Análise comparativa de cortes de encéfalos humanos com coloração por três técnicas diferentes

Meneses, Murilo S.; Pedroso, Juan Carlos Montano; Fuzza, Rúbia Fátima; Milano, Jerônimo Buzetti

doi:10.1590/s0004-282x2004000200016

Cited by 8 publications

(9 citation statements)

References 10 publications

(8 reference statements)

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“…The staining method used was the Barnard, Robert, and Brown technique in accordance with Meneses et al (2004), which highlights the gray matter, and was described here for the first time in domestic cats. The brain structures in the cat were found to be smaller when compared to dogs, as described by Lecouteur et al (1983).…”

Section: Discussionmentioning

confidence: 99%

“…The brain was removed and immediately immersed in a 10% formaldehyde solution. At the Experimental Surgery Section of the Medicine School at University of São Paulo (USP) -Ribeirão Preto campus, the brain was sectioned into 4 mm transverse planes in a specific cast (Cat Brain Matrix, ASI Instruments 2mm, Warren Michigan, USA) for cat brain, and stained using the "Barnard, Robert, and Brown" technique (Meneses et al, 2004), which stains the gray matter in blue allowing for distinction between both nervous tissue types.…”

Section: Methodsmentioning

confidence: 99%

“…The first description of the use of CT in veterinary patients occurred during the 1980s, according to Ohlerth and Scharf (2007). This diagnostic modality is based on the use of x-rays, which can create slices of anatomical structures of the patient's body, forming transverse sections or three-dimensional images by volumetric reconstruction (Bertolini and Prokop, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Brain neuroimaging of domestic cats: correlation between computed tomography and cross- sectional anatomy

Nepomuceno

Zanatta

Chung

et al. 2016

Arq. Bras. Med. Vet. Zootec.

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Computed tomography of the brain is necessary as part of the diagnosis of lesions of the central nervous system. In this study we used six domestic cats, male or female, aged between one and five years, evaluated by Computed Tomography (CT) examination without clinical signs of central nervous system disorders. Two euthanized animals stating a condition unrelated to the nervous system were incorporated into this study. The proposal consisted in establishing detailed anatomical description of tomographic images of normal brain of cats, using as reference anatomical images of cross sections of the stained brain and cranial part, with thicknesses similar to the planes of the CT images. CT examinations were performed with and without intravenous iodinated contrast media for live animals. With one euthanized animal, the brain was removed and immediately preserved in 10% formalin for later achievement in cross-sectional thickness of approximately 4mm and staining technique of Barnard, and Robert Brown. The head of another animal was disarticulated in the Atlanto-occipital region and frozen at -20ºC then sliced to a thickness of about 5mm. The description of visualized anatomical structures using tomography is useful as a guide and allows transcribing with relative accuracy the brain region affected by an injury, and thus correlating it with the clinical symptoms of the patient, providing additional information and consequent improvement to veterinarians during the course of surgical clinic in this species.Keywords: feline, tomography, brain anatomy RESUMO A tomografia computadorizada do cérebro é de suma importância como parte do diagnóstico de lesões do sistema nervoso central. Neste estudo, foram utilizados seis felinos domésticos, machos ou fêmeas, com idade entre um e cinco anos, avaliados pela tomografia computadorizada (TC), sem sinais clínicos de distúrbios do sistema nervoso central. Dois cadáveres indicando a condição relacionada com o sistema nervoso foram incorporados ao presente estudo. A proposta consistiu na criação da descrição anatômica detalhada das imagens tomográficas de cérebro normal de gatos, usando como referência a imagens anatômicas de secções transversais do cérebro coradas, realizadas com espessuras similares aos planos das imagens tomográficas. Exames de TC foram realizados com e sem contraste iodado intravenoso para os animais vivos. Em um dos

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Brain neuroimaging of domestic cats: correlation between computed tomography and cross- sectional anatomy

Nepomuceno

Zanatta

Chung

et al. 2016

Arq. Bras. Med. Vet. Zootec.

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“…by local increases in the blood volume, flow and oxygenation [40]. This increase activates an intense magnetic field resulting from the alignment of its nuclear spins (orientation of subatomic particles when immersed in a magnetic field).…”

Section: Functional Magnetic Resonance Imagingmentioning

confidence: 99%

Neurorestorative Effects of Constraint-Induced Movement Therapy after Stroke: An Integrative Review

Lucas¹,

Ribeiro²,

Cagy³

et al. 2013

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Stroke has been considered as one of the main causes of death and of motor and cognitive sequels. Especially, many patients with upper limb hemiparesis improved their motor action and showed meaningful cortical changes after treatment with constraint-induced movement therapy. Therefore, this review aims to verify the literature about neuroimaging and behavioral evidences in the cortical reorganization through the use of the constraint-induced movement therapy. So, we conducted the literature research in indexed journals from many databases like Pubmed, Medline, Cochrane Database, Lilacs and Scielo. We concluded that the behavioral and neuroimaging studies using traditional and modified constraint-induced movement therapy promote cortical reorganization.

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“…(MENESES et al, 2004). Para auxiliar e complementar o estudo teórico é essencial a neuroanatomia prática, visando levar à compreensão e identificação dos componentes do sistema nervoso.…”

Section: Introductionunclassified

Comparação Entre Duas Técnicas De Coloração De Encéfalos Humanos

MERINI¹,

KRUM²,

COLMAN³

et al. 2014

Publ. Biologicas

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Data de recebimento: 18/11/2013 Data da aprovação: 21/01/2014 RESUMOO estudo da neuroanatomia de encéfalos humanos em cortes pode ser facilitado utilizando-se métodos de coloração para a substância cinzenta. O objetivo do estudo foi determinar, entre as técnicas de Mainland e de Barnard, Robert e Brown, qual teria melhores resultados para aplicação em laboratórios de anatomia. Foram testadas as técnicas em trinta cortes coronais de hemisfério cerebral, comparando entre si e analisando objetivamente de acordo com três parâmetros: grau de diferenciação entre as substâncias branca e cinzenta do córtex; presença de linha única e contínua separando a substância branca do córtex cerebral; grau de impregnação da coloração na substância branca. Depois de finalizada a coloração das peças, foi realizada uma análise objetiva da melhor visualização macroscópica da substância cinzenta. Foram atribuídas pontuações de 0 a 3 conforme a presença destes parâmetros, cada corte recebendo pontuação total que variava de 0 a 9. Após análise estatística, a técnica de Mainland apresentou resultado 6,13±1,64 e a de Barnard, Robert e Brown de 3,33±2,47 (p<0,001). Além desse resultado, em relação ao tempo dispendido para cada técnica e a facilidade do preparo de cada uma, o resultado a que chegamos concluiu que a técnica de Mainland apresentou-se como melhor opção quando comparada com a técnica de Barnard, Robert e Brown. Palavras-chave: Neuroanatomia. Técnicas de coloração. Encéfalo humano ABSTRACTThe neuroanatomy study of human brain can be facilitated by use of methods for staining of gray matter.The aim of this study was to determine, between staining techniques of Mainland and Barnard , Robert and Brown, which would be best for use in anatomy laboratories. The techniques were applied in coronal cerebral hemisphere, comparing

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Análise comparativa de cortes de encéfalos humanos com coloração por três técnicas diferentes

Cited by 8 publications

References 10 publications

Brain neuroimaging of domestic cats: correlation between computed tomography and cross- sectional anatomy

Brain neuroimaging of domestic cats: correlation between computed tomography and cross- sectional anatomy

Neurorestorative Effects of Constraint-Induced Movement Therapy after Stroke: An Integrative Review

Comparação Entre Duas Técnicas De Coloração De Encéfalos Humanos

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