Enzyme linked immunosorbent assay (ELISA) for the detection of IgG, IgM, IgE and IgA against Cysticercus cellulosae in cerebrospinal fluid of patients with neurocysticercosis

Abstract

-The objective of this study was to analyze different immunoglobulins classes (IgG, IgM, IgE and IgA) against Cysticercus cellulosae in the cerebrospinal fluid (CSF), through enzyme linked immunosorbent assay (ELISA), correlating them to clinical and tomographic profiles in patients with neurocysticercosis (NCC). Eighty-five specimens of CSF were obtained from 43 cases with NCC (26 with the active form and 17 with the inactive form) and from 42 patients with other neurological diseases. The inactive form of NCC presented a profile in CSF similar to the group without NCC. The active form of NCC presented elevation of specific immunoglobulins (IgG, IgM, IgE, and IgA) in decreasing order, with the highest values being detected among the cases with intraventricular cysts, or with inflammation signs in CSF or in those with multiple clinical manifestations. The highest sensitivity and specificity were obtained with ELISA-IgG (88.5% and 93.2%, respectively). This study confirmed the importance of ELISA in the immunologic diagnosis of NCC.

Evaluation of cysticercus-specific IgG (total and subclasses) and IgE antibody responses in cerebrospinal fluid samples from patients with neurocysticercosis showing intrathecal production of specific IgG antibodies

“…There was a marked discrepancy between the frequency of anti-cysticercal IgE antibodies detected here in CSF from patients with neurocysticercosis and that reported in the literature [17][18][19] . Variations in the immune response in neurocysticercosis are expected and are probably related to several factors, including heterogeneity of the patients included in the study, the immune status of the patients at the time of blood and/or CSF sample collection, the number, location and evolutionary stage of the parasites in the CNS, the intrinsic properties of the techniques used for antibody …”

“…Few studies have examined the simultaneous detection of cysticercus-specific total IgG and IgE in CSF samples from patients with neurocysticercosis [17][18][19] . In these studies, based on ELISAs standardized with crude cysticercal extracts, the sensitivities of the reactions for detecting IgG and IgE antibodies ranged from 11.8 to 100% and from 0 to 31.8%, respectively.…”

Dificultades diagnósticas y terapéuticas en la neurocisticercosis: presentación de 6 casos y revisión de la literatura

“…Dicho método mide los anticuerpos (AC) contra el cisticerco y tiene la ventaja de reconocerlo en aquellos casos con LCR no inflamatorio. La forma activa de NCC presenta una elevación de inmunoglobulinas (Ig) especificas (IgG, IgM, IgE, y IgA) en orden decreciente, con los valores más altos detectados entre los casos con quistes intraventriculares o signos inflamatorios en el LCR o aquéllos con manifestaciones clínicas múltiples 31 . La mayor sensibilidad y especificidad se obtiene con el ELISA-IgG (88.5% y 93.2% respectivamente) 31 .…”

“…La forma activa de NCC presenta una elevación de inmunoglobulinas (Ig) especificas (IgG, IgM, IgE, y IgA) en orden decreciente, con los valores más altos detectados entre los casos con quistes intraventriculares o signos inflamatorios en el LCR o aquéllos con manifestaciones clínicas múltiples 31 . La mayor sensibilidad y especificidad se obtiene con el ELISA-IgG (88.5% y 93.2% respectivamente) 31 . Los niveles de Ig dependen de la localización del quiste en el SNC 31 .…”

“…La mayor sensibilidad y especificidad se obtiene con el ELISA-IgG (88.5% y 93.2% respectivamente) 31 . Los niveles de Ig dependen de la localización del quiste en el SNC 31 . La sensibilidad en la detección de anticuerpos suele ser mayor en pacientes con quistes múltiples (94%) y, ostensiblemente menor, en pacientes con quistes únicos o calcificados 43 .…”