Correlation between clinical diagnosis and PCR analysis of serum, aqueous, and vitreous samples in patients with inflammatory eye disease

Matos, Kimble; Muccioli, Cristina; Belfort, Rubens; Rizzo, Luiz Vicente

doi:10.1590/s0004-27492007000100020

Cited by 43 publications

(40 citation statements)

References 29 publications

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“…Our patients with presumed ocular tuberculosis presented negative PCR results suggesting that these patients developed uveitis for other reasons or that they had very low number of organisms in ocular fluids as well in plasma and total blood. In 2007, a study in our Institution presented one patient with presumed ocular tuberculosis that had a positive PCR reaction both in the serum and in the vitreous samples (22) . In addition, one patient, who is also HIV + , has tested positive for HSV1 infection and also by T. gondii and could have had a diagnosis of CMV due to immunocompromise because though the features of the lesion caused by the tendency for severe uveitis clinical hypothesis that toxoplasmosis is also a feature of infection in immunocompromised patients (23) .…”

Section: Discussionmentioning

confidence: 99%

Real-time PCR in infectious uveitis as an alternative diagnosis

Santos¹,

Commodaro²,

Souza³

et al. 2011

Arq. Bras. Oftalmol.

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Purpose: Uveitis is a major visual impairment disease affecting parts or the entire uveal tract and occasionally the sclera, the cornea or the optic nerve. The disease is a major cause of ocular morbidity and blindness in immunocompetent and immunocompromised patients. In this work we analyzed the sensitivity and specificity of realtime PCR to detect the etiological agent from blood, plasma, vitreous and aqueous humor and compared with the diagnostic hypothesis. Methods: Twenty-seven patients (13 male) were studied and Real-time PCR method was used for the detection of herpes simplex virus 1 (HSV-1), herpes simplex virus 2 (HSV-2), varicella zoster virus (VZV), cytomegalovirus (CMV), Mycobacterium tuberculosis (TB) and Toxoplasma gondii (Toxo) in the aqueous humor as well as in the vitreous, blood and plasma. Results: Our results showed the presence of Toxo, CMV, VZV or HSV-2 in 19.2% of aqueous humor samples, and in 30% of vitreous humor samples. In plasma and blood samples, only CMV was detected (11.1% and 3.7%, respectively). Conclusion: Real-time PCR was able to detect and to confirm diagnostic hypothesis in uveitis. Our data also confirms that vitreous humor is the best source for molecular diagnosis of infectious uveitis but indicates aqueous humor samples that are easier to obtain may also be appropriate to be tested by Real-time PCR. Keywords

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Section: Discussionmentioning

confidence: 99%

Real-time PCR in infectious uveitis as an alternative diagnosis

Santos¹,

Commodaro²,

Souza³

et al. 2011

Arq. Bras. Oftalmol.

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“…Infection by T. gondii can be diagnosed indirectly with serological methods or directly by histology, isolation of the parasite or its material (polymerase chain reaction (PCR), hybridization) (2,6,21) . The diagnosis of ocular toxoplasmosis is mainly clinical (3,22) .…”

Section: Discussionmentioning

confidence: 99%

“…Histologically, ocular toxoplasmosis often presents extensive granulomatous inflammatory infiltration of the choroid and areas of necrosis under the retinal pigment epithelium (3,26) . Furthermore, the parasite's DNA can be identified in vitreous and humour samples using PCR (21,28) . Serum levels of chemokines (CXCL8) can also be used, mainly during follow-up (29) .…”

Section: Discussionmentioning

confidence: 99%

Use of CD25 as an immunohistochemical marker for acquired ocular toxoplasmosis

Miyamoto¹,

Neto²,

Cesare³

et al. 2010

Arq. Bras. Oftalmol.

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“…The use of real-time PCR as a diagnostic tool for infectious uveitis has been demonstrated by many groups (11,(19)(20)(21) . In this study, qPCR identified T. gondii infection in 37.21% of the aqueous humor samples, confirming the clinical hypothesis of toxoplasmic active focal necrotizing retinochoroiditis.…”

Section: Discussionmentioning

confidence: 99%

“…In this study, qPCR identified T. gondii infection in 37.21% of the aqueous humor samples, confirming the clinical hypothesis of toxoplasmic active focal necrotizing retinochoroiditis. A previous study reported that aqueous PCR analyses are useful in AIDS patients with ocular toxoplasmosis (sensitivity of 75%) (19) and demonstrated that the aqueous humor is the best source for identifying T. gondii infection. Another study also detected T. gondii DNA (38%) by PCR in aqueous humor samples (16) .…”

Section: Discussionmentioning

confidence: 99%

Detection ofToxoplasma gondiiDNA in peripheral blood and aqueous humor of patients with Toxoplasmic active focal necrotizing retinochoroiditis using real-time PCR

Santos¹,

Nascimento²,

Muccioli³

et al. 2015

Arquivos Brasileiros De Oftalmologia

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Purpose: To evaluate the ability of real-time quantitative PCR (qPCR) for detecting Toxoplasma gondii DNA in the peripheral blood and aqueous humor of patients with toxoplasmic active focal necrotizing retinochoroiditis. Methods: Fifty-five patients with infectious uveitis seen from 2009 to 2013 at the Department of Ophthalmology and Visual Sciences of the Federal University of São Paulo were enrolled in this study. Forty-three patients had toxoplasmic active focal necrotizing retinochoroiditis, and the remaining 12 had non-toxoplasmic infectious uveitis and served as controls. qPCR analysis for T. gondii DNA was performed on the patients' peripheral blood and aqueous humor samples. Results:The qPCR was positive for T. gondii DNA in 37.21% (16/43) of the aqueous humor samples and 2.33% (1/43) of the peripheral blood samples; further, 16.27% (7/43) of the patients had positive results in both their blood and aqueous humor samples. Conclusion: qPCR was able to detect T. gondii DNA in patients with toxoplasmic active focal necrotizing retinochoroiditis in the blood as well as the aqueous humor and can help with the diagnosis of the disease.

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Correlation between clinical diagnosis and PCR analysis of serum, aqueous, and vitreous samples in patients with inflammatory eye disease

Cited by 43 publications

References 29 publications

Real-time PCR in infectious uveitis as an alternative diagnosis

Real-time PCR in infectious uveitis as an alternative diagnosis

Use of CD25 as an immunohistochemical marker for acquired ocular toxoplasmosis

Detection ofToxoplasma gondiiDNA in peripheral blood and aqueous humor of patients with Toxoplasmic active focal necrotizing retinochoroiditis using real-time PCR

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