum of young and old guinea pigs

Cited by 6 publications

References 13 publications

Effects of aging on the architecture of the ileocecal junction in rats

Effects of aging on the architecture of the ileocecal junction in rats

Colonic Motility and Jejunal Vagal Afferent Firing Rates Are Decreased in Aged Adult Male Mice and Can Be Restored by an Aminosterol

Morphoquantitative Study of Rattus norvegicus Submucosal Plexus by Different Neuronal Evidentiation Histochemical Techniques

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