Evaluation of the immunoexpression of COX-1, COX-2 and p53 in Crohn's disease

Abstract

-Background -Crohn's disease accompanied by nonspecific or idiopathic ulcerative proctocolitis corresponds to a condition called intestinal inflammatory disease. The immunoexpression of cyclooxygenase 2 (COX-2) in Crohn's disease becomes more marked with progression of the disease and the presence of wild-type p53 suppresses the transcription of COX-2. Aims -To investigate the immunoexpression of cyclooxygenase 1 (COX-1), COX-2 and p53 in Crohn's ileocolitis and to correlated this expression with clinical and histopathological parameters. Methods -Forty-five cases of Crohn's disease, 16 cases of actinic colitis (diseased-control group) and 11 cases without a history of intestinal disease (normal control group) were studied. Hematoxylin-eosin-stained sections were submitted to histopathological analysis and the immunohistochemical expression of COX-1, COX-2 and p53 was evaluated by the streptavidin-biotin-peroxidase method. Results -Sixty percent of the Crohn's disease patients were women and 40% were men, with 75.5% whites and 25.5% non-whites. The disease involved the terminal ileum in 44.5% of cases, ileum in 33.3%, colon in 20% and duodenum-ileum in 2.2%. A significant association was observed between COX-2 immunoreactivity and age ≤40 years. Histopathological analysis of Crohn's disease samples showed mild or moderate crypt distortion (57.8% and 35.6% of cases), atrophy (6.6%), mild, moderate and marked chronic inflammation (46.7%, 26.7% and 20%), acute inflammatory activity (93.3%), ulceration (24.4%), mucin depletion (37.8%), Paneth's cells (24.4%), intraepithelial lymphocytes (93.3%), and subepithelial collagen (6.7%). In the CD group, COX-1 immunoreactivity in epithelial and inflammatory cells was observed in 26.7% and 22.2% of cases, respectively.

Novel anti-inflammatory functions for endothelial and myeloid cyclooxygenase-2 in a new mouse model of Crohn's disease

“…1 and 4). Previous reports demonstrated that COX-2 protein is induced in intestinal epithelial cells in CD patients (41,44). Our results suggest that COX-2 products from intestinal epithelial cells are not involved in cholate-mediated inflammation at the ileo-ceco-colic junction.…”

p53 expression in patients with ulcerative colitis - associated with dysplasia and carcinoma: a systematic meta-analysis

“…Second, the main ethnic groups consisted of Asians and Caucasians in this study, but other study populations, such as African population, were lacking. Finally, three eligible studies reported the data between p53 expression and dysplasia and cancer in patients with Crohn’s disease (CD) [ 37 , 43 , 44 ], more studies with large sample sizes are essential to further perform a meta-analysis to assess the correlation of p53 expression in CD in the future.…”

Role of different inflammatory and tumor biomarkers in the development of ulcerative colitis-associated carcinogenesis

“…In UC patients, several inflammation-associated genes such as COX-2, iNOS, or cytokine-inducible genes are also increased in inflamed mucosa. 52 It is generally observed that tumors, especially colitis-associated CRC, are usually infiltrated by activated immune cells that secrete proinflammatory cytokines, such as TNF-a, IFN-c, and IL-6. These molecules have been shown to have a crucial role in promoting neoplastic transformation.…”