Use of CD25 as an immunohistochemical marker for acquired ocular toxoplasmosis

Miyamoto, Cristina; Neto, Rubens Belfort; Cesare, Sebastian Di; Belfort, Rubens; Burnier, Miguel N.

doi:10.1590/s0004-27492010000500011

Cited by 6 publications

(6 citation statements)

References 24 publications

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“…This finding indicated that IFN‐ γ and IL‐4 co‐expressing CD4 T cells were not naive T cells. Moreover, the CD25 and CD69 expression levels on the IFN‐ γ and IL‐4 cells reflected that this cell population was in an activated state, as reported by Miyamoto et al . More interesting is that the IFN‐ γ ‐ and IL‐4‐expressing cells expressed similar levels of these cell surface markers as Th1 and Th2 cells, though some differences existed between these cell populations as shown in Fig.…”

Section: Discussionsupporting

confidence: 74%

Characteristics of Schistosoma japonicum infection induced IFN‐γ and IL‐4 co‐expressing plasticity Th cells

et al. 2016

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Section: Discussionsupporting

confidence: 74%

Characteristics of Schistosoma japonicum infection induced IFN‐γ and IL‐4 co‐expressing plasticity Th cells

et al. 2016

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“…The regulatory activity on Th1, Th2, and CD8 + cells is mediated through the local secretion of TGF-ß ad IL-10 [ 44 ]. In the eye, infiltrates of CD4 + CD25 + T cells are specifically found in the retina of patients with recently acquired toxoplasmosis indicating that recently orally ingested parasite leads to the presence of these immune-regulatory cells in their infected retinas [ 26 ]. Their presence was explained as a mechanism to modulate the retinal damage, through a regulatory effect on the Th-1 response during the retinochoroiditis [ 14 ].…”

Section: Discussionmentioning

confidence: 99%

Is reactivation of toxoplasmic retinochoroiditis associated to increased annual rainfall?

et al. 2013

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Background: Reactivation of toxoplasmic retinochoroiditis is the most frequent form of uveitis in Misiones, Argentina. Fluctuations in the number of patients consulting with this type of uveitis were detected during the last decade. Since the province was consecutively exposed to rainy and dry periods over the last years, we decided to explore whether a relationship between reactivation of toxoplasmic retinochoroiditis and rain might be established according to the data registered during the 2004–2010 period. Results: The frequency of toxoplasmic reactivation episodes increases when precipitation increases (mostly in second and fourth trimesters of each year). Analysis of the independent variables demonstrates that precipitation is a significant predictor of the frequency of reactivation episodes. Although registered toxoplasmic reactivations were more frequent during the third trimester of the year, the association between the third trimester and the reactivation episodes did not reach statistical significance. Conclusion: Prolonged and intense rainfall periods were significantly associated with the reactivation of toxoplasmic retinochoroiditis. Changes promoted by this climatic condition on both the parasite survival in the soil as well as a putative effect on the host immune response due to other comorbidities are discussed.

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“…Data is qualitative [14] Measure biological function Assessment of signal transduction (e.g., arrays, such as ZeptoMARK), cell proliferation or differentiation induced as a result of ligand-receptor binding Provides indirect analysis of ligand-receptor interaction [2,15,16] Most such assays quantify complexes indirectly by measuring the levels of each molecular component independently or measuring the biological outcome of the complexes. Assays geared towards quantifying ligand-receptor complexes, such as FRET, are generally difficult to apply in a high-throughput platform like that required for analysis of a large numbers of samples or clinical diagnosis [1,[17][18][19][20][21][22][23]. The result is that the biologically and clinically significant parameters associated with ligand-receptor complexes, such as the fractional occupancy of a receptor by its ligand, remain understudied and underappreciated.…”

Section: Confocal Microscopymentioning

confidence: 99%

“…Most such assays quantify complexes indirectly by measuring the levels of each molecular component independently or measuring the biological outcome of the complexes. Assays geared towards quantifying ligand–receptor complexes, such as FRET, are generally difficult to apply in a high-throughput platform like that required for analysis of a large numbers of samples or clinical diagnosis [ 1 , 17 , 18 , 19 , 20 , 21 , 22 , 23 ]. The result is that the biologically and clinically significant parameters associated with ligand–receptor complexes, such as the fractional occupancy of a receptor by its ligand, remain understudied and underappreciated.…”

Section: Introductionmentioning

confidence: 99%

Quantification of Receptor Occupancy by Ligand—An Understudied Class of Potential Biomarkers

Veeramani

Weiner

2020

Cancers

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Molecular complexes, such as ligand–receptor complexes, are vital for both health and disease and can be shed into the circulation in soluble form. Relatively little is known about the biology of soluble ligand–receptor complexes. The functional importance of such complexes and their potential use as clinical biomarkers in diagnosis and therapy remains underappreciated. Most traditional technologies used to study ligand–receptor complexes measure the individual levels of soluble ligands or receptors rather than the complexes themselves. The fraction of receptors occupied by ligand, and the potential clinical relevance of such information, has been largely overlooked. Here, we review the biological significance of soluble ligand–receptor complexes with a specific focus on their potential as biomarkers of cancer and other inflammatory diseases. In addition, we discuss a novel RNA aptamer-based technology, designated ligand–receptor complex-binding aptamers (LIRECAP), that can provide precise measurement of the fraction of a soluble receptor occupied by its ligand. The potential applicability of the LIRECAP technology as a biomarker discovery platform is also described.

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Use of CD25 as an immunohistochemical marker for acquired ocular toxoplasmosis

Cited by 6 publications

References 24 publications

Characteristics of Schistosoma japonicum infection induced IFN‐γ and IL‐4 co‐expressing plasticity Th cells

Characteristics of Schistosoma japonicum infection induced IFN‐γ and IL‐4 co‐expressing plasticity Th cells

Is reactivation of toxoplasmic retinochoroiditis associated to increased annual rainfall?

Quantification of Receptor Occupancy by Ligand—An Understudied Class of Potential Biomarkers

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