Bacteriocin-like activity of oral Fusobacterium nucleatum isolated from human and non-human primates

Júnior, Élerson Gaetti-Jardim; Ávila-Campos, Mário Júlio

doi:10.1590/s0001-37141999000400009

Cited by 4 publications

(1 citation statement)

References 19 publications

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“…Bacteriocin or bacteriocin-like activities have also been reported and well documented for other oral bacteria, such as P. intermedia (194), Capnocytophaga ochracea (145), A. actinomycetemcomitans (72), Haemophilus influenzae (116), F. nucleatum (56), and E. corrodens (2). However, their biochemical properties and genetic determinants have not yet been well characterized.…”

Section: Bacteriocins and Their Role In Interspecies Interaction Withmentioning

confidence: 99%

Interspecies Interactions within Oral Microbial Communities

Kuramitsu

Lux

et al. 2007

Microbiol Mol Biol Rev

489

395

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SUMMARY While reductionism has greatly advanced microbiology in the past 400 years, assembly of smaller pieces just could not explain the whole! Modern microbiologists are learning “system thinking” and “holism.” Such an approach is changing our understanding of microbial physiology and our ability to diagnose/treat microbial infections. This review uses oral microbial communities as a focal point to describe this new trend. With the common name “dental plaque,” oral microbial communities are some of the most complex microbial floras in the human body, consisting of more than 700 different bacterial species. For a very long time, oral microbiologists endeavored to use reductionism to identify the key genes or key pathogens responsible for oral microbial pathogenesis. The limitations of reductionism forced scientists to begin adopting new strategies using emerging concepts such as interspecies interaction, microbial community, biofilms, polymicrobial disease, etc. These new research directions indicate that the whole is much more than the simple sum of its parts, since the interactions between different parts resulted in many new physiological functions which cannot be observed with individual components. This review describes some of these interesting interspecies-interaction scenarios.

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Section: Bacteriocins and Their Role In Interspecies Interaction Withmentioning

confidence: 99%

Interspecies Interactions within Oral Microbial Communities

Kuramitsu

Lux

et al. 2007

Microbiol Mol Biol Rev

489

395

View full text Add to dashboard Cite

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Design Strategies for Integrated β‐Galactosidase Purification Processes

et al. 2014

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The operational conditions for an aqueous two‐phase system (ATPS) for β‐galactosidase purification were optimized and applied to the design of a purification strategy as an alternative to the primary purification steps. The ATPS proved to be suitable for the recovery and primary enzyme purification. The purification process design developed by ATPS, diafiltration, ion exchange, and diafiltration/ultrafiltration was successful, yielding a more than tenfold purification. The purification strategy design resulted in a powerful integrated purification and recovery process, an evidence of the potential for a scale‐up of the β‐galactosidase purification process.

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PEG–salt aqueous two-phase systems: an attractive and versatile liquid–liquid extraction technology for the downstream processing of proteins and enzymes

Glyk

Scheper

Beutel

2015

Appl Microbiol Biotechnol

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Nowadays, there is an increasing demand to establish new feasible, efficient downstream processing (DSP) techniques in biotechnology and related fields. Although several conventional DSP technologies have been widely employed, they are usually expensive and time-consuming and often provide only low recovery yields. Hence, the DSP is one major bottleneck for the commercialization of biological products. In this context, polyethylene glycol (PEG)-salt aqueous two-phase systems (ATPS) represent a promising, efficient liquid-liquid extraction technology for the DSP of various biomolecules, such as proteins and enzymes. Furthermore, ATPS can overcome the limitations of traditional DSP techniques and have gained importance for applications in several fields of biotechnology due to versatile advantages over conventional DSP methods, such as biocompatibility, technical simplicity, and easy scale-up potential. In the present review, various practical applications of PEG-salt ATPS are presented to highlight their feasibility to operate as an attractive and versatile liquid-liquid extraction technology for the DSP of proteins and enzymes, thus facilitating the approach of new researchers to this technique. Thereby, single- and multi-stage extraction, several process integration methods, as well as large-scale extraction and purification of proteins regarding technical aspects, scale-up, recycling of process chemicals, and economic aspects are discussed.

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Bacteriocin-like activity of oral Fusobacterium nucleatum isolated from human and non-human primates

Cited by 4 publications

References 19 publications

Interspecies Interactions within Oral Microbial Communities

Interspecies Interactions within Oral Microbial Communities

Design Strategies for Integrated β‐Galactosidase Purification Processes

PEG–salt aqueous two-phase systems: an attractive and versatile liquid–liquid extraction technology for the downstream processing of proteins and enzymes

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