Simultaneous detection of Neisseria meningitidis, Haemophilus influenzae and Streptococcus sp. by polymerase chain reaction for the diagnosis of bacterial meningits

Abstract

Dra. Luciane Failace -Rua Carlos Von Koseritz 1353/201 -90540-031 Porto Alegre RS -Brasil. Acute bacterial meningitis is responsible for m o s t infections affecting the central nervous system ( C N S ) .Close to two-thirds of all cases occur among child ren, and Haemophilus influenzae, Neisseria meningitidis, and Streptococcus pneumoniae are the pathogens responsible for 80 to 90% of the case s 1 , 2 . A rapid and precise etiologic diagnosis of bacterial meningitis is essential to determine adequate t reatment, and it can also significantly reduce mortality and the risk for long-term sequelae 3 .In epidemiological terms, the diagnosis of bacterial meningitis may indicate the need for selective immu- PA L AV R A S -C H AVE: reação em cadeia da polimerase (PCR), meningite bacteriana, líquido cefalorr a q u i d iano (LCR).

Association of Meningococcal Phenotypes and Genotypes With Clinical Characteristics and Mortality of Meningitis in Children

“…22 PCR also allows genogrouping (serogroup prediction) of isolates and more rapid implementation of preventive measures as chemoprophylaxis and vaccination. [22][23][24] The rate of strains with reduced susceptibility to penicillin G is relatively high in our survey (41.6%) and we found a higher prevalence among isolates with the C:2b:P1.5,2 phenotype belonging to the ST-8 CC. 25 In another study, no association was found between reduced penicillin susceptibility and fatal outcome.…”

Acute bacterial meningitis among children under 5 years of age in Oman: a retrospective study during 2000-2005

“…The diagnosis of meningitis in children relies on clinical suspicion and correct interpretation of available laboratory tests including CSF culture. Alternative laboratory methods such as antigen detection and nucleic acid amplification should be integrated into routine laboratory procedures to improve the detection of microorganisms in CSF [14,15]. The sex distribution shows a male to female ratio of 1.4:1 and the mean age of involvement was found to be 8 months.…”

Meningitis in the National Hospital: VI national epidemiological sample

“…En la actualidad, las técnicas de biología molecular, como la PCR, se están utilizando ampliamente para el diagnóstico etiológico de las infecciones del SNC. En 1991, Kristiansen et al indicaron en los resultados especificidad y sensibilidad del 91% (12,(14)(15)(16) .…”