Mast Cells Distinguish Eosinophilic Esophagitis in Pediatric Patients

Lomazi, Elizete Aparecida; Brandalise, Nelson Ary; Servidoni, Maria de Fátima; Cardoso, Sílvia Helena; Meirelles, Luciana Rodrigues de

doi:10.1590/s0004-2803.201700000-23

Cited by 7 publications

(6 citation statements)

References 28 publications

(40 reference statements)

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“…In addition, clinical studies demonstrated elevated mast cell number in oesophagus biopsies from EoE patients . Moreover, mast cell accumulation in the oesophageal mucosa can be used in the diagnosis, in order to discern EoE from GERD …”

Section: Discussionmentioning

confidence: 99%

High‐fat diet‐induced obesity worsens TH2 immune response and immunopathologic characteristics in murine model of eosinophilic oesophagitis

Silva

Oliveira

Ambrósio

et al. 2019

Clin Experimental Allergy

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Background: Eosinophilic oesophagitis (EoE) is an emergent chronic immunemediated disease of the oesophagus, which affects both children and adults. It is clinically characterized by dysphagia, food impaction and oesophageal eosinophilia.Epidemiological studies indicate that obesity can worsen allergic symptoms; however, its effect on EoE immunopathological response has not been evaluated yet. This study aimed to assess the effect of obesity on allergic inflammation and T helper-2 profile in an EoE experimental model. Methods:Obesity was induced by high-fat feeding. After 7 weeks of diet, male BALB/c mice were subcutaneously sensitized and orally challenged with OVA.Results: Obesity itself induced a significant mast cell and eosinophil accumulation in the oesophagus, trachea, gut and lung. After allergy induction, this number was higher, when compared to lean-allergic mice. Moreover, obese-allergic mice showed higher remodelling area, in the oesophagus, associated with higher IL-5 and TSLP mRNA expression.In contrast, FoxP3 and IL-10 were less expressed in comparison with lean-allergic mice.In addition, the amount of CD11c + MHCII + PDL1 + dendritic cells was reduced, while the number of CD11c + MHCII + CD80 + DCs and CD3 + CD4 + GATA 3 + IL-4 + cells was increased in obese-allergic mice in the spleen and lymph nodes when compared to lean-allergic mice. Conclusion:Obesity aggravated the immune histopathological characteristics in the EoE experimental model, which was associated with the reduction in the regulatory profile, and the increased inflammatory cells influx, related to the T H 2 profile.Altogether, the data provide new knowledge about obesity as a risk factor, worsening EoE symptoms, and contribute for future treatment strategies for this specific profile. K E Y W O R D Sallergy, eosinophilic oesophagitis, immune response, inflammation, obesity | 245 SILVA et AL.

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Section: Discussionmentioning

confidence: 99%

High‐fat diet‐induced obesity worsens TH2 immune response and immunopathologic characteristics in murine model of eosinophilic oesophagitis

Silva

Oliveira

Ambrósio

et al. 2019

Clin Experimental Allergy

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“…Dellon et al [75] and others have found a dissociation between eosinophils and masts cells [76]. When eosinophil and mast cell counts are combined, there appears to be improved differentiation from gastroesophageal reflux disease (GERD) [75,77,78]. Other reports have noted increases in mast cells compared with GERD as well; however, no differences exist between PPIresponsive and nonresponsive EoE [77,78].…”

Section: Mast Cell Hyperplasia As a Biomarker In Eosinophilic Esophag...mentioning

confidence: 98%

“…When eosinophil and mast cell counts are combined, there appears to be improved differentiation from gastroesophageal reflux disease (GERD) [75,77,78]. Other reports have noted increases in mast cells compared with GERD as well; however, no differences exist between PPI-responsive and nonresponsive EoE [77,78]. When compared with eosinophils, the response of mast cell density to swallowed steroids and diet elimination has been shown to be variable [17 ▪▪ ,26,27 ▪▪ ,28,30,32].…”

Section: Mast Cell Hyperplasia As a Biomarker In Eosinophilic Esophag...mentioning

confidence: 99%

Role of mast cells in eosinophilic esophagitis

Janarthanam

Bolton

Wechsler

2022

Current Opinion in Gastroenterology

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Purpose of reviewThis review will present what is known from recent research on the involvement of mast cells in eosinophilic esophagitis and identify questions requiring further investigation.Recent findingsIn the adults and children with eosinophilic esophagitis, there is increasing evidence that mastocytosis can persist, despite resolution of eosinophilia and is associated with persistent mucosal abnormalities and symptoms. Despite, treatment mast cells have an activated transcriptome. Mast cells likely contribute to epithelial barrier dysfunction, smooth muscle hypertrophy and contraction, and subepithelial fibrosis. It remains unclear whether targeting MCs alone has therapeutic efficacy to improve tissue damage.SummaryMast cells appear to play a key role in eosinophilic esophagitis and serve as a biomarker of mucosal healing in conjunction with eosinophils. Excessive mast cell activation likely contributes to tissue damage in eosinophilic esophagitis and need to be considered as a target of therapy along with eosinophils.

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“…99 Another study reported that tryptase staining of mast cells has been found to differentiate EoE from reflux esophagitis. 100 In addition, the clinical symptom of dysphagia correlates best with esophageal gene transcripts predominantly involving mast cell-specific genes. 101 For other eosinophilic GI disorders, such as eosinophilic gastritis, eosinophilic enteritis, and eosinophilic colitis, the diagnostic criteria are less well defined.…”

Section: Gi Tractmentioning

confidence: 99%

Developing a standardized approach for assessing mast cells and eosinophils on tissue biopsies: A Work Group Report of the AAAAI Allergic Skin Diseases Committee

Zimmermann

Abonia

Dreskin³

et al. 2021

Journal of Allergy and Clinical Immunology

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AAAAI Position Statements,Work Group Reports, and Systematic Reviews are not to be considered to reflect current AAAAI standards or policy after five years from the date of publication. The statement below is not to be construed as dictating an exclusive course of action nor is it intended to replace the medical judgment of healthcare professionals. The unique circumstances of individual patients and environments are to be taken into account in any diagnosis and treatment plan. The statement reflects clinical and scientific advances as of the date of publication and is subject to change.

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Mast Cells Distinguish Eosinophilic Esophagitis in Pediatric Patients

Cited by 7 publications

References 28 publications

High‐fat diet‐induced obesity worsens TH2 immune response and immunopathologic characteristics in murine model of eosinophilic oesophagitis

High‐fat diet‐induced obesity worsens TH2 immune response and immunopathologic characteristics in murine model of eosinophilic oesophagitis

Role of mast cells in eosinophilic esophagitis

Developing a standardized approach for assessing mast cells and eosinophils on tissue biopsies: A Work Group Report of the AAAAI Allergic Skin Diseases Committee

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