“…However, in a later study by Hamano et al (1990) and in a multicentre study reported by Sakamoto et al (1993), it is stated that the subjects were asked to keep their eyes open for the 15 s whilst the cotton thread was in contact with the eyelid margin, and even to blink naturally. Similar protocol descriptions can be found in numerous further studies on the PRT (Little and Bruce, 1994;Kwong and Cho, 1998;Albietz et al, 2002;Labetoulle et al, 2002;Cho and Chan, 2003;Glasson et al, 2003Glasson et al, , 2006Yeo et al, 2003;Miller et al, 2004;Hida et al, 2005). However, in some studies, it is unclear which of the two approaches were used partly because it is not clearly stated but either the early Hamano et al (1983) study or that of Cho (1993) is directly or indirectly cited as the method (Mainstone et al, 1996;Golding et al, 1997;Quevedo et al, 2000;Sorbara et al, 2004;Tamer et al, 2005); both these method papers report that the test was conducted with a closed eye.…”