-765 G>C POLYMORPHISM OF THE COX-2 GENE AND GASTRIC CANCER RISK IN BRAZILIAN POPULATION

Abstract

-Context -Genomic alterations play important roles in gastric cancer carcinogenesis. Cyclooxygenases (COX) are important enzymes in the maintenance of mucosal integrity and in pathological processes, mainly in inflammation and cancer. The -765G>C COX-2 polymorphism has been implicated in gastric cancer risk. Objective -To evaluate the COX-2 gene polymorphism as a predictor of gastric cancer risk. Methods -One hundred gastric cancer patients and 150 controls were enrolled from a Brazilian centre. Personal data regarding related risk factors, including alcohol consumption and smoking behavior, were collected via questionnaire. DNA was extracted from peripheral blood and the genotypes were analyzed using PCR-restriction fragment length polymorphism.Results -G/G, G/C and C/C genotypes frequencies was 42.7%, 50% and 7.3%, respectively in controls and 59.0%, 34.0% and 7.0% in gastric cancer. The frequency of the genotypes differed between the groups (P = 0.033). A higher risk of gastric cancer was associated with COX-2 -765G/G genotype (P = 0.048; OR:1.98, 95% CI = 1.01-3.90). Alcohol consumption and smoking in patients with -765G/G genotype also increased the risk of gastric cancer. Conclusion -The -765G/G genotype and the -765G allele had been associated with an increased risk for gastric cancer. The presence of smoking and alcohol consumption increased the risk for gastric cancer in subjects with -765G/G genotype compared with the control group. Polymorphism of COX-2 gene and gastric cancer risk HEADINGS -Polymorphism single nucleotide. Gastric cancer. Cyclooxygenase 2.

Association between rs20417 polymorphism in cyclooxygenase-2 and gastric cancer susceptibility

“…A total of 15 original papers that met the inclusion criteria were selected for the meta-analysis; they included an assessment of the association between COX - 2 rs20417 polymorphism and GC in 2848 cancer cases and 4962 healthy controls. [22–27,31–39] These papers reported studies of Asian (11 papers), [22–26,31,32,34–37] Caucasian (3 papers), [27,33,38] and American (1 paper) [39] (details shown in Table 1). The association between COX - 2 rs20417 polymorphism and GC susceptibility in H pylori positive and H pylori negative patients under the dominant model were available in 5 studies.…”

Mutational landscape of gastric adenocarcinoma in Latin America: A genetic approach for precision medicine

“…IL-8 c.-251A > T, IL-10 c-592C > A, and IL-10 c.-1082 A > G are the most studied germline risk variants, with GA susceptibility studies reported in countries such as Brazil, 39 , 40 , 41 , 42 , 43 Chile, 44 , 45 Colombia, 46 Mexico, 47 and Peru 48 ( Fig. 2 and Table 2 ).…”

Genetic polymorphisms as non-modifiable susceptibility factors to laryngeal cancer

“…Prostaglandin-endoperoxide synthase 2 (PTGS2) or Cyclooxygenase-2 (COX2) is an enzyme involved in inflammation and tissue damage responses, which could lead to cancer. Two SNPs rs20417 (c.-899G>C, C allele) and rs689466 (c.-1329A>G, A allele) increasing COX2 protein expression have been reported as potential susceptibility biomarkers [9][10][11][12][13][14]. On the other hand, epidermal growth factor receptor (EGFR), a tyrosine kinase receptor activated by the epidermal growth factor (EGF), is essential in laryngeal squamous epithelium differentiation, survival, and proliferation.…”