sICAM-1 in meningoencephalitis due to Angiostrongylus cantonensis

Dorta‐Contreras, Alberto Juan; Lewczuc, Piotr; Noris-García, Elena; Interián-Morales, Maria Teresa; Tarrau, María Esther Magraner; Padilla-Docal, Bárbara; Escobar-Pérez, X

doi:10.1590/s0004-282x2006000400011

Cited by 4 publications

(7 citation statements)

References 10 publications

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“…earlier published reports on sICAM-1 have been controversial due to less sensitive assays and unsuitable linear evaluation concepts for blood-CSF barrier dysfunction but later this problem was solved and several papers have confirmed the non-linear distribution of this protein according to its albumin quotients 2,3 .…”

Section: Discussionmentioning

confidence: 99%

“…sICAM-1 may be an agent in the negative feedback for eosinophils passage through the blood-CSF barrier into the inflammated brain in eosinophilic meningitis due to Angiostrongylus cantonensis. It has been suggested that a dynamic of the sICAM-1 brain derived fraction is perhaps associated to the immune response in the evolution of the disease 3 .…”

Section: Discussionmentioning

confidence: 99%

“…Routine CSF/serum analysis routine CSF/serum analysis was performed in all cases according to the protocol described earlier 3 . CSF samples contaminated with blood were not included in the study.…”

Section: Methodsmentioning

confidence: 99%

“…The curve was performed using the controls described previously paper 2,3 . The curve is based on data obtained from children and adults with control neurological diseases including patients with GBS.…”

Section: Routine Csf/serum Analysismentioning

confidence: 99%

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sICAM-1 intrathecal synthesis and release during the acute phase in children suffering from Coxsackie A9 and S. pneumoniae meningoencephalitis

Dorta‐Contreras

Lewczuk

Padilla-Docal

et al. 2008

Arq. Neuro-Psiquiatr.

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-The intercellular adhesion molecule is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. Serum and cerebrospinal fluid (CSF) soluble intercellular adhesion molecule 1 (sICAM-1) from normal control children as well as from children with Guillain-Barré syndrome (GBS), with Coxsackie A9 virus meningoencephalitis and with Streptococcus pneumoniae meningoencephalitis were studied. sICAM-1 was quantified using an immunoenzimatic assay and albumin using the immunodiffusion technique in both biological fluids. Increased sICAM-1 values in CSF in patients with GBS correspond to an increase of the albumin CSF/serum quotient. In contrast, in inflammatory diseases like S. pneumoniae and Coxsackie A9 virus meningoencephalitis an increased brain-derived fraction was observed. In particular cases these values are 60-65% and 70-75% respectively. The results indicate an additional synthesis of sICAM-1 in subarachnoidal space during central nervous system (CNS) inflammatory process. An important role of sICAM-1 in the transmigration of different cell types into CSF during CNS inflammation in children with S. pneumoniae and Coxsackie A9 meningoencephalitis may be suggested.Key wordS: cerebrospinal fluid, sICAM-1, Coxsackie A9, Streptococcus pneumoniae, children.Síntesis intratecal de sICAM-1 y liberación durante la fase aguda en niños con meningoencefalitis por Coxsackie A9 y S. pneumoniae Resumen -La molécula de adhesión intercelular es una glicoproteína que pertenece a la superfamilia de las inmunoglobulinas. Se estudiaron los niveles de molécula de adhesión intercelular tipo 1 soluble (sICAM-1) en suero y líquido cefalorraquídeo (LCr) de niños con meningoencefalitis por Streptococcus pneumoniae y por Coxsackie A9 al igual que en niños con sindrome de Guillain-Barré (SGB). sICAM-1 fue cuantificado por ensayo inmunoenzimático y la albúmina por inmunodifusión en ambos líquidos biológicos. Los valores incrementados de sICAM-1 en LCr en los pacientes con GBS corresponden a valores aumentados de razón LCr/suero de albúmina. en contraste, en las enfermedades inflamatorias como las meningoencefalitis por S. pneumoniae y por Coxsackie A9 se observa un incremento en la fracción derivada del cerebro. en casos particulares los valores se incrementan hasta un 60-65% y 70-75% respectivamente. Los resultados indican una síntesis adicional de sICAM-1 en el espacio subaracnoideo durante el proceso inflamatorio del sistema nervioso central (SNC). esto puede sugerir un importante papel del sICAM-1 en la transmigración de diferentes tipos celulares en el LCr durante la inflamación del SNC en niños con meningoencefalitis por S pneumoniae y coxsackie A9.PALABrAS-CLAve: líquido cefalorraquídeo, sICAM-1, Coxsackie A9, Streptococcus pneumoniae, niños.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Routine Csf/serum Analysismentioning

confidence: 99%

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sICAM-1 intrathecal synthesis and release during the acute phase in children suffering from Coxsackie A9 and S. pneumoniae meningoencephalitis

Dorta‐Contreras

Lewczuk

Padilla-Docal

et al. 2008

Arq. Neuro-Psiquiatr.

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“…1 The first report of this zoonosis was published in Cuba and in the Western Hemisphere more than 25 years ago. [2][3][4][5] If an infection occurs in non-permissive hosts, including humans and mice, development of parasites will terminate at the young adult worm stage in the brain and cause eosinophilic meningitis or meningoencephalitis. [6][7][8][9] Complement is a central component of the innate immune system comprising at least 35 proteins that collaborate in an intricate manner in the elimination of microorganisms and removal of apoptotic cells, and also serves as a natural adjuvant, enhancing and directing the adaptive immune response.…”

Section: Introductionmentioning

confidence: 99%

Reibergram of Intrathecal Synthesis of C4 in Patients with Eosinophilic Meningitis Caused by Angiostrongylus cantonensis

Padilla-Docal¹,

Dorta‐Contreras²,

Bu-Coifiu-Fanego³

et al. 2010

The American Society of Tropical Medicine and Hygiene

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Angiostrongylus cantonensis produces eosinophilic meningitis in humans and is endemic in Thailand, Taiwan, China, and the Caribbean region. During infection with this parasite, it is important to know if the complement system may be activated by the classical or lectin pathway. Cerebrospinal fluid and serum samples from 20 patients with meningitic angiostrongyliasis were used to quantify C4 levels and albumin. Results were plotted on a C4 CSF/serum quotient diagram or Reibergram. Twelve patients showed intrathecal synthesis of C4. Antibody-dependent complement cytotoxicity should be considered as a possible mechanism that destroys third-stage larvae of this helminth in cerebrospinal fluid of affected patients.

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Chip Electrophoresis as a Method for Quantifying Total Albumin in Cerebrospinal Fluid

Chan

Herold

2009

JALA: Journal of the Association for Laboratory Automation

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T he cerebrospinal/serum albumin ratio (Q Alb ) is a measure of cerebrospinaleblood barrier permeability, where dysfunctional barriers have been associated with a variety of disease states. Thus, the ability to accurately quantify albumin has critical diagnostic importance. The current work uses chip electrophoresis as an alternative methodology to the conventional immunoassay for quantifying cerebrospinal fluid (CSF) albumin. With variable concentrations of bovine serum albumin normalized to a chicken albumin internal standard, the electrophoresis system demonstrated signal correlation with a best-fit polynomial (R 2 ¼ 0.9993) (concentration range of 7.8e1000 mg/L). Interchip and intrachip variation studies conducted on patient CSF demonstrated coefficient of variations of 0.1e17%. Chip electrophoresis detected an average of 24% greater albumin than the immunoassay in patient CSF samples (n ¼ 58), indicating the ability to detect immunoreactive and non-immunoreactive forms of albumin. Finally, the correlation of CSF albumin concentration to Q Alb determined by chip electrophoresis (r 2 ¼ 0.8683) was comparable with that determined by immunoassay (r 2 ¼ 0.8390). Therefore, this work demonstrates proof-of-principle that chip electrophoresis can serve as an alternative method for quantifying total (immunoreactive and non-immunoreactive) albumin in CSF.

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sICAM-1 in meningoencephalitis due to Angiostrongylus cantonensis

Cited by 4 publications

References 10 publications

sICAM-1 intrathecal synthesis and release during the acute phase in children suffering from Coxsackie A9 and S. pneumoniae meningoencephalitis

sICAM-1 intrathecal synthesis and release during the acute phase in children suffering from Coxsackie A9 and S. pneumoniae meningoencephalitis

Reibergram of Intrathecal Synthesis of C4 in Patients with Eosinophilic Meningitis Caused by Angiostrongylus cantonensis

Chip Electrophoresis as a Method for Quantifying Total Albumin in Cerebrospinal Fluid

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