A fibrin-related line of research and theoretical possibilities for the use of fibrin glue as a temporary basal membrane in non-perforated corneal ulcers and in photorefractive keratectomy (PRK)-operated corneas

Bonatti, José Américo; Bechara, Samir Jacob; Dall'Col, Marcelo; Cresta, Fernando Betty; Carricondo, Pedro Carlos; Kara‐José, Newton

doi:10.1590/s0004-27492007000500029

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Temporary Basement Membrane1

Introduction1

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2019

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Cited by 7 publications

(2 citation statements)

References 22 publications

(32 reference statements)

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“…Bonatti proposed the use of fibrin as a temporary basal membrane on nonperforated corneas for photorefractive keratectomy and non-infected autoimmune corneal ulcers [112]. The advantage of the fibrin temporary basal membrane is in its ability to facilitate epithelial migration and inhibition of fibrinolysis by the plasmin inhibitor tranexamic acid, which prevents recurrence of the ulcers.…”

Section: Temporary Basement Membranementioning

confidence: 99%

Tissue adhesives in ocular surgery

Park

Champakalakshmi

Panengad

et al. 2011

Expert Review of Ophthalmology

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Section: Temporary Basement Membranementioning

confidence: 99%

Tissue adhesives in ocular surgery

Park

Champakalakshmi

Panengad

et al. 2011

Expert Review of Ophthalmology

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“…When used in wounds, these exogenous components can help endogenous proteins to reach hemostasis (Laurens, 2006). The principle is that fibrinogen is converted into fibrin monomers by thrombin, then fibrin monomers are modified by factor XIII and calcium chloride becomes a fibrin polymer as the final form of fibrin clots through cross-linking (Bonatti et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Temperature effect on expression of recombinant human prethrombin-2 in Escherichia coli BL21(DE3) ArcticExpress

Silaban

Simorangkir

Gaffar

et al. 2019

JPKim

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Many proteins produced in E. coli accumulate in inclusion bodies. This study aims to detect the role of temperature in reducing the formation of inclusion bodies during recombinant human prethrombin-2expressed in E. coli BL21 (DE3) Arctic Express host. In this study, we created a host growth curve to find out the right time to add an inducer. The inducer used in our experiment was IPTG 0.1 mM. The fermentation process use a temperature of 12°C and 22°C. The results showed that recombinant human prethrombin-2 was successfully expressed as protein soluble using an optimum temperature of 12°C in E. coli BL21 (DE3) Arctic Express. It was indicated from the 63kDa protein band obtained from the soluble fraction on SDS-PAGE. The higher temperature of fermentation increased the amount of protein in the insoluble fraction due. It concluded that the fermentation temperature affect the rate of prethrombin-2 expression.

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