Citologia de impressão no diagnóstico de infecção corneana por Acanthamoeba: relato de caso

Barros, João; Mascaro, Vera Lúcia Degaspare Monte; Lowen, Márcia Serva; Martins, Maria Cristina; Foronda, Annette Silva

doi:10.1590/s0004-27492007000200028

Cited by 8 publications

(3 citation statements)

References 15 publications

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“…Light microscopy is an efficient means to detect Acanthamoeba in corneal scrapings, in biopsy samples, and in keratoplasty specimens. Acanthamoeba can be detected in wet-mount preparations of corneal scraping, using 10% KOH [126–132]. Moreover, impression cytology was able to remove amoebic specimens of an AK patient [132].…”

Section: Acanthamoeba Spp and Amoebic Keratitis (Ak)mentioning

confidence: 99%

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Diagnosis of Infections Caused by Pathogenic Free-Living Amoebae

Rocha-Azevedo

Tanowitz

Marciano‐Cabral

2009

Interdisciplinary Perspectives on Infectious Diseases

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Naegleria fowleri, Acanthamoeba spp., Balamuthia mandrillaris, and Sappinia sp. are pathogenic free-living amoebae. N. fowleri causes Primary Amoebic Meningoencephalitis, a rapidly fatal disease of the central nervous system, while Acanthamoeba spp. and B. mandrillaris cause chronic granulomatous encephalitis. Acanthamoeba spp. also can cause cutaneous lesions and Amoebic Keratitis, a sight-threatening infection of the cornea that is associated with contact lens use or corneal trauma. Sappinia pedata has been identified as the cause of a nonlethal case of amoebic encephalitis. In view of the potential health consequences due to infection with these amoebae, rapid diagnosis is critical for early treatment. Microscopic examination and culture of biopsy specimens, cerebral spinal fluid (CSF), and corneal scrapings have been used in the clinical laboratory. For amoebic keratitis, confocal microscopy has been used to successfully identify amoebae in corneal tissue. More recently, conventional and real-time PCR assays have been developed that are sensitive and specific for the amoebae. In addition, multiplex PCR assays are available for the rapid identification of these pathogens in biopsy tissue, CSF, and corneal specimens.

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Section: Acanthamoeba Spp and Amoebic Keratitis (Ak)mentioning

confidence: 99%

“…Acanthamoeba can be detected in wet-mount preparations of corneal scraping, using 10% KOH [126–132]. Moreover, impression cytology was able to remove amoebic specimens of an AK patient [132]. H & E and Giemsa stains have been used successfully to detect both trophozoites and cysts of Acanthamoeba .…”

Section: Acanthamoeba Spp and Amoebic Keratitis (Ak)mentioning

confidence: 99%

Diagnosis of Infections Caused by Pathogenic Free-Living Amoebae

Rocha-Azevedo

Tanowitz

Marciano‐Cabral

2009

Interdisciplinary Perspectives on Infectious Diseases

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“…Thus, as conjunctiva is an easily accessible tissue source for cytological examination, the analysis of the features from melanocytic cells by the noninvasive and affordable impression cytology (IC) can help in the differentiation of benign from malignant pigmented tumors of the bulbar conjunctiva (7)(8) . IC, first described in 1977 for the collection of mucin and cells from the conjunctiva (9) , had other diagnostic possibilities, such as: ocular surface squamous neoplasia (10)(11)(12)(13) , dry eyes (14) , vitamin A deficiency (15) , allergic conjunctivitis (16) , limbal stem cell failure (17) and microbiological infections (18) . The aim of this study was to report the IC features of conjunctival nevi.…”

Section: Introductionmentioning

confidence: 99%

Impression cytology features of conjunctival nevi reported as more noticeable

Barros¹,

Lowen²,

Mascaro³

et al. 2009

Arq. Bras. Oftalmol.

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Optical microscopy analysis of the impression cytology specimens confirmed the clinical diagnosis by demonstrating typical histopathological features of the superficial layers from conjunctival nevi in 91.4% of the cases. For amelanotic nevi IC can also allow a differential diagnosis from other nonpigmented lesions. The technique does not replace histopathological examination, but additionally, may assist in evaluating nevus cells in children and adults.

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