Evaluation of the neuroprotective effect of ketoprofen on rats submitted to permanent focal brain ischemia

Silva, Manoel Nunes da; Colli, Benedicto Oscar; Coimbra, Norberto Cysne; Netto, Joaquim Coutinho

doi:10.1590/s0004-282x2007000600011

Cited by 6 publications

(4 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The large amount of glutamate accumulated in the extracellular space in the ischemic brain is probably due to glutamate release and impairment of its uptake (Rossi et al, 2000), with this high level of glutamate being thought to induce the hyperactivation of NMDARs followed by excess Ca 2+ influx into neurons, and cell death (Silva et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Analysis of the NMDA in Focal Cerebral Ischemia in Rats

et al. 2012

View full text Add to dashboard Cite

SUMMARY: NMDAR (N-methyl-D-aspartate receptor) is one subtype of ionotrophic glutamate receptor which is extensively distributed in the central nervous system (CNS). In the mammalian CNS, NMDAR serves prominent roles in the pathophysiologic process of cerebral ischemia. This study aimed to investigate the pattern of expression of protein and gene of the excitatory neurotransmitter NMDAR in experimental focal cerebral ischemia and the hole of neuroprotection with hypothermia and ketoprofen. 120 rats were randomly divided into 6 groups (20 animals each): control -no surgery; sham -simulation of surgery; ischemic -focal ischemia for 1 hour, without reperfusion; ischemic + intraischemic hypothermia; ischemic + previous intravenous ketoprofen, and ischemic + hypothermia and ketoprofen. Ten animals from each experimental group were used to establish the volume of infarct. Transient focal cerebral ischemia was obtained in rats by occlusion of the middle cerebral artery with an intraluminal suture. The infarct volume was measured using morphometric analysis of infarct areas defined by triphenyl tetrazolium chloride and the patterns of expression of the protein and gene NMDA were evaluated by immunohistochemistry and quantitative real-time PCR, respectively. Increases in the protein and gene NMDA receptor in the ischemics areas were observed and these increases were reduced by hypothermia and ketoprofen. The increase in the NMDA receptor protein and gene expression observed in the ischemic animals was reduced by neuroprotection (hypothermia and ketoprofen). The NMDA receptor increases in the ischemic area suggests that the NMDA mediated neuroexcitotoxicity plays an important role in cell death and that the neuroprotective effect of both, hypothermia and ketoprofen is directly involved with the NMDA. 980 (Dietrich et al.), has proved to be effective in providing protection after cerebral ischemia. Anti-inflammatory agents are used to attenuate the inflammatory response triggered by the ischemic phenomenon (Dietrich et al.), with emphasis on ketoprofen (Asanuma et al.; Dias et al.), a non steroid agent with a very potent analgesic and anti-inflammatory action, acting as a possible antagonist of the of the NMDA receptors (McCormack et al., 1994).One of the more intriguing reactions of cells to a severe metabolic insult is a change in their gene expression, which has become an important focus of interest in cerebral ischemia since several regulatory genes play an important role in the various aspects of infarct pathophysiology (Schneider et al., 2004). This study aimed to investigate the pattern of expression of the protein and gene NMDAR, in an experimental model of transient focal cerebral ischemia without reperfusion and the hole of neuroprotection with hypothermia and ketoprofen. MATERIAL AND METHODOne hundred and twenty adult male rats (Rattus norvegicus) weighing 280-310 g were used and the animals were randomly divided into 3 experimental groups: control (C), 20 animals sacrificed without being submitted to the surgic...

show abstract

Section: Discussionmentioning

confidence: 99%

Analysis of the NMDA in Focal Cerebral Ischemia in Rats

et al. 2012

View full text Add to dashboard Cite

show abstract

“…8 It exhibits similar therapeutic activity to different other nonsteroidal anti-inflammatory drugs (NSAID's) in managing dysmenorrhoea. 9 Permanent Focal Brain Ischemia Silva et al, 10 found that no neuroprotective response on the histopathological features or behavioural aspects of focal permanent brain ischemia during the study.…”

Section: Pharmacological Equivalence Studiesmentioning

confidence: 90%

Ketoprofen;

Zafar

Ali

Naqvi

et al. 2018

TPMJ

View full text Add to dashboard Cite

show abstract

“…Permanent Focal Brain Ischemia Silva et al, 10 found that no neuroprotective response on the histopathological features or behavioural aspects of focal permanent brain ischemia during the study. : White in 2006 11 reported a case study that child having heel pain had returned to his normal activities after few (18) days of intervention with ketoprofen gel.…”

mentioning

confidence: 84%