Avaliação da cinética do epitélio corneano com marcadores de proliferação celular

Cresta, Fernando Betty; Alves, Mílton Ruiz

doi:10.1590/s0004-27492007000600013

Cited by 7 publications

(11 citation statements)

References 32 publications

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“…The half-life of Ki67 is short (less than one hour after mitosis). Thus, the use of Ki67 antibody guarantees that only cells that have completed the cell cycle are stained (CRESTA & ALVES, 2007;PAULY et al, 2009), a fact observed in the present study.…”

Section: Discussionsupporting

confidence: 63%

“…Different methods are available for the evaluation of cell proliferation during inflammation (WILSON et al, 2001;BALDWIN & MARSHALL, 2002;PAULY et al, 2009). Ki67 has been shown to be a useful marker for the investigation of cell proliferation in corneal epithelium and stroma of rabbits (CRESTA & ALVES, 2007;PAULY et al, 2009). During corneal wound healing, the epithelium migrates, proliferates and reestablishes its adhesions.…”

Section: Discussionmentioning

confidence: 99%

“…However, it is known that no recent studies have been carried out regarding the impact of such material in corneal cell proliferation by immunohistochemistry. In this context, the cell marker Ki67 has been widely used in the field of the investigative ophthalmology (WILSON et al, 2001;BALDWIN & MARSHALL, 2002;CRESTA & ALVES, 2007;PAULY et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Experimental lamellar keratoplasty in rabbits using microfibrilar cellulose membrane: clinical, morphological and immunohistochemical findings

Macedo¹,

Ribeiro

Conceição

et al. 2010

Cienc. Rural

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The clinical, histopathological and immunohistochemical features of the cornea were investigated in adult male New Zealand rabbits submitted to lamellar keratoplasty with microfibrillar cellulose membrane. Thirty animals were divided into five groups (n=6) and evaluated up to 60 days after surgery. Clinical examination revealed moderate manifestations of edema, blepharospasm and photophobia on the second day, which became mild or disappeared after the seventh day. This period was characterized clinically by repair of the corneal defect. Histopathological analysis showed the presence of a thin layer of squamous cells covering the damaged area as early as 7th day, accompanied by a mild infiltrate of polymorphonuclear cells. Blood vessels were observed in the epithelium after the 15th day, which had regressed by day 48. Ki67 antibody labeling showed an increase of proliferating cells in the epithelium by the 15th day and in the stroma by day 30. Remodeling and epithelial adhesion were observed during this period. Microfibrillar cellulose membrane (Bionext®) used for lamellar keratoplasty was found to yield good results considering the good integration of the implant.

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Section: Discussionsupporting

confidence: 63%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Experimental lamellar keratoplasty in rabbits using microfibrilar cellulose membrane: clinical, morphological and immunohistochemical findings

Macedo¹,

Ribeiro

Conceição

et al. 2010

Cienc. Rural

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“…We chose to study the corneas of rabbits due to their similarity to the human cornea. They have [21][22][23] . MMC, a drug used as adjuvant therapy in many ophthalmic procedures, acts as an inhibitor of cell proliferation and has been linked to postoperative complications such as corneal erosion, conjunctival hyperaemia, blepharospasm, corneal and scleral thinning, and perforations 8,10 .…”

Section: Discussionmentioning

confidence: 99%

Influence of intraoperative episcleral application of topic mitomycin C on proliferation and differentiation of rabbit corneal and conjunctival epithelial cells

Potério

José

Amstalden

et al. 2014

Revista Brasileira De Oftalmologia

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Influência da aplicação intraoperatória de mitomicina C tópica episcleral na proliferação e diferenciação de células epiteliais córneo-conjuntivais de coelhos RESUMOObjetivo: Avaliar a influência da mitomicina C a 0,02% (MMC), aplicada em dose única por 3 minutos, na proliferação e diferenciação das células epiteliais da córnea de coelhos. Métodos: A MMC tópica foi aplicada na episclera da área límbica temporal, mediante tecido epitelial corneano intacto (um olho) e após desepitelização epitelial parcial da córnea (outro olho). Durante o procedimento cirúrgico, MMC ou solução fisiológica 0,9% (SF) foi aplicada e a solução escolhida para cada animal foi determinada por sorteio. Os animais foram divididos em grupo A (20 olhos), grupo B (16 olhos) e grupo C (14 olhos). Foram sacrificados respectivamente em 4º, 15º e 45º dia de pós-operatório. Para estimular a proliferação celular, os animais do grupo C foram submetidos à desepitelização central da córnea 3 dias antes do dia do sacrifício. Marcadores de diferenciação celular (AE1, AE3 e AE5) e de proliferação (5-Bromo-2-Deoxiuridina, BrdU) foram utilizados. Nas lâminas coradas com BrdU, as áreas nasal, temporal e central foram delimitadas. O número de células coradas pela BrdU foram contadas nos 3 diferentes campos e a média aritmética de cada área foi analisada estatisticamente. Resultados: Houve diferença estatística entre MMC e SF nas áreas central e temporal da córnea previamente desepitelizada em todos os grupos. Não houve diferença estatística durante a análise de diferenciação celular. Conclusão: A MMC na dose de 0,02%, aplicada por 3 minutos sobre a episclera, interfere na proliferação celular da área exposta à droga e previamente desepitelizada. Não interfere na diferenciação celular e sua ação possui efeito prolongado.

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“…Sections (4-µm) were stained using hematoxylineosin (HE) or subjected to immunohistochemistry to detect cell proliferation (Ki-67 [clone MIB-1]; DAKO, CA, USA) (CRESTA & ALVES, 2007). Optimum dilution of the antibody was 1:50.…”

Section: Histopathology and Immunohistochemistrymentioning

confidence: 99%

Lamellar keratoplasty in rabbits using an allogeneic free omental graft and omentum-derived mesenchymal cells associated with the canine amniotic membrane

et al. 2016

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The objective of this research was to evaluate the clinical and microscopic effects in rabbits of lamellar keratoplasty using allogeneic omentum associated with canine amniotic membrane (AM). Rabbits were divided into two groups: one received the allogeneic free omental graft covered with the AM (OM-graft group), while the other received the AM graft containing omental mesenchymal cells (OM-cell group). Clinical signs were evaluated on different postoperative days. After the clinical assessments, the rabbits were euthanized and their corneas were obtained for histopathology and immunohistochemistry (Ki-67, marker for proliferation). Both groups showed chemosis, blepharospasm, eye discharge, hyperemia, and corneal opacity/edema. Neovascularization was observed in the OM-cell group. Histopathological evaluation revealed epithelial islands within the stroma of OM-cell samples. Thirty days after surgery, complete corneal re-epithelialization had occurred in both groups. The OM-cell group showed more Ki-67 positive cells. The free omentum and its cells, combined with the AM, contributed to corneal repair, a process that was completed 30 days after lamellar keratoplasty.

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Avaliação da cinética do epitélio corneano com marcadores de proliferação celular

Cited by 7 publications

References 32 publications

Experimental lamellar keratoplasty in rabbits using microfibrilar cellulose membrane: clinical, morphological and immunohistochemical findings

Experimental lamellar keratoplasty in rabbits using microfibrilar cellulose membrane: clinical, morphological and immunohistochemical findings

Influence of intraoperative episcleral application of topic mitomycin C on proliferation and differentiation of rabbit corneal and conjunctival epithelial cells

Lamellar keratoplasty in rabbits using an allogeneic free omental graft and omentum-derived mesenchymal cells associated with the canine amniotic membrane

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