Alterations of cell-mediated immune response in children with febrile seizures

Montelli, Terezinha C. B.; Soares, Ana Flávia; Parise‐Fortes, Maria Rita; Rezkallah-Iwasso, M. T.; Padula, Niura Aparecida de Moura Ribeiro; Peraçoli, Maria Terezinha Serrão

doi:10.1590/s0004-282x1997000200005

Cited by 8 publications

(3 citation statements)

References 19 publications

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“…The results of lymphocyte proliferation in response to PHA stimulus showed a significant impairment of cell-mediated immunity when lymphocytes of patients were stimulated in vitro with the mitogen, in the presence of their own plasma, as detected by the low blastogenic index. These results as well as the reports obtained in other pathologies such as, febrile seizures 36 and infectious diseases 23,37 are compatible with the presence of inhibitory factors in patient plasma. The nature of these inhibitory factors has not been clarified yet, but some evidence indicate that antibodies, immune complexes, serum protein as alpha2-macroglobulin and parasite antigens may Naspitz, 1992 [25].…”

Section: Resultssupporting

confidence: 90%

Immunologic aspects of West syndrome and evidence of plasma inhibitory effects on T cell function

Montelli

Soares

Peraçoli

2003

Arq. Neuro-Psiquiatr.

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-Study objective: : :: : The purpose of this study was to assess the extent of immune dysfunction in a well-defined group of epileptic patients: children with diagnosis of West syndrome (WS) or with transitions to another age-related EEG patterns, the multifocal independent spikes (MIS), and the slow spike-wave complexes (Lennox-Gastaut syndrome -LGS). Thus, WS was studied at different points of the natural evolutive history of the disease. Method: A group of 50 patients (33 with WS, 10 with LGS and 7 with MIS) and 20 age-matched healthy controls were submitted to enumeration of T lymphocyte subsets: CD1, CD3, CD4, CD8, CD4/CD8 ratio and lymphocyte proliferation assay to phytohaemagglutinin (PHA), in the presence of autologous and AB, homologous plasma. Dinitrochlorobenzene (DNCB) skin test sensitization was performed only in patients. Determinations of IgG, IgA, and IgM serum levels were compared to standard values for Brazilian population in different age ranges. Results: Sensitization to DNCB showed absent or low skin reactions in 76% of the patients. High levels of IgG (45.7%) and IgM (61.4%), and lower levels of IgA (23.9%) were detected in the serum of the patients. Enumeration of lymphocyte subsets in peripheral blood showed: low CD3+ (p<0.05), low CD4+ (p<0.05), high CD8+ (p<0.01) and low CD4+ / CD8+ ratio (p<0.001). The proportion of CD1+ cells in the control group was less than 3%, while ranged between 6 and 11 % in 18% of the patients. The in vitro PHA-induced T cell proliferation showed significantly low blastogenic indices only when patients , cells were cultured in presence of their own plasma. No differences in blastogenic indices were observed when the cells of patients and controls were cultured with human AB plasma. Conclusion: The immunodeficiency in WS was mainly characterized by anergy, impaired cell-mediated immunity, altered levels of immunoglobulins, presence of immature thymocytes in peripheral blood and functional impairment of T lymphocytes induced by plasma inhibitory factors.KEY WORDS: dinitrochlorobenzene, West syndrome, Lennox-Gastaut syndrome, multifocal-independent spike syndrome, T-lymphocytes subsets, immunoglobulins, proliferation assay, T cells, CD1, CD3, CD4, CD8.Aspectos imunológicos da síndrome de W Aspectos imunológicos da síndrome de W Aspectos imunológicos da síndrome de W Aspectos imunológicos da síndrome de W Aspectos imunológicos da síndrome de West e evidência de efeitos inibitórios do plasma sobre a função est e evidência de efeitos inibitórios do plasma sobre a função est e evidência de efeitos inibitórios do plasma sobre a função est e evidência de efeitos inibitórios do plasma sobre a função est e evidência de efeitos inibitórios do plasma sobre a função de células T de células T de células T de células T de células T RESUMO -Objetivo: O objetivo deste estudo foi determinar o perfil da deficiência imune em um grupo bem definido de epilepsia: crianças com síndrome de West (SW) e seus padrões EEG de evolução, idade-dependentes, como os complexos onda-agud...

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Section: Resultssupporting

confidence: 90%

Immunologic aspects of West syndrome and evidence of plasma inhibitory effects on T cell function

Montelli

Soares

Peraçoli

2003

Arq. Neuro-Psiquiatr.

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“…Various other immunological parameters are also different in patients with FS. In a study by Montelli et al [8], an increase in CD8 + count, a low helper/supresor T lymphocytes ratio and a defective proliferative response to phytohemagglutinin in lymphocytes were detected in patients with FS. Based on literature data, some authors suggested that the balance between proinflammatory and antiinflammatory cytokines might have a role in the pathogenesis of FS [4].…”

Section: Introductionmentioning

confidence: 95%

Reduced cerebrospinal fluid levels of interleukin-10 in children with febrile seizures

Şahin

Uysal

Yentür

et al. 2019

Seizure

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A B S T R A C TPurpose: The exact etiology of febrile seizures (FS) is still unclear. However, it is thought that cytokine network activation may have a causative role. Therefore, this study aimed to evaluate the levels of interleukin-12 (IL-12) as a proinflammatory cytokine, interleukin-10 (IL-10) as an anti-inflammatory cytokine, and interferon-β (IFNβ), a marker of toll-like receptor-3 activation as a host response to viruses. These cytokine levels were analyzed in the cerebrospinal fluid (CSF) of children after a FS. Methods: With the approval of the Human Research Ethics Committee, 76 patients with FS, who underwent lumbar puncture (LP) for the exclusion of central nervous system (CNS) infection, and who didn't have CSF pleocytosis, were included in the study. The control group consisted of 10 patients with similar ages, with an acute febrile illness and who required LP to exclude CNS infection. The analyses were made by the enzymelinked immunoassay method. Results: Age, gender distribution and CSF IL-12 and IFN-β levels did not differ, but CSF IL-10 levels were significantly lower in the FS group as compared to the control group (0.78 ± 4.5 pg/ml, versus 27 ± 29 pg/ml, p < 0.0001). Conclusion: The low-level of CSF IL-10, considering its anti-inflammatory properties, may play a role in the etiopathogenesis of FS.

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“…Tartara et al 4 have recorded a high incidence of immunologic disorders in epileptic and febrile convulsive patients. Montelli et al 5 have stated that patients with FS have an impairment of cellular immunity. Aside from these, IgG subgroup deficiency and low/normal serum IgA levels in patients with FS have been recorded in several reports 6–11 …”

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confidence: 99%