Effects of maternal proteic undernutrition on the neurons of the myenteric plexus of the duodenum of rats

Natali, Maria Raquel Marçal; Miranda-Neto, Marcílio Hubner de

doi:10.1590/s0004-282x1996000200014

Cited by 41 publications

(28 citation statements)

References 12 publications

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“…Therefore, about 50.5% of the nitrergic neurons and 27.2% of the NADH-diaphorase positive neurons of the ascending colon were not evidenced. Significant reductions in the number of myenteric neurons of malnourished rats were also verified in other investigations, and have been attributed to the nutritional deficiency to which the animals were subjected [8][9][10][11]16 .…”

Section: Discussionsupporting

confidence: 53%

“…Experimental desnutrition is a fruitful area for the investigation of neuronal plasticity and the interdependence of neuronal and non-neuronal mechanisms 7 . Quantitative and morphological evaluations of the neurons of the myenteric plexus in malnourished rats have been demonstrating a larger neuronal density in the small intestine [8][9][10][11] and in the colon 12 , both due to the smaller growth of the animals and their bowels, which lead to lesser neuronal spreading. Most of the authors reviewed offered to the animals diets with protein deficiency but included minerals and vitamins of the complex B [8][9][10][11][12] .…”

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Effects of protein and vitamin B deficiency on blood parameters and myenteric neurons of the colon of rats

Sant’Ana

Molinari

Miranda-Neto

2001

Arq. Neuro-Psiquiatr.

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-The aims of this work were to evaluate the effects of the deficient ingestion of protein and vitamin B on the biochemical and hematologic parameters and on the NADH-and NADPH-diaphorase positive myenteric neurons. The control animals (n=10) received commercial chow and the experimental rats (n=10) received chow with protein level reduced to 8% during 120 days. At the time of killing blood was collected for assessment of the blood and hematologic parameters and the ascending colon for quantitative analysis of the neurons of the myenteric plexus. It was observed that the reduction of the protein level to 8% coupled to the reduction of the levels of vitamin B in adult rats neither led to qualitative or quantitative changes on red or white blood cells, nor decreased globulin levels, induced the formation of edema or gave rise to clinical signs typical of protein or vitamin B deficiency. On the other hand, the experimental protocol led to less weight gain, change on the body composition with fat deposition; decrease of the values of serum total protein and albumin; reduction of the area of colon and density of nitrergic and NADH-diaphorase myenteric neurons inferior to the expected.KEY WORDS: enteric neurons, proteic desnutrition, ascending colon, hematology, vitamin B.Efeitos da deficiência de proteínas e vitamina B sobre parâmetros sanguíneos e neurônios mioentéricos do colo de ratos adultos RESUMO -Os objetivos deste trabalho foram avaliar as repercussões da ingestão deficiente de proteínas e vitaminas do complexo B sobre parâmetros bioquímicos e hematológicos e neurônios mientéricos NADH e NADPH-diaforase positivos. Os animais controle (n=10) receberam ração comercial e os animais experimentais (n=10) ração com teor protéico reduzido para 8% durante 120 dias. Durante o sacrifício, coletou-se o sangue dos animais para avaliação de parâmetros sanguíneos e hematológicos e o colo ascendente para a análise quantitativa dos neurônios do plexo mioentérico. Observou-se que a redução do teor de proteínas na dieta para 8% associado a redução no teor de vitaminas do complexo B em ratos adultos, não causa alterações qualitativas e quantitativas das células sanguíneas das séries vermelha e branca, não leva a redução do valor da globulina nem a formação de edema ou ao surgimento de sinais clínicos característicos das carências de proteínas e vitaminas do complexo B. Por outro lado causa: menor ganho de peso corporal; alteração da composição corporal com acúmulo de gordura; redução nos valores de proteína total e albumina; redução na área do colo e evidenciação de densidade de neurônios nitrérgicos e NADH-diaforase positivos inferior ao esperado.PALAVRAS CHAVE: neurônios entéricos, desnutrição protéica, colo ascendente, hematologia, vitamina B. The utilization of animal models in studies of food restriction with known and controlled diets has as an advantage the improved knowledge about the many aspects of the human desnutrition 1 . Research on desnutrition aimsat simulating natural conditions, which involve deficienc...

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Section: Discussionsupporting

confidence: 53%

mentioning

confidence: 99%

Effects of protein and vitamin B deficiency on blood parameters and myenteric neurons of the colon of rats

Sant’Ana

Molinari

Miranda-Neto

2001

Arq. Neuro-Psiquiatr.

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“…It is also comparable to the studies of Natali et al (2003) that found 21757 neurons/cm 2 when quantified the myenteric neurons in duodenum using the same technique and rats with the same age as ours (210-day-old). The difference in the neuronal density found by Natali et al (1996) in 60-day-old control rats (31438 neurons/cm 2 ) and by Buttow et al (1997) in 135-day-old rats (30843 neurons/ cm 2 ), who also employed Giemsa's technique in duodenum, is due to the animals age.…”

Section: Discussionmentioning

confidence: 78%

Density and Morphometry of Overall Myenteric Neurons Population from the Duodenum of Diabetic Rats (Rattus norvegicus) under Ascorbic Acid Supplementation

et al. 2009

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SUMMARY:The objective of this work was to evaluate the overall myenteric neurons population from the duodenum of adult streptozotocin-induced diabetic rats supplemented with ascorbic acid (AA), a potent antioxidant. Fifteen 90-day-old rats were divided in groups: control (C), diabetic (D), diabetic treated with ascorbic acid (DA). After 120 days of experimental period duodenums were resected and processed as whole-mount preparations according to Giemsa's technique, which allowed us to evaluate neuronal density in an area of 8.96 mm 2 and measure the area of 500 neuronal cell bodies per group. It was observed a 32.55% reduction in neuronal density of group D when compared to group C (p<0.05). The density of spared neurons in group DA, in relation to group D, was not statistically different in this experimental model. No significant differences were found in neuronal areas when groups C and D or group D and DA were compared (p>0.05). These results lead us to conclude that the density of overall myenteric neurons population from the duodenum was reduced in diabetic rats (D), when compared to its control (C); and that diabetic rats supplemented with AA (DA) did not have their neuronal density preserved when compared to diabetic animals (D).

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“…In this study it was investigated whether the areas of neuronal cell body profiles showed variations according to the region of the intestinal circumference, because several authors, when carrying out studies of phylogenetic nature [11][12][13] or when assessing conditions such as desnutrition 14 , aging 9,10,15-17 and diabetes 2,8 , label the enteric neurons based on morphometric and quantitative analyses regardless of the region of the intestinal circumference. In some quantitative studies, the authors stress the existence of neuronal density differences according to the circumferential region of the digestive tube.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the areas of neuronal cell bodies and nuclei in the myenteric plexus of the duodenum of adult rats

Miranda-Neto

Furlan

Sant’Ana

et al. 2000

Arq. Neuro-Psiquiatr.

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-This study compared the areas of cell body and nucleus profiles of the myenteric neurons in the antimesenteric and intermediate regions of the duodenum of adult rats. Five male rats were used. The duodenum was removed and dissected to whole-mount preparations, which were stained by the Giemsa technique. The areas of cell body and nucleus profiles of 100 neurons, 50 from each region, of each animal, were assessed with image analyser. Based on the global mean±SD of the areas of cell body profiles, neurons were labelled as small, medium or large. It was observed that the neurons did not differ significantly in size or incidence between the antimesenteric and intermediate regions. However, the nuclei of the small and medium neurons were significantly smaller in the latter region. It is discussed that the smaller nuclear size could be related to the cell bodies being slightly smaller on this region and to a possible smaller biosynthetic activity which would influence nuclear size.KEY WORDS: myenteric neurons, neuronal area, duodenum, rats. Avaliação das áreas dos corpos celulares e dos núcleos neuronais no plexo mientérico do duodeno de ratos adultosRESUMO -Este estudo comparou as áreas dos perfis dos corpos celulares e dos núcleos neuronais dos neurônios mientéricos nas regiões antimesentérica e intermediária do duodeno de ratos adultos. Cinco ratos machos foram usados. O duodeno foi removido e dissecado a preparados de membrana, os quais foram corados pela técnica de Giemsa. As áreas dos perfis dos corpos celulares e dos núcleos neuronais de 100 neurônios, 50 de cada região, de cada animal, foram avaliadas com analisador de imagens. Com base na média global±SD das áreas dos perfis dos corpos celulares, os neurônios foram classificados como pequenos, médios ou grandes. Foi observado que os neurônios não diferiram significativamente de tamanho ou incidência entre as regiões antimesentérica e intermediária. Contudo, os núcleos dos neurônios pequenos e médios foram significativamente menores na última. Discute-se que o menor tamanho nuclear poderia estar relacionado ao fato dos corpos celulares serem ligeiramente menores nesta região e a uma atividade biossintética possivelmente menor, que influenciaria o tamanho nuclear. PALAVRAS-CHAVE: neurônios mientéricos, área neuronal, duodeno, ratos.For many years the enteric nervous system (ENS) has been the goal of studies aiming, first, at unravelling its intricate structure, neuronal cell types, connections and neurochemical coding and, second, at understanding how these aspects are affected by factors such as age and pathophysiological Trabalho resultante de pesquisa realizada durante o mestrado de MMDP Furlan pelo

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Effects of maternal proteic undernutrition on the neurons of the myenteric plexus of the duodenum of rats

Cited by 41 publications

References 12 publications

Effects of protein and vitamin B deficiency on blood parameters and myenteric neurons of the colon of rats

Effects of protein and vitamin B deficiency on blood parameters and myenteric neurons of the colon of rats

Density and Morphometry of Overall Myenteric Neurons Population from the Duodenum of Diabetic Rats (Rattus norvegicus) under Ascorbic Acid Supplementation

Evaluation of the areas of neuronal cell bodies and nuclei in the myenteric plexus of the duodenum of adult rats

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