Reproducibility of peripapillary retinal nerve fiber layer thickness measurements using Spectral Domain OCT in Brazilian patients

Abstract

Purpose: To evaluate the reproducibility of peripapillary retinal nerve fiber layer (RNFL) thickness measurements in normal eyes and eyes with glaucoma using spectral domain optical coherence tomography (SDOCT). Methods: One eye of 79 normal and 72 glaucoma patients was analyzed. All patients underwent a complete ophthalmological examination, including visual acuity testing; intraocular pressure, slit-lamp examination, indirect ophthalmoscopy; and the glaucoma group underwent achromatic perimetry with the 24-2 SITA Fast Humphrey Field Analyzer. All patients' eyes were scanned using the spectral domain optical coherence tomography -Spectralis ® and one of them was cho sen randomly. Three con secutive circular B-scan centered at the optic disc were performed in one visit. Results: The intraclass correlation coefficient (ICC), coefficient of variation and test-retest variability for the mean retinal nerve fiber layer thickness were respectively: 0.94, 2.56% and 4.85 µm for the normal group and 0.93, 4.65% and 6.61 µm for the glaucomatous group. The intraclass correlation coefficient for retinal nerve fiber layer thickness in all quadrants were all excellent in both groups, with the superior quadrant having the highest ICCs (0.964) in glaucomatous eyes and nasal quadrant measurements having the lowest (0.800), but still excellent in eyes without glaucoma. The coefficient of variation was between 2.56% -8.74% and between 4.65% -11.44% in normal and glaucomatous group respectively. The test-retest variability was between 4.85 µm and 11.51 µm in the normal group and between 6.61 µm and 14.24 µm in the glaucomatous group. The measurements in glaucomatous eyes were more variable than normal eyes. Conclusions: Spectral domain optical coherence tomography showed excellent reproducibility with regard to retinal nerve fiber layer thickness measurements in normal and glaucomatous eyes.Keywords: Diagnostic techniques, ophthalmological; Glaucoma/diagnosis; Tomography, optical coherence/methods; Retinal ganglion cells; Optic nerve/pathology; Nerve fibers; Reproducibility of results RESUMO Objetivo: Avaliar a reprodutibilidade da medida da espessura da camada de fibras nervosas da retina (CFNR) em olhos sem e com glaucoma utilizando-se tomografia de coerência óptica de domínio espectral (spectral domain OCT -SDOCT

Retinal nerve fibre layer thickness values and their associations with ocular and systemic parameters in Black South Africans

“…The iVue-100 SD-OCT (Optovue Inc. Fremont, CA, USA) device was used to measure CCT and RNFL thickness. The iVue-100 SD-OCT scans faster (26,000 A-scans per second) and provides higher resolution cross-sectional images (5 µm) than the TD-OCT. 5,6,7,8 RNFL measurements were performed using the optic nerve head (ONH) iVue-100 (Optovue, Inc.) protocol, which consists of 12 radial scans 3.4 mm in length and 13 concentric ring scans ranging from 1.3 to 4.9 mm. RNFL thickness was measured along a circle of 3.45 mm diameter centred on the optic disc, with the outputs from the measurements being temporal, superior, nasal, and inferior average.…”

“…5 The recently developed spectral domain (SD-OCT) technology 5 has a five times higher image resolution and 60 times faster imaging speed compared with the conventional time domain (TD-OCT) devices, resulting in improved diagnostic accuracy in detecting early glaucoma. 6,7,8 Normative databases of RNFL thickness values from age-matched normals are used to interpret thickness measurements generated by SD-OCT machines. These values vary and cannot be used interchangeably between different SD-OCT machines.…”

Applicability of ISNT and IST rules to the retinal nerve fibre layer using spectral domain optical coherence tomography in early glaucoma

“…The average measurement values for the inferior, superior, nasal and temporal quadrants were noted. The test-retest variability of the RNFL measurement on the Spectralis OCT has been described 19 20. ISNT and IST rules were considered violated only when the difference between the two quadrants was greater than 10 μ. VF and RNFL printouts for normal controls and patients with early glaucoma are as shown (figures 1 and 2).…”

Reproducibility of Disc and Macula Optical Coherence Tomography using the Canon OCT-HS100 as Compared with the Zeiss Cirrus HD-OCT

“…Several studies have been published providing data on interoperator and intraoperator reproducibility of the most common SD-OCTs on the market. For optic disc and macula parameters, intersession reproducibility has been found to vary from about 1.5% to 10% (6, 8-19). In a recent publication on reproducibility (18) evaluating 7 different SD-OCTs, the highest reproducibility was obtained with the Heidelberg Spectralis HRA-OCT and the Zeiss Cirrus HD-OCT.…”